Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation

Musciacchio, Luigi; Mardirossian, Mario; Guagnini, Benedetta; Raffini, Alessandra; Rizzo, Michele; Trombetta, Carlo; Liguori, Giovanni; Turco, Gianluca; Porrelli, Davide

doi:10.1016/j.matdes.2022.111286

Cited by 6 publications

(4 citation statements)

References 84 publications

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“…The highly porous structure, characterized by open and well-interconnected pores and thin struts within the cells, is probably responsible for this high degradation degree and rate. A further explanation, when comparing the current results with tests carried out in other media, can be ascribed to the better wettability of PCL by DMEM than other mediums like physiological buffer solution (PBS), as determined by Musciacchio et al (2022) [36].…”

Section: Pcl-and Bcp/pcl-based 3d Scaffold Biodegradability Degreesupporting

confidence: 67%

Tuning of Silver Content on the Antibacterial and Biological Properties of Poly(ɛ-caprolactone)/Biphasic Calcium Phosphate 3D-Scaffolds for Bone Tissue Engineering

Menotti,

Scutera,

Coppola

et al. 2023

Polymers

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There is a growing interest in tissue engineering, in which biomaterials play a pivotal role in promoting bone regeneration. Furthermore, smart functionalization can provide biomaterials with the additional role of preventing orthopedic infections. Due to the growing microbial resistance to antimicrobials used to treat those infections, metal ions, such as silver, thanks to their known wide range of bactericidal properties, are believed to be promising additives in developing antibacterial biomaterials. In this work, novel poly(ε-caprolactone) (PCL)-based 3D scaffolds have been designed and developed, where the polymer matrix was modified with both silver (Ag), to supply antibacterial behavior, and calcium phosphates (biphasic calcium phosphate, BCP) particles to impart bioactive/bioresorbable properties. The microstructural analysis showed that constructs were characterized by square-shaped macropores, in line with the morphology and size of the templating salts used as pore formers. Degradation tests demonstrated the important role of calcium phosphates in improving PCL hydrophilicity, leading to a higher degradation degree for BCP/PCL composites compared to the neat polymer after 18 days of soaking. The appearance of an inhibition halo around the silver-functionalized PCL scaffolds for assayed microorganisms and a significant (p < 0.05) decrease in both adherent and planktonic bacteria demonstrate the Ag+ release from the 3D constructs. Furthermore, the PCL scaffolds enriched with the lowest silver percentages did not hamper the viability and proliferation of Saos-2 cells. A synergic combination of antimicrobial, osteoproliferative and biodegradable features provided to 3D scaffolds the required potential for bone tissue engineering, beside anti-microbial properties for reduction in prosthetic joints infections.

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Section: Pcl-and Bcp/pcl-based 3d Scaffold Biodegradability Degreesupporting

confidence: 67%

Tuning of Silver Content on the Antibacterial and Biological Properties of Poly(ɛ-caprolactone)/Biphasic Calcium Phosphate 3D-Scaffolds for Bone Tissue Engineering

Menotti,

Scutera,

Coppola

et al. 2023

Polymers

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“…The effect of the collector speed on fiber orientation and inner diameters of coaxial electrospun hollow fibers was assessed statistically by firstly determining the distribution of the data set using the Kolmogorov-Smirnov test. 39 The data set was then assessed for homogenous vari- is consistent with the observation of their surface morphology (Figure 2B). For the 1.4 mL/h strip, fiber merging, as shown by crosssectional SEM (Figure 2K), was also similar to that shown by its surface topography (Figure 2E).…”

Section: Discussionmentioning

confidence: 83%

“…The effect of the collector speed on fiber orientation and inner diameters of coaxial electrospun hollow fibers was assessed statistically by firstly determining the distribution of the data set using the Kolmogorov–Smirnov test. 39 The data set was then assessed for homogenous variance using Levene's test. Data sets which fulfilled the Kolmogorov–Smirnov test and Levene's test criteria were assessed using ANOVA with a Tukey post‐test.…”

