Risk factors associated with Neospora caninum infections in cattle in Argentina

Moore, D.P.; Pérez, Andrés; Agliano, S.; Brace, M.; Cantón, Germán; Cano, D.; Leunda, María; Odeón, Anselmo Carlos; Odriozola, Ernesto; Campero, Carlos Manuel

doi:10.1016/j.vetpar.2009.01.003

Cited by 46 publications

(40 citation statements)

References 15 publications

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“…Positive cases were more frequent among aborted animals (12.8%) than in animals without history of reproductive problems. This finding is in full agreement with those of Dubey (2003) and Moore et al (2009) who mentioned that aborted animals were more likely to be positive to N. caninum than those without signs of abortion. Romero-Salas et al (2010) reported that N. caninum antibodies prevalence increases with age, a matter that suggests horizontal and trans-placental transmission of the parasite.…”

Section: Discussionsupporting

confidence: 92%

First report of Neospora caninum infection in cattle in Sudan

Ibrahim

Elfahal

Hussein

2011

Trop Anim Health Prod

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A cross-sectional survey was conducted in Sudan to determine sero-prevalence and risk factors associated with Neospora caninum infection in nonvaccinated dairy herds and to assess importance of the disease. Blood samples were collected from a total of 262 animals from 25 herds. Sera were tested for antibodies against N. caninum using ELISA test. The prevalence rates of N. caninum antibodies in cattle were high both at herd level (44%) and at individual animal level (10.7%). Herd level infection rates were similar in Khartoum State (43.7%) and at Gazira States (44.4%). The overall prevalence rates were higher (16.1%) in Gazira State than in Khartoum State (9%) but with no significant variation. The sero-prevalence at individual animal level was significantly higher (p<0.05) in animals with history of abortion (12.8%) than in apparently healthy animal (11.3%), animal with history of infertility (8.1%), or neonatal death of calves (4.3%). In addition, significantly higher (P<0.05) sero-prevalence was observed in samples collected during the rainy season (6.87%) than winter (3.05%) or summer (0.76%). However, no significant differences in sero-prevalence due to locality, animal breed, sex, and age were observed (p > 0.05). This preliminary study reveals for the first time the existence of natural N. caninum infection in Sudan. Also, the findings of the present study indicated that this disease is highly prevalent in two major areas of dairy production in the country, and this calls for control strategy to be implemented.

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Section: Discussionsupporting

confidence: 92%

First report of Neospora caninum infection in cattle in Sudan

Ibrahim

Elfahal

Hussein

2011

Trop Anim Health Prod

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“…The prevalence of N. caninum in infected beef cattle observed during this study was similar to previous descriptions (Moore et al 2002;Bañales et al 2006;Armengol et al 2007;Moore et al 2009), but was lower relative to other reports (Osawa et al 2002;Aguiar et al 2006). The prevalence of N. caninum in dairy and meat cattle from Paraná state (the region where this study was done) was previously described as ranging from 14.31% to 34.8% (Locatelli-Dittrich et al 2001;Ragozo et al 2003;Guimarães et al 2004;Locatelli-Dittrich et al 2008), and 26.7% (Ragozo et al 2003), respectively.…”

Section: Discussionsupporting

confidence: 87%

Neospora caninum: evaluation of vertical transmission in slaughtered beef cows (Bos indicus)

et al. 2010

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Neospora caninum is a protozoan parasite that causes the most important reproductive problems in cattle worldwide. The objective of this study was to evaluate the possibility of vertical transmission of N. caninum in zebus breed beef cows (Bos indicus) submitted for slaughter at an abattoir in the northern region of the State of Paraná, southern Brazil. One hundred and fifty-nine cows were evaluated: 83 pregnant (in different stages of gestation) and 76 non-pregnant. Serum determination of N. caninum was evaluated by indirect ELISA (Idexx). Blood (with EDTA) from pregnant cows and tissue samples (brain and heart) from their fetuses were collected and used for PCR analyses. Antibodies against N. caninum were observed in 14.6% (12/83) of pregnant and in 15.8% (12/76) of non-pregnant cows. Antibodies against the parasites were detected in one fetus (1.4%). The PCR analyses revealed that 6.0% (5/83) of cows and 4.8% (4/83) of fetuses evaluated were positive to specific N. caninum primers. These positive fetuses were between 4 and 6 months of age. Thus, considering PCR and serology as an indicative of vertical transmission in fetuses, 4.8% of fetuses were infected by N. caninum during gestation.

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“…A seroprevalence of 26% in this region was estimated from the results. This value is in agreement with a previous report 24 in which a prevalence of 25% was found for herds with history of reproductive losses in the same area. For concordance, sensitivity, and specificity calculation purposes, the 212 samples were analyzed along with the 16 samples of the experimentally infected heifers.…”

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confidence: 93%

An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle

et al. 2011

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Abstract. Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.

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Risk factors associated with Neospora caninum infections in cattle in Argentina

Cited by 46 publications

References 15 publications

First report of Neospora caninum infection in cattle in Sudan

First report of Neospora caninum infection in cattle in Sudan

Neospora caninum: evaluation of vertical transmission in slaughtered beef cows (Bos indicus)

An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle

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