Risk and clinicopathological features of osteosarcoma metastasis to the lung: A population-based study

Huang, Xiaolun; Zhao, Jian; Bai, Jinyi; Shen, Hua; Zhang, Bingbing; Deng, Lulu; Sun, Chen; Liu, Yanfang; Zhang, Jing; Zheng, Jianming

doi:10.1016/j.jbo.2019.100230

Cited by 89 publications

(99 citation statements)

References 33 publications

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“…Munajat et al found that 33 patients (47%) had evidence of lung metastasis in a cohort of 70 patients with osteosarcoma [15]. Huang et al found that the incidence of lung metastasis was 16.7% of patients with osteosarcoma [16]. In our study, we found osteosarcoma patients with larger tumor size or higher malignancy are risk factors for lung metastases.…”

Section: Discussionsupporting

confidence: 59%

Risk factors for lung metastasis at presentation with malignant primary osseous neoplasms: a population-based study

et al. 2020

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Background: Large population-based studies of risk factor for lung metastases at the presentation with primary osseous neoplasms are lacking and necessary. We aim to examine potential risk factors of lung metastases at presentation with primary osseous neoplasms using Surveillance, Epidemiology, and End Results (SEER) database tool. Methods: We collected patients diagnosed with primary osseous neoplasms between 2010 and 2015 from the SEER database. Patients were divided into two groups: patients with lung metastases or patients without lung metastases. Patient characteristics such as age, sex, race, tumor size, histologic types, histologic grade, and lung metastasis were collected. Univariate and multivariate logistic regression analyses were applied to determine which characteristics were risk factors for lung metastasis at diagnosis. Results: A total of 4459 patients were collected, and 507 patients had lung metastases at presentation. Data on age, race, gender, primary site, grade, tumor size, and histology types were enrolled into the multivariate logistic analysis. Higher grade (OR = 5.197, 95% CI 3.328 to 8.117), histology type (Ewing sarcoma: OR = 1.432, 95% CI 1.020 to 2.009; osteosarcoma: OR = 1.597, 95% CI, 1.073 to 2.377), and larger tumor size (≥ 5 cm: OR = 3.528, 95% CI 2.370 to 5.251) were associated with an increased risk of lung metastasis at presentation. Conclusion: Histology types (osteosarcoma and Ewing sarcoma) were related to a higher risk of lung metastases in primary osseous neoplasms patients. Patients with osteosarcoma and lager tumors or higher tumor grade were associated with higher possibility of lung metastases. Patients with Ewing sarcoma and larger tumors have more tendency of lung metastases. These patients are supposed to receive chest CT scans at the presentation with primary osseous neoplasms.

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Section: Discussionsupporting

confidence: 59%

Risk factors for lung metastasis at presentation with malignant primary osseous neoplasms: a population-based study

et al. 2020

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“…7 These lung-metastasis OS patients have the poorest overall prognosis, with only a 19% five-year survival rate. 8 It is essential to further study the molecular mechanisms associated with OS tumorigenesis, metastasis and drug-resistance to reveal several effective therapeutic options for better management of OS.…”

Section: Introductionmentioning

confidence: 99%

<p>circPVT1 Facilitates Invasion and Metastasis by Regulating miR-205-5p/c-FLIP Axis in Osteosarcoma</p>

