Overexpression of OsNINJA1-interacting protein OsFHA1 affects jasmonate-mediated OsMYC2 signaling but not disease resistance in rice

Kashihara, Keita; Ojiri, Tomomi; Gomi, Kenji

doi:10.1007/s10327-022-01069-2

Cited by 4 publications

(3 citation statements)

References 35 publications

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“…In our previous studies, we consistently observed that JA exerts beneficial effects regarding Xoo resistance in rice [5,7,[12][13][14][15][16][17][18][23][24][25]37]. In the present study, however, we observed that the JA-insensitive osmed25 mutants exhibited strong resistance to Xoo.…”

Section: Discussionsupporting

confidence: 42%

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Suzuki

Fukuda

Lucob-Agustin

et al. 2022

Plants

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Rice bacterial blight disease caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most severe diseases of rice. However, the regulatory mechanisms of rice defense against Xoo remain poorly understood. The rice MEDIATOR25, OsMED25—a subunit of the mediator multiprotein complex that acts as a universal adaptor between transcription factors (TFs) and RNA polymerase II—plays an important role in jasmonic acid (JA)-mediated lateral root development in rice. In this study, we found that OsMED25 also plays an important role in JA- and auxin-mediated resistance responses against rice bacterial blight. The osmed25 loss-of-function mutant exhibited high resistance to Xoo. The expression of JA-responsive defense-related genes regulated by OsMYC2, which is a positive TF in JA signaling, was downregulated in osmed25 mutants. Conversely, expression of some OsMYC2-independent JA-responsive defense-related genes was upregulated in osmed25 mutants. Furthermore, OsMED25 interacted with some AUXIN RESPONSE FACTORS (OsARFs) that regulate auxin signaling, whereas the mutated osmed25 protein did not interact with the OsARFs. The expression of auxin-responsive genes was downregulated in osmed25 mutants, and auxin-induced susceptibility to Xoo was not observed in osmed25 mutants. These results indicate that OsMED25 plays an important role in the stable regulation of JA- and auxin-mediated signaling in rice defense response.

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Section: Discussionsupporting

confidence: 42%

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Suzuki

Fukuda

Lucob-Agustin

et al. 2022

Plants

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“…Rice NOVEL INTERACTOR OF JAZ (OsNINJA1) negatively regulates expression of the OsMYC2‐dependent defence‐related genes by forming a complex with JAZ proteins, and transgenic rice plants overexpressing OsNINJA1 are more susceptible to Xoo (Kashihara et al, 2019). The OsNINJA1‐interacting proteins, SIMILAR TO RCD ONE1a (OsSRO1a) and forkhead‐associated (FHA) domain‐containing protein1 (OsFHA1), also act as negative regulators in the OsMYC2‐mediated defence response in rice (Kashihara et al, 2020; Kashihara et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Jasmonic acid (JA)‐mediating MYB transcription factor1, JMTF1, coordinates the balance between JA and auxin signalling in the rice defence response

Uji,

Suzuki,

Fujii

et al. 2024

Physiologia Plantarum

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The plant hormone jasmonic acid (JA) is a signalling compound involved in the regulation of cellular defence and development in plants. In this study, we investigated the roles of a JA‐responsive MYB transcription factor, JMTF1, in the JA‐regulated defence response against rice bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo). JMTF1 did not interact with any JASMONATE ZIM‐domain (JAZ) proteins. Transgenic rice plants overexpressing JMTF1 showed a JA‐hypersensitive phenotype and enhanced resