Risk factors and infection due to Cryptosporidium spp. in dogs and cats in southern Rio Grande do Sul

Moreira, Ângela Nunes; Baptista, Cristiane Telles; Brasil, Carolina Litchina; Valente, Júlia de Souza Silveira; Bruhn, Fábio Raphael Pascoti; Pereira, Daniela Isabel Brayer

doi:10.1590/s1984-296120180012

Cited by 10 publications

(3 citation statements)

References 30 publications

(33 reference statements)

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“…The rate of positive samples is in agreement with other studies conducted in Brazil by only parasitological techniques (Coelho et al, 2009;Funada et al, 2007;Gennari et al, 1999;Huber, Silva, Bomfim, Teixeira, & Bello, 2007;Moreira et al, 2018;Pivoto, Lopes, Vogel, Botton, & Sangioni, 2013;Ragozo et al, 2002). The molecular characterization was performed in just a few studies in Brazil (Alves et al, 2018;Huber et al, 2007;Thomaz, Meireles, Soares, Pena, & Gennari, 2007).…”

Section: Resultssupporting

confidence: 89%

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Neto

Widmer

Lima

et al. 2020

Semina: Ciênc. Agrár.

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We investigated the occurrence of Cryptosporidium oocysts shedding by domestic cats in an urban setting. The calculation of minimum sample size was based on an estimated prevalence of 10%, 5% absolute sampling error and a 5% significance level, resulting in 138 cats. A total of 612 owners of 2,290 cats had to be contacted for achieving the minimal sample size. In the end, only 55 owners accepted to participate in this investigation. Stool samples collected from 138 dogs were examined by microscopy using modified Kinyoun acid-fast staining, capture ELISA and nested-PCR followed by sequencing. Samples were considered positive when Cryptosporidium were detected by at least two diagnostic methods. Thirteen samples were positive (9.4%; 95% CI: 4.5 - 14.3). Cryptosporidium amplicons from seven out of the 13 samples were successfully sequenced and shared 99% genetic similarity to Cryptosporidium felis, GenBank access AF112575.1 was found. We concluded that Cryptosporidium infection is common in domestic cats from urban area and veterinary practitioners should guide cat owners to adopt preventive measures against the parasite to reduce the chance of infection in cats and householders.

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Section: Resultssupporting

confidence: 89%

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Neto

Widmer

Lima

et al. 2020

Semina: Ciênc. Agrár.

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“…Thus far, many studies about the infection of G. duodenalis , Cryptosporidium spp. and E. bieneusi in dogs and cats have been recorded worldwide, such as Asia, Europe and Latin America, although only a few have been reported in Africa ( Table 5 ) [ 6 , 7 , 16 , 20 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 ]. According to the studies in China, the prevalence of G. duodenalis ranges from 4.5–26.2% in dogs and 1.9–13.1% in cats [ 6 , 7 , 25 , 26 ]; the prevalence of Cryptosporidium spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular Investigation of Zoonotic Intestinal Protozoa in Pet Dogs and Cats in Yunnan Province, Southwestern China

Wang

Zou

et al. 2021

Pathogens

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Giardia duodenalis, Enterocytozoon bieneusi and Cryptosporidium spp. are common enteric pathogens that reside in the intestines of humans and animals. These pathogens have a broad host range and worldwide distribution, but are mostly known for their ability to cause diarrhea. However, very limited information on prevalence and genotypes of G. duodenalis, E. bieneusi and Cryptosporidium spp. in pet dogs and cats are available in China. In the present study, a total of 433 fecal samples were collected from 262 pet dogs and 171 pet cats in Yunnan province, southwestern China, and the prevalence and the genotypes of G. duodenalis, E. bieneusi and Cryptosporidium spp. were investigated by nested PCR amplification and DNA sequencing. The prevalence of G. duodenalis, E. bieneusi and Cryptosporidium spp. was 13.7% (36/262), 8.0% (21/262), and 4.6% (12/262) in dogs, and 1.2% (2/171), 0.6% (4/171) and 2.3% (1/171) in cats, respectively. The different living conditions of dogs is a risk factor that is related with the prevalence of G. duodenalis and E. bieneusi (P < 0.05). However, there were no statistically significant difference in prevalence of three pathogens in cats. DNA sequencing and analyses showed that four E. bieneusi genotypes (PtEb IX, CD9, DgEb I and DgEb II), one Cryptosporidium spp. (C. canis) and two G. duodenalis assemblages (C and D) were identified in dogs; two E. bieneusi genotypes (Type IV and CtEb I), one Cryptosporidium spp. (C. felis) and one G. duodenalis assemblage (F) were identified in cats. Three novel E. bieneusi genotypes (DgEb I, DgEb II and CtEb I) were identified, and the human-pathogenic genotypes/species Type IV C. canis and C. felis were also observed in this study, indicating a potential zoonotic threat of pet dogs and cats. Our results revealed the prevalence and genetic diversity of G. duodenalis, E. bieneusi and Cryptosporidium spp. infection in pet dogs and cats in Yunnan province, southwestern China, and suggested the potential threat of pet dogs and cats to public health.

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“…This is supported by a study from Saxony (Germany), in which IIaA15G2R1 was the most prevalent subtype in calves (Holzhausen et al 2019 ). Because of the high prevalence of C. parvum in cattle and broad host spectrum, dogs are probably at a higher risk for an infection when living in areas with cattle farms, as proposed by Moreira et al ( 2018 ) . Cryptosporidium parvum is an important zoonotic agent causing human cryptosporidiosis (Ryan et al 2014 ).…”

Section: Discussionmentioning

confidence: 97%

Cryptosporidium infection in young dogs from Germany

2022

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Cryptosporidium is an enteric protozoan parasite which is able to cause severe gastrointestinal disease and is distributed all over the world. Since information about the prevalence of cryptosporidiosis in German dogs is rare, the aim of this study was to examine the occurrence of Cryptosporidium spp. in dogs and the potential zoonotic risk emanating from these infected animals. In total, 349 fecal samples of 171 dogs were collected during the dogs’ first year of life. The samples were examined for Cryptosporidium spp. using PCR, targeting the small subunit ribosomal RNA gene (SSU rRNA). Further analysis of Cryptosporidium parvum and Cryptosporidium canis positive samples was accomplished using the 60 kDa glycoproteine gene (GP60). Overall, 10.0% (35/349) of the specimens were tested positive for Cryptosporidium. Cryptosporidium canis was found in 94.3% (33/35) of these samples and the zoonotic type C. pavum in 5.7% (2/35). Both C. parvum infections were subtyped as IIaA15G2R1. Sixteen of the C. canis positive samples were successfully amplified at the GP60 gene locus. These isolates were identified to belong to the subtype families XXd, XXe, or XXb; however, 2 samples could not be assigned to any of the described subtype families. Considering the close contact between pets and their owners, dogs may act as a potential source of infection for human cryptosporidiosis. The results of this study, in context with other studies from different countries, provide important further insights into the distribution of Cryptosporidium species in dogs and their zoonotic potential.

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Risk factors and infection due to Cryptosporidium spp. in dogs and cats in southern Rio Grande do Sul

Cited by 10 publications

References 30 publications

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Prevalence of Cryptosporidium infection in domestic cats from an urban area in Brazil

Molecular Investigation of Zoonotic Intestinal Protozoa in Pet Dogs and Cats in Yunnan Province, Southwestern China

Cryptosporidium infection in young dogs from Germany

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