Liu

Wan

Lin

et al. 2020

CMAR

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Background: As a key subtype of non-coding RNAs, circular RNA (circRNA) has been well documented to play a key role in the tumorigenesis of osteosarcoma (OS). circPVT1 was revealed to participate in the progression of multiple human tumors; however, the roles of circPVT1 in OS invasion and metastasis and its potential mechanisms remain elusive. Methods: RNA expression in OS tissues and cells was examined by qRT-PCR, protein expression was measured by Western blot. circPVT1 knockdown in vitro was achieved by transfecting OS cells with specific siRNAs. OS cell proliferation was assessed via CCK-8 and colony formation assays. OS cell migration and invasion were evaluated by transwell assay. Interaction between miR-205-5p and circPVT1 or c-FLIP was validated through dualluciferase reporter assay. Rescue experiments were performed to explore the regulatory net among circPVT1, miR-205-5p and c-FLIP in OS progression in vitro. Results: circPVT1 and c-FLIP were highly expressed, while miR-205-5p was lowly expressed in OS tissues and cell lines. Knockdown of circPVT1 repressed cell proliferation, migration and invasion via inhibiting epithelial-mesenchymal transition (EMT) in OS. circPVT1 functioned as a sponge of miR-205-5p, and c-FLIP was targeted by miR-205-5p in OS cells. Furthermore, circPVT1 indirectly regulated c-FLIP expression through competitively binding to miR-205-5p. Inhibition of miR-205-5p or overexpression of c-FLIP abolished the effects of si-circPVT1 on cell proliferation, migration and invasion. Conclusion: Our study demonstrated circPVT1 functions as a sponge for miR-205-5p to promote c-FLIP expression, thereby enhancing EMT and inducing OS invasion and metastasis in vitro, implying that circPVT1 might be a potential therapeutic target for further clinical therapy of OS.

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“…In recent years, due to the development of surgical techniques, multidrug system chemotherapy, precise radiotherapy and immunotherapy, signi cant progress has been made in the treatment of osteosarcoma. However, even with aggressive treatment, the prognosis of patients with lung metastasis remains poor, with a 5-year survival rate of only 20-30% [3,[6][7] . Therefore, it is essential to understand the precise molecular mechanisms involved in lung metastasis of osteosarcoma and thus develop effective diagnostic and therapeutic strategies.…”

Section: Introductionmentioning

confidence: 99%

Identification of key biomarkers and functional pathways in osteosarcomas with lung metastasis: Evidence from bioinformatics analysis

Liu

Zhou

2020

Preprint

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Background: In osteosarcoma, the lung is the most common metastatic organ. Intensive work has been made to illuminate the pathogeny, but the specific metastatic mechanism remains unclear. Thus, we conducted the study to seek to find the key genes and critical functional pathways associated with progression and treatment in osteosarcoma with lung metastasis.Methods: Two independent datasets (GSE14359 and GSE85537) were screened out from the Gene Expression Omnibus (GEO) database and the overlapping differentially expressed genes (DEGs) were identified using GEO2R online platform. Subsequently, the Gene Ontology (GO) annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways enrichment analysis of DEGs were conducted using DAVID. Meanwhile, the protein-protein interaction (PPI) network constructed by STRING was visualized using Cytoscape. Afterwards, the key module and hub genes were extracted from the PPI network using the MCODE and cytoHubba plugin. Moreover, the raw data obtained from GSE73166 and GSE21257 was applied to verify the expression differences and conduct the survival analyses of hub genes, respectively. Finally, the interaction network of miRNAs and hub genes constructed by ENCORI was visualized using Cytoscape.Results: A total of 364 DEGs were identified, comprising 96 downregulated genes and 268 upregulated genes, which were mainly involved in cancer-associated pathways, adherens junction, ECM-receptor interaction, focal adhesion, MAPK signaling pathway. Subsequently, 10 hub genes were obtained and survival analysis demonstrated SKP2 and ASPM were closely related to poor prognosis of patients with osteosarcoma. Finally, hsa-miR-340-5p were found to be most closely associated with these hub genes according to the interaction network of miRNAs and hub genes.Conclusion: The key genes and functional pathways identified in the study may contribute to understanding the molecular mechanisms involved in the carcinogenesis and progression of osteosarcoma with lung metastasis, and provide potential diagnostic and therapeutic targets.

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Risk and clinicopathological features of osteosarcoma metastasis to the lung: A population-based study

Cited by 89 publications

References 33 publications

Risk factors for lung metastasis at presentation with malignant primary osseous neoplasms: a population-based study

Risk factors for lung metastasis at presentation with malignant primary osseous neoplasms: a population-based study

<p>circPVT1 Facilitates Invasion and Metastasis by Regulating miR-205-5p/c-FLIP Axis in Osteosarcoma</p>

Identification of key biomarkers and functional pathways in osteosarcomas with lung metastasis: Evidence from bioinformatics analysis

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