Rice MEDIATOR25, OsMED25, is an essential subunit for jasmonate-mediated root development and OsMYC2-mediated leaf senescence

Suzuki, Go; Lucob-Agustin, Nonawin; Kashihara, Keita; Fujii, Yumi; Inukai, Yoshiaki; Gomi, Kenji

doi:10.1016/j.plantsci.2021.110853

Cited by 16 publications

(12 citation statements)

References 49 publications

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“…As part of general transcriptional regulation, the Mediator complex subunits connect specific transcriptional activators to the RNA polymerase II complex, and some of the individual Mediator complex subunits receive signals from specific pathways and transfer them to general transcriptional mechanisms. Some Mediator complex subunits have been shown to be related to defense, such as Med21 and Med25 [ 14 , 55 , 56 , 57 , 58 , 59 ]. Moreover, in A. thaliana , Med16 was not only proven to regulate the immune response but also proven to be an important subunit in the tail module, as the whole tail module was missing after extraction of the mediator complex in atsrf6 mutants.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of OsMed16 Inhibits the Growth of Rice and Causes Spontaneous Cell Death

Jiang

Yang

Xin

et al. 2021

Genes

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The Mediator complex transduces information from the DNA-bound transcription factors to the RNA polymerase II transcriptional machinery. Research on plant Mediator subunits has primarily been performed in Arabidopsis, while very few of them have been functionally characterized in rice. In this study, the rice Mediator subunit 16, OsMed16, was examined. OsMed16 encodes a putative protein of 1301 amino acids, which is longer than the version previously reported. It was expressed in various rice organs and localized to the nucleus. The knockout of OsMed16 resulted in rice seedling lethality. Its overexpression led to the retardation of rice growth, low yield, and spontaneous cell death in the leaf blade and sheath. RNA sequencing suggested that the overexpression of OsMed16 altered the expression of a large number of genes. Among them, the upregulation of some defense-related genes was verified. OsMed16 can regulate the expression of a wealth of genes, and alterations in its expression have a profound impact on plant growth, development, and defense responses in rice.

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Section: Discussionmentioning

confidence: 99%

Overexpression of OsMed16 Inhibits the Growth of Rice and Causes Spontaneous Cell Death

Jiang

Yang

Xin

et al. 2021

Genes

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“…Since we previously reported that OsMED25 is involved in root development and leaf senescence in rice through JA signaling [30], in the present study we investigated whether OsMED25 is involved in Xoo resistance. The osmed25 mutants were inoculated with virulent Xoo, and lengths of blight lesions were measured two weeks after the inoculation.…”

Section: Analysis Of Xoo Resistance In Osmed25 Mutantsmentioning

confidence: 99%

“…Because OsMED25 positively affected OsMYC2-mediated JA signaling in rice [30], we further investigated the expression of previously reported OsMYC2-responsive defenserelated genes [17] in the osmed25 mutants, which include resistance protein (Os03g03500), lipid transfer protein (Os10g08780), peroxidase (OsPrx112; Os07g48030), lipid transfer protein (Os10g36100), lipid transfer protein (Os10g40420), beta-1,4-glucanase (Os02g50040), thaumatinlike protein (Os10g05600), and beta-1,3-glucanase (Os01g58730). All the OsMYC2-responsive defense-related genes examined, except beta-1,4-glucanase (Os02g50040), tended to be downregulated in osmed25 mutants compared to WT plants (Figure 2a).…”

Section: Expression Of Ja-responsive Defense-related Genes In Osmed25...mentioning

confidence: 99%

“…The MEDIATOR25 (MED25) subunit was first isolated as a regulator involved in the flowering process, and it also plays important roles in other plant growth processes regulated by several plant hormones, including JA [26,28]. Recently, we reported that osmed25 loss-of-function rice mutants exhibited a JA-insensitive phenotype, and OsMED25 acts as a positive regulator in OsMYC2-mediated leaf senescence [29,30]. The OsMED25 directly interacts with OsMYC2 and positively regulates OsMYC2-responsive senescence-associated genes [30].…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we reported that osmed25 loss-of-function rice mutants exhibited a JA-insensitive phenotype, and OsMED25 acts as a positive regulator in OsMYC2-mediated leaf senescence [29,30]. The OsMED25 directly interacts with OsMYC2 and positively regulates OsMYC2-responsive senescence-associated genes [30].…”

Section: Introductionmentioning

confidence: 99%

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The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Suzuki

Fukuda

Lucob-Agustin

et al. 2022

Plants

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Rice bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most severe diseases of rice. However, the regulatory mechanisms of rice defense against Xoo remain poorly understood. The rice MEDIATOR25, OsMED25—a subunit of the mediator multiprotein complex that acts as a universal adaptor between transcription factors (TFs) and RNA polymerase II—plays an important role in jasmonic acid (JA)-mediated lateral root development in rice. In this study, we found that OsMED25 also plays an important role in JA- and auxin-mediated resistance responses against rice bacterial blight. The osmed25 loss-of-function mutant exhibited high resistance to Xoo. The expression of JA-responsive defense-related genes regulated by OsMYC2, which is a positive TF in JA signaling, was downregulated in osmed25 mutants. Conversely, expression of some OsMYC2-independent JA-responsive defense-related genes was upregulated in osmed25 mutants. Furthermore, OsMED25 interacted with some AUXIN RESPONSE FACTORS (OsARFs) that regulate auxin signaling, whereas the mutated osmed25 protein did not interact with the OsARFs. The expression of auxin-responsive genes was downregulated in osmed25 mutants, and auxin-induced susceptibility to Xoo was not observed in osmed25 mutants. These results indicate that OsMED25 plays an important role in the stable regulation of JA- and auxin-mediated signaling in rice defense response.

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Overexpression of OsNINJA1-interacting protein OsFHA1 affects jasmonate-mediated OsMYC2 signaling but not disease resistance in rice

2022

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Rice MEDIATOR25, OsMED25, is an essential subunit for jasmonate-mediated root development and OsMYC2-mediated leaf senescence

Cited by 16 publications

References 49 publications

Overexpression of OsMed16 Inhibits the Growth of Rice and Causes Spontaneous Cell Death

Overexpression of OsMed16 Inhibits the Growth of Rice and Causes Spontaneous Cell Death

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Overexpression of OsNINJA1-interacting protein OsFHA1 affects jasmonate-mediated OsMYC2 signaling but not disease resistance in rice

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