Risk of spread of peneid shrimp viruses in the Americas by the international movement of live and frozen shrimp

Lightner, Donald V.; Redman, Rita M.; Poulos, Bonnie T.; Nunan, Linda M.; Mari, Jocelyne; Hasson, Kenneth W.

doi:10.20506/rst.16.1.1010

Cited by 158 publications

(102 citation statements)

References 30 publications

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“…Hemolymph collected from SPF P. vannamei, which were negative for all of the above viral pathogens, was used as the uninfected control in the dot-immunoblot assays. The disease status of all shrimp was confirmed by showing the presence or absence of characteristic lesions using routine histopathological techniques (Lightner 1996). MAb production.…”

Section: Methodsmentioning

confidence: 99%

Development and application of monoclonal antibodies for the detection of white spot syndrome virus of penaeid shrimp

Poulos¹,

Pantoja²,

Bradley-Dunlop³

et al. 2001

Dis. Aquat. Org.

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Monoclonal antibodies (MAbs) were produced against white spot syndrome virus (WSSV) of penaeid shrimp. The virus isolate used for immunization was obtained from China in 1994 and was passaged in Penaeus vannamei. The 4 hybridomas selected for characterization all produced MAbs that reacted with the 28 kD structural protein by Western blot analysis. The MAbs tested in dot-immunoblot assays were capable of detecting the virus in hemolymph samples collected from moribund shrimp during an experimentally induced WSSV infection. Two of the MAbs were chosen for development of serological detection methods for WSSV. The 2 MAbs detected WSSV infections in fresh tissue impression smears using a fluorescent antibody for final detection. A rapid immunohistochemical method using the MAbs on Davidson's fixed tissue sections identified WSSV-infected cells and tissues in a pattern similar to that seen with digoxigenin-labeled WSSV-specific gene probes. A whole mount assay of pieces of fixed tissue without paraffin embedding and sectioning was also successfully used for detecting the virus. None of the MAbs reacted with hemolymph from specific pathogen-free shrimp or from shrimp infected with infectious hypodermal and hematopoietic necrosis virus, yellow head virus or Taura syndrome virus. In Western blot analysis, the 2 MAbs did not detect any serological differences among WSSV isolates from China, Thailand, India, Texas, South Carolina or Panama. Additionally, the MAbs did not detect a serological difference between WSSV isolated from penaeid shrimp and WSSV isolated from freshwater crayfish. KEY WORDS: White spot syndrome virus · Monoclonal antibodies · ImmunodetectionResale or republication not permitted without written consent of the publisher

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Section: Methodsmentioning

confidence: 99%

Development and application of monoclonal antibodies for the detection of white spot syndrome virus of penaeid shrimp

Poulos¹,

Pantoja²,

Bradley-Dunlop³

et al. 2001

Dis. Aquat. Org.

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“…Serious viral outbreaks often cause catastrophic losses in shrimp farming around the globe. Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is one of the most important DNA viruses infecting penaeid shrimp and the virus causes lethal disease (up to 90% mortality) in Penaeus stylirostris juveniles (Lightner et al, 1996). However, the virus does not cause lethal infection in P. vannamei, instead, it causes reduction in growth and a variety of cuticular deformities of the rostrum, antennae and other thoracic and abdominal areas, deformities that together are commonly known as runt deformity syndrome (Kalagayan et al, 1991).…”

Section: Introductionmentioning

confidence: 99%

Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification

Sun

Ren

et al. 2006

Journal of Virological Methods

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“…The suggestion by some authors [10,11] that the spread could occur through importation of commodity shrimp in international trade has not been scientifically validated [6]. Some examples of such global spread are discussed in this review.…”

Section: Disease Transmission Risk From Shrimps Exported For Human Comentioning

confidence: 89%

“…This supports the view that commodity shrimp in international trade poses negligible or no risk of transmitting shrimp pathogens. It has been argued that imported commodity shrimp may be reprocessed and effluents from such processing plants may pose a threat to natural shrimp populations in an importing country [11]. However, this has not been supported by any scientific evidence.…”

Section: Disease Transmission Risk From Shrimps Exported For Human Comentioning

confidence: 99%

Shrimp Viral Diseases, Import Risk Assessment and International Trade

Karunasagar

Ababouch

2012

Indian J. Virol.

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Shrimp is an important commodity in international trade accounting for 15 % in terms of value of internationally traded seafood products which reached $102.00 billion in 2008. Aquaculture contributes to over 50 % of global shrimp production. One of the major constraints faced by shrimp aquaculture is the loss due to viral diseases like white spot syndrome, yellow head disease, and Taura syndrome. There are several examples of global spread of shrimp diseases due to importation of live shrimp for aquaculture. Though millions of tonnes of frozen or processed shrimp have been traded internationally during the last two decades despite prevalence of viral diseases in shrimp producing areas in Asia and the Americas, there is no evidence of diseases having been transmitted through shrimp imported for human consumption. The guidelines developed by the World Animal Health Organisation for movement of live animals for aquaculture, frozen crustaceans for human consumption, and the regulations implemented by some shrimp importing regions in the world are reviewed.

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Risk of spread of peneid shrimp viruses in the Americas by the international movement of live and frozen shrimp

Cited by 158 publications

References 30 publications

Development and application of monoclonal antibodies for the detection of white spot syndrome virus of penaeid shrimp

Development and application of monoclonal antibodies for the detection of white spot syndrome virus of penaeid shrimp

Sensitive and rapid detection of infectious hypodermal and hematopoietic necrosis virus (IHHNV) in shrimps by loop-mediated isothermal amplification

Shrimp Viral Diseases, Import Risk Assessment and International Trade

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