Section: Methodsmentioning

confidence: 99%

Direct jet coaxial electrospinning of axon‐mimicking fibers for diffusion tensor imaging

Grech‐Sollars

Statton

et al. 2023

Polymers for Advanced Techs

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Hollow polymer microfibers with variable microstructural and hydrophilic properties were proposed as building elements to create axon‐mimicking phantoms for validation of diffusion tensor imaging (DTI). The axon‐mimicking microfibers were fabricated in a mm‐thick 3D anisotropic fiber strip, by direct jet coaxial electrospinning of PCL/polysiloxane‐based surfactant (PSi) mixture as shell and polyethylene oxide (PEO) as core. Hydrophilic PCL‐PSi fiber strips were first obtained by carefully selecting appropriate solvents for the core and appropriate fiber collector rotating and transverse speeds. The porous cross‐section and anisotropic orientation of axon‐mimicking fibers were then quantitatively evaluated using two ImageJ plugins—nearest distance (ND) and directionality based on their scanning electron microscopy (SEM) images. Third, axon‐mimicking phantom was constructed from PCL‐PSi fiber strips with variable porous‐section and fiber orientation and tested on a 3T clinical MR scanner. The relationship between DTI measurements (mean diffusivity [MD] and fractional anisotropy [FA]) of phantom samples and their pore size and fiber orientation was investigated. Two key microstructural parameters of axon‐mimicking phantoms including normalized pore distance and dispersion of fiber orientation could well interpret the variations in DTI measurements. Two PCL‐PSi phantom samples made from different regions of the same fiber strips were found to have similar MD and FA values, indicating that the direct jet coaxial electrospun fiber strips had consistent microstructure. More importantly, the MD and FA values of the developed axon‐mimicking phantoms were mostly in the biologically relevant range.

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“…The hydrophobic nature of the PCL/Rif membrane allowed for moistening by physiological liquids and cell media, ensuring cell adhesion, proliferation, and prolonged antibacterial activity against urothelial cells and smooth muscle cells, making it suitable for ureteral regeneration. This fibrous membrane stands as a potential ureteral stent for urothelial carcinoma [ 153 ].…”

Section: Application Of Drug-loaded Nanofibers In Cancer Therapymentioning

confidence: 99%

Application of Electrospun Drug-Loaded Nanofibers in Cancer Therapy

Yang,

Zhang,

Liang

et al. 2024

Polymers

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In the 21st century, chemotherapy stands as a primary treatment method for prevalent diseases, yet drug resistance remains a pressing challenge. Utilizing electrospinning to support chemotherapy drugs offers sustained and controlled release methods in contrast to oral and implantable drug delivery modes, which enable localized treatment of distinct tumor types. Moreover, the core–sheath structure in electrospinning bears advantages in dual-drug loading: the core and sheath layers can carry different drugs, facilitating collaborative treatment to counter chemotherapy drug resistance. This approach minimizes patient discomfort associated with multiple-drug administration. Electrospun fibers not only transport drugs but can also integrate metal particles and targeted compounds, enabling combinations of chemotherapy with magnetic and heat therapies for comprehensive cancer treatment. This review delves into electrospinning preparation techniques and drug delivery methods tailored to various cancers, foreseeing their promising roles in cancer treatment.

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Rifampicin-loaded electrospun polycaprolactone membranes: Characterization of stability, antibacterial effects and urotheliocytes proliferation

Cited by 6 publications

References 84 publications

Tuning of Silver Content on the Antibacterial and Biological Properties of Poly(ɛ-caprolactone)/Biphasic Calcium Phosphate 3D-Scaffolds for Bone Tissue Engineering

Tuning of Silver Content on the Antibacterial and Biological Properties of Poly(ɛ-caprolactone)/Biphasic Calcium Phosphate 3D-Scaffolds for Bone Tissue Engineering

Direct jet coaxial electrospinning of axon‐mimicking fibers for diffusion tensor imaging

Application of Electrospun Drug-Loaded Nanofibers in Cancer Therapy

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