Risks of antibiotic residues in milk following intramammary and intramuscular treatments in dairy sheep

Pengov, A.; Kirbiš, Andrej

doi:10.1016/j.aca.2008.09.021

Cited by 23 publications

(14 citation statements)

References 8 publications

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“…Domingues et al (2006) Morais et al (2011) reported that the practice of not fully milking the mothers at the end of lactation is a relevant factor for predisposition to the disease. According to Pengov and Kirbis (2009), administration of antimicrobial agents in the drying phase of the sheep has two objectives: to eliminate existing subclinical intramammary infections, which can cause recrudescence of clinical disease and prevent new infections during the dry period, when sheep are particularly susceptible.…”

Section: Resultsmentioning

confidence: 99%

OCCURRENCE OF ETIOLOGICAL AGENTS CAUSING SUBCLINICAL MASTITIS IN MORADA NOVA AND SANTA INÊS EWES / Ocorrência dos agentes etiológicos causadores de mastite subclínica em ovelhas das raças morada nova e Santa Inês.

Santana

Zafalon

Esteves

et al. 2013

Ars Vet

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SUMMARYThis study aimed to determine the occurrence of the etiologic agents of subclinical mastitis in Santa Inês and Morada Nova ewes and their susceptibility to developing the disease when submitted to the same management conditions. We analyzed 250 mammary glands of 130 Santa Inês ewes and 143 mammary glands of 77 Morada Nova ewes. The California Mastitis Test, somatic cell counts and microbiological analysis were performed at the moment of drying off. The occurrences of subclinical mastitis in different breeds were analyzed using the chi-square test by adjusting the values according to the Yates continuity correction. The infectious subclinical mastitis was present in 33.1% and 35. RESUMOEste trabalho objetivou determinar a ocorrência dos agentes etiológicos da mastite subclínica em ovelhas das raças Santa Inês e Morada Nova e as susceptibilidades das duas raças em desenvolver a doença quando submetidas às mesmas condições de manejo. Analisou-se 250 mamas de 130 ovelhas da raça Santa Inês e 143 mamas de 77 ovelhas da raça Morada Nova. No momento da secagem, realizaram-se o California Mastitis Test, contagem de células somáticas e análises microbiológicas. As ocorrências da mastite subclínica nas diferentes raças foram analisadas utilizando o teste de qui-quadrado ajustando os valores de acordo com a correção de continuidade de Yates. As ocorrências de animais com mastite subclínica infecciosa foram de 33,1% e de 35,1% nas ovelhas Santa Inês e Morada Nova, respectivamente. Dentre as mamas avaliadas das ovelhas Santa Inês, 20,4% apresentaram mastite subclínica com a seguinte etiologia infecciosa: Staphylococcus coagulase-negativos (SCN

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Section: Resultsmentioning

confidence: 99%

OCCURRENCE OF ETIOLOGICAL AGENTS CAUSING SUBCLINICAL MASTITIS IN MORADA NOVA AND SANTA INÊS EWES / Ocorrência dos agentes etiológicos causadores de mastite subclínica em ovelhas das raças morada nova e Santa Inês.

Santana

Zafalon

Esteves

et al. 2013

Ars Vet

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“…This illegal act is becoming more common in China (Cui et al, 2007;Xu et al, 2010). If consumed by humans, β-lactamase can lead to antibiotic resistance and thus high susceptibility to infections (Kantiani et al, 2009;Pengov and Kirbis, 2009). Moreover, addition of β-lactamase enables the presence of antibiotics to be concealed, thereby encouraging the overuse of antibiotics during milk production, storage, and transportation.…”

Section: Introductionmentioning

confidence: 95%

A rapid HPLC method for indirect quantification of β-lactamase activity in milk

Zhou

Wang

Zhao

et al. 2015

Journal of Dairy Science

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To circumvent the strictly regulated limits of antibiotics in milk, illegal addition of β-lactamase to lower the antibiotic levels in milk has been reported recently in China. Herein, we describe a fast, sensitive, and robust HPLC-UV method for the determination of β-lactamase activity in milk, based on an indirect quantification strategy. The test milk sample was mixed with a known amount of penicillin G, a specific substrate of β-lactamase. After incubation, an aliquot of the mixture was injected into the HPLC-UV system to quantify the remaining penicillin G in less than 10 min. Comparative analysis of the amount of penicillin G before and after incubation was used to indirectly deduce the activity of β-lactamase in the test sample. This method was successfully applied to milk products with different fat percentages, resulting in a detection limit of 0.6 U/mL. Good recoveries, ranging from 94 to 105%, were obtained from blank milk samples spiked with β-lactamase at levels of 2 to 50 U/mL, with relative standard deviations <6%. A good correlation was demonstrated between the HPLC method and the conventional culture-based assay. Using this method, the activity changes in β-lactamase during milk pasteurization, sterilization, and storage were investigated. The advantages of low-cost, accurate quantification and easily accessible instrumentation make the proposed method an ideal alternative for high-throughput routine analysis in the dairy industry.

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“…However, extrapolation of data from bovine studies in regard to IMM administration of drugs in small ruminants may not be appropriate owing to interspecies differences and differences in the mastitis status of individual animals. 103,104 Although the gross composition of caprine milk is similar to that of bovine milk, there are some differences that may affect the absorption, distribution, and elimination of drugs following IMM infusion. The composition of the casein and whey protein fractions of caprine milk differs from that of bovine milk, and caprine milk has a higher proportion of free fatty acids and smaller fat globules than bovine milk.…”

Section: Combined Florfenicol-flunixin Meglumine Formulation-mentioning

confidence: 99%