Risk Factors and Co-Existence of Infectious Causes of Reproductive Failures in Selected Uganda Cattle and Goats: A Brucella spps-Toxoplasma gondii Study

Kakooza, Steven; Tumwebaze, Maria Agnes; Nabatta, Esther; Byaruhanga, Joseph; Tayebwa, Dickson Stuart; Wampande, Edward

doi:10.4236/oalib.1104502

Cited by 2 publications

(3 citation statements)

References 13 publications

(18 reference statements)

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“…Brucellosis is one of the most common neglected zoonotic diseases, and it has continued to widely affect the world's population with up to 500,000 new cases worldwide annually in humans, greatly affecting the developing countries such as India, Uganda, Sudan, and among others [1][2][3]. A study conducted in Kampala city in 2011 found out that adjusted herd prevalence of brucellosis was 6.5% (11/177, 95% confidence interval [CI]: 3.6-10.0%) and the adjusted individual animal prevalence was 5.0% (21/423, 95% CI: 2.7-9.3%) based on diagnosis using commercial kits of the competitive enzyme-linked immunosorbent assay (ELISA) for Brucella abortus antibodies [4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Ipola¹,

Kato²,

Ikwap³

et al. 2018

Int J One Health

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Background: In Uganda, detection of brucellosis in goats and humans is more frequently done using rose bengal plate test (RBPT) in comparison to other serological tests such as serum agglutination test (SAT) and indirect enzyme-linked immunosorbent assay (i-ELISA). Aim:This study aimed at estimating the degree of agreement, specificity and sensitivity of RBPT, SAT, and ELISA in brucellosis detection in human and goat samples.Materials and Methods: An analytical study was conducted involving parallel testing of 425 goat samples and 177 human samples to compare RBPT, SAT, and i-ELISA tests of brucellosis detection. Results:A seroprevalence of 14.82% in animals and 11.86% in humans was detected using RBPT, 7.06% in goats and 5.09% in humans using SAT, and 6.35% in humans and 4.52% in animals using i-ELISA. The degree of agreement between the three tests in humans and animals was compared using Fleiss' kappa statistics where a moderate agreement (MA) was obtained for both with kappa value 0.57 in animals and 0.51 in humans and p<0.001. Kappa values in animals were 0.52 (MA) for RBPT and i-ELISA, 0.61 (MA) for RBPT and SAT, and 0.82 substantial agreement (SA) for SAT and i-ELISA. The kappa values in humans were 0.52 (MA) for RBPT and i-ELISA, 0.57 (MA) for RBPT and SAT, and 0.82 (SA) for SAT and i-ELISA, respectively. The study revealed a significant agreement in the tests for all the three tests, but there was SA between SAT and i-ELISA in both animal and human samples. Sensitivity and specificity of RBT and SAT were estimated using i-ELISA as a gold standard as follows, in goats RBPT: 100 and 91% and SAT: 100 and 99%, respectively, and in humans RBPT: 100 and 92% and SAT: 88 and 99%, respectively. Conclusion:It appears that RBPT is good as a screening test, whereas SAT and i-ELISA are good for diagnosis purposes or confirmatory test.

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Section: Introductionmentioning

confidence: 99%

Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Ipola¹,

Kato²,

Ikwap³

et al. 2018

Int J One Health

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“…One of the animal health and zoonotic concerns in Uganda is brucellosis. 8,13 The major zoonotic species are Brucella melitensis , B. abortus , and B. suis , which are transmitted to humans mainly from cattle, sheep, goats, pigs, camels, and wildlife. 14 Brucellosis is a direct zoonosis that is transmitted via fluids or products of diseased animals by direct contact or consumption of contaminated products.…”

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confidence: 99%

“…The direct-contact nature of abattoir work with carcasses ensures that workers are at risk of exposure to Brucella spp. Studies of the prevalence of brucellosis 7,8,13,15 have used various screening tests, such as the rose bengal test (RBT) or ELISA, with fewer trials with molecular and bacterial culture methods. Data evaluating the performance of the tests is scanty in Uganda, which may affect the choice of tests in national disease eradication campaigns, such as the test-and-slaughter approaches that have been effective in the developed world.…”

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Seromonitoring of brucellosis in goats and sheep slaughtered at an abattoir in Kampala, Uganda

et al. 2022

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We conducted a cross-sectional serologic study at Kampala City abattoir in Uganda on 287 small ruminants (221 goats and 66 sheep) to determine the seroprevalence of brucellosis. The samples were tested using a modified rose bengal test (mRBT) and an indirect ELISA (iELISA). Small ruminant Brucella spp. seropositivity was 18 of 287 (6.3%) by mRBT and 19 of 287 (6.6%) by iELISA. The prevalence of brucellosis by mRBT was non-significantly higher in goats (17 of 221; 7.7%) than in sheep (1 of 66, 1.5%; p = 0.069), and also non-significantly higher by the iELISA in goats (18 of 221; 8.1%) than in sheep (1 of 66, 1.5%; p = 0.057). Brucellosis in slaughtered goats and sheep is a public health hazard to abattoir workers and consumers that calls for control and eradication measures at the farm level, given that testing is not carried out routinely at slaughter points.

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Risk Factors and Co-Existence of Infectious Causes of Reproductive Failures in Selected Uganda Cattle and Goats: A Brucella spps-Toxoplasma gondii Study

Cited by 2 publications

References 13 publications

Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Comparison of rose bengal plate test, serum agglutination test, and indirect enzyme-linked immunosorbent assay in brucellosis detection for human and goat samples

Seromonitoring of brucellosis in goats and sheep slaughtered at an abattoir in Kampala, Uganda

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