Rising from the Ashes: DNA Repair in Deinococcus radiodurans

Cox, Michael M.; Keck, James L.; Battista, John R.

doi:10.1371/journal.pgen.1000815

Cited by 57 publications

(47 citation statements)

References 16 publications

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“…The genome of D. radiodurans, like those of other bacteria, including E. coli, is highly fragmented after exposure to kGy doses of ionizing radiation (12). However, in contrast to sensitive bacteria, D. radiodurans is able to rejoin these overlapping fragments into complete genomes over a period of 3 to 4 h. After repair, cell division commences normally (6). Thus, radioresistance of D. radiodurans is not due to prevention; instead, this organism relies greatly on a variety of efficient DNA repair functions that have less efficient equivalents in almost all species (12).…”

Section: Discussionmentioning

confidence: 99%

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Bacterial Killing by Dry Metallic Copper Surfaces

Santo

Lam

Elowsky

et al. 2011

Appl Environ Microbiol

457

362

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Metallic copper surfaces rapidly and efficiently kill bacteria. Cells exposed to copper surfaces accumulated large amounts of copper ions, and this copper uptake was faster from dry copper than from moist copper. Cells suffered extensive membrane damage within minutes of exposure to dry copper. Further, cells removed from copper showed loss of cell integrity. Acute contact with metallic copper surfaces did not result in increased mutation rates or DNA lesions. These findings are important first steps for revealing the molecular sensitive targets in cells lethally challenged by exposure to copper surfaces and provide a scientific explanation for the use of copper surfaces as antimicrobial agents for supporting public hygiene.

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Section: Discussionmentioning

confidence: 99%

“…D. radiodurans possesses sophisticated and very effective DNA repair systems enabling cells to recover from stresses resulting in highly fragmented genomes, damage that is lethal to most microbes (6). Nonetheless, stationary-growth-phase cells of D. radiodurans were inactivated after 1 min of exposure (Fig.…”

Section: Methodsmentioning

confidence: 99%

Bacterial Killing by Dry Metallic Copper Surfaces

Santo

Lam

Elowsky

et al. 2011

Appl Environ Microbiol

457

362

View full text Add to dashboard Cite

show abstract

“…Later, it was shown that recF mutants are even more hypersensitive to UV radiation than RecBC, that RecF pathway plays a major role in replication restart under UV damage conditions, and that RecF is involved in DSBs repair in the absence of RecBC and SbcBC (Clark, 1991;Courcelle, 2005;Courcelle et al, 1997;Ivancic-Bace et al, 2003;Kidane et al, 2004;Kusano et al, 1989;Whitby and Lloyd, 1995;Zahradka et al, 2006). Sequencing of new genomes revealed the ubiquitous nature of RecF pathway proteins found in most bacteria (Rocha et al, 2005), including the radiation resistant bacteria Deinococcus radiodurans (Bentchikou et al, 2010;Cox et al, 2010;Chang et al, 2010;Makarova et al, addition to initial loading of RecA, RecOR further stimulate homologous recombination by preventing the dissociation of RecA* filament from ssDNA in E. coli (Bork et al, 2001). Somewhat different properties were reported for Bacillus subtilis RecO, which does not require RecR for initiation of RecA* formation (Manfredi et al, 2008;Manfredi et al, 2010).…”

Section: Recf Pathwaymentioning

confidence: 99%

ATP-Binding Cassette Properties of Recombination Mediator Protein RecF

Korolev¹

2011

DNA Repair

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“…Nonetheless, this observation also implies that biological mutation rates per base-pair are not minimised, since widely varying genome sizes imply very different per-basepair rates. At a mechanistic level, some organisms do exist, such as the bacterium Deinococcus radiodurans with substantially more developed DNA repair or replication mechanisms than closely related species (Cox et al, 2010), implying that mutation rates elsewhere at least are not minimised.…”

Section: Introductionmentioning

confidence: 99%

Theory and Practice of Optimal Mutation Rate Control in Hamming Spaces of DNA Sequences

Belavkin¹,

Channon²,

Aston³

et al. 2011

Ecal 2011

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We investigate the problem of optimal control of mutation by asexual self-replicating organisms represented by points in a metric space. We introduce the notion of a relatively monotonic fitness landscape and consider a generalisation of Fisher's geometric model of adaptation for such spaces. Using a Hamming space as a prime example, we derive the probability of adaptation as a function of reproduction parameters (e.g. mutation size or rate). Optimal control rules for the parameters are derived explicitly for some relatively monotonic landscapes, and then a general information-based heuristic is introduced. We then evaluate our theoretical control functions against optimal mutation functions evolved from a random population of functions using a meta genetic algorithm. Our experimental results show a close match between theory and experiment. We demonstrate this result both in artificial fitness landscapes, defined by a Hamming distance, and a natural landscape, where fitness is defined by a DNA-protein affinity. We discuss how a control of mutation rate could occur and evolve in natural organisms. We also outline future directions of this work.

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Rising from the Ashes: DNA Repair in Deinococcus radiodurans

Cited by 57 publications

References 16 publications

Bacterial Killing by Dry Metallic Copper Surfaces

Bacterial Killing by Dry Metallic Copper Surfaces

ATP-Binding Cassette Properties of Recombination Mediator Protein RecF

Theory and Practice of Optimal Mutation Rate Control in Hamming Spaces of DNA Sequences

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