OCCURRENCE OF ETIOLOGICAL AGENTS CAUSING SUBCLINICAL MASTITIS IN MORADA NOVA AND SANTA INÊS EWES / Ocorrência dos agentes etiológicos causadores de mastite subclínica em ovelhas das raças morada nova e Santa Inês.

Abstract

SUMMARYThis study aimed to determine the occurrence of the etiologic agents of subclinical mastitis in Santa Inês and Morada Nova ewes and their susceptibility to developing the disease when submitted to the same management conditions. We analyzed 250 mammary glands of 130 Santa Inês ewes and 143 mammary glands of 77 Morada Nova ewes. The California Mastitis Test, somatic cell counts and microbiological analysis were performed at the moment of drying off. The occurrences of subclinical mastitis in different breeds were analyzed using the chi-square test by adjusting the values according to the Yates continuity correction. The infectious subclinical mastitis was present in 33.1% and 35. RESUMOEste trabalho objetivou determinar a ocorrência dos agentes etiológicos da mastite subclínica em ovelhas das raças Santa Inês e Morada Nova e as susceptibilidades das duas raças em desenvolver a doença quando submetidas às mesmas condições de manejo. Analisou-se 250 mamas de 130 ovelhas da raça Santa Inês e 143 mamas de 77 ovelhas da raça Morada Nova. No momento da secagem, realizaram-se o California Mastitis Test, contagem de células somáticas e análises microbiológicas. As ocorrências da mastite subclínica nas diferentes raças foram analisadas utilizando o teste de qui-quadrado ajustando os valores de acordo com a correção de continuidade de Yates. As ocorrências de animais com mastite subclínica infecciosa foram de 33,1% e de 35,1% nas ovelhas Santa Inês e Morada Nova, respectivamente. Dentre as mamas avaliadas das ovelhas Santa Inês, 20,4% apresentaram mastite subclínica com a seguinte etiologia infecciosa: Staphylococcus coagulase-negativos (SCN

Relationship between virulence factor genes in coagulase-negative Staphylococcus spp. and failure of antimicrobial treatment of subclinical mastitis in sheep

“…reported a CNS occurrence rate in milk samples from Santa Inês sheep of 64.3%. A similar rate was also reported by other authors (Pereira et al 2014, Santana et al 2013. CNS can also be isolated from animals with clinical mastitis (Lucheis et al 2010).…”

Genotypic identification and antimicrobial susceptibility profiles of coagulase-negative staphylococci isolated from sheep’s milk

“…Several studies suggest that CoNS are the most common cause of sheep mastitis (BARILLET et al, 2001;SANTANA et al, 2013), with a prevalence ranging from 25 to 93% in ewes with intramammary infection (BERGONIER & BERTHELOT, 2003).…”

“…Mastitis caused by coagulase-negative staphylococci (CoNS) has been reported as one of the leading factors affecting milk and dairy production in sheep (BARILLET et al, 2001;SANTANA et al, 2013). In its subclinical form, sheep mastitis is undetectable except for a persistent reduction in milk output (up to 50% of daily volume) (KIOSSIS et al, 2013).…”

The investigation of SNP in SOCS2 gene and its effect on milk yield, fat, protein, and somatic cell count in Awassi ewes

“…Following the sampling the blood containers were stored at -20 0 C for later DNA isolation. Genomic DNA was isolated from the whole blood samples by using a Qiagen blood DNA isolation kit.…”