Rifampin-Releasing Triple-Layer Cross-Linked Fresh Water Fish Collagen Sponges as Wound Dressings

Abstract

Objectives. Surgical wounds resulting from biofilm-producing microorganisms represent a major healthcare problem that requires new and innovative treatment methods. Rifampin is one of a small number of antibiotics that is able to penetrate such biofilms, and its local administration has the potential to serve as an ideal surgical site infection protection and/or treatment agent. This paper presents two types (homogeneous and sandwich structured) of rifampin-releasing carbodiimide-cross-linked fresh water fish collagen wound dressings. Methods. The dressings were prepared by means of the double-lyophilization method and sterilized via gamma irradiation so as to allow for testing in a form that is able to serve for direct clinical use. The mechanical properties were studied via the uniaxial tensile testing method. The in vivo rifampin-release properties were tested by means of a series of incubations in phosphate-buffered saline. The microbiological activity was tested against methicillin-resistant staphylococcus aureus (MRSA) employing disc diffusion tests, and the in vivo pharmacokinetics was tested using a rat model. A histological examination was conducted for the study of the biocompatibility of the dressings. Results. The sandwich-structured dressing demonstrated better mechanical properties due to its exhibiting ability to bear a higher load than the homogeneous sponges, a property that was further improved via the addition of rifampin. The sponges retarded the release of rifampin in vitro, which translated into at least 22 hours of rifampin release in the rat model. This was significantly longer than was achieved via the administration of a subcutaneous rifampin solution. Microbiological activity was proven by the results of the disc diffusion tests. Both sponges exhibited excellent biocompatibility as the cells penetrated into the scaffold, and virtually no signs of local irritation were observed. Conclusions. We present a novel rifampin-releasing sandwich-structured fresh water fish collagen wound dressing that has the potential to serve as an ideal surgical site infection protection and/or treatment agent.

Bacterial cellulose-based scaffold: synthesis and surface modification for sustained rifampicin release for topic skin infections treatment

“…Hartinger et al (Hartinger et al 2020) developed two types (homogeneous and sandwich structured) carbodiimide-cross-linked fresh water sh collagen wound dressings for in vivo use as rifampin-release device and were tested by means of a series of incubations in phosphate-buffered saline. The microbiological activity was tested against methicillin-resistant S. aureus employing disc diffusion tests.…”

“…Perioperative local antibiotic treatment has been widely clinically studied to date with generally promising results (Hartinger et al 2020). Studies suggested that the administration of rifampin by systemic pathway decreases the risk of infections in deep sternal wound infections caused by staphylococci following cardiac surgery (Khanlari et al 2010).…”

“…RIF, is a semisynthetic antibiotic obtained from rifamycin (Sutradhar and Zaman 2021; Hardie and Fenn 2022), used as a rst line treatment for tuberculosis, as well as in the treatment of other infectious diseases (Sutradhar and Zaman 2021) constitute one of the most potent and broad-spectrum antibiotics against others Gram-positive and Gram-negative bacterial pathogens including Staphylococcus epidermidis, Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa (Honig et al 2020), being its antibacterial activity of is due to speci c inhibition of bacterial RNA polymerase (Politano et al 2013;Alifano et al 2014), capable of penetrates readily through the bio lm and the fact that around 80% of surgical site infections are associated with the formation of a poorly permeable bio lm (Edmiston et al 2015;Shiels et al 2018), Skin and surgical wounds infected from bio lm-producing microorganisms, including S. aureus, constitute a major healthcare problem that requires new and innovative treatment methods. Thus, RIF for being able to penetrate bio lms, the local administration has the potential to serve as an ideal surgical site infection protection and / or treatment agent (Hartinger et al 2020).…”

Application of marine collagen for stem‑cell‑based therapy and tissue regeneration (Review)

“…By combining with rifampin, a fish collagen-based wound dressing was produced. This type of wound dressing demonstrated good mechanical properties and exhibited desirable biocompatibility, since the cells penetrated into the sponge and virtually no signs of local irritation were observed ( 88 ). Recently, collagen from the fish scale of Larimichthys crocea was extracted and a hybrid hydrogel based on this collagen was developed for wound healing ( 89 ).…”

Enhancing Guided Bone Regeneration with a Novel Carp Collagen Scaffold: Principles and Applications

“…This secures a favorable environment with sufficient water permeability for cellular processes. Hartinger et al proved that a hydrated sandwich sponge is significantly stiffer than a homogeneous sponge and is capable of withstanding higher loads [107].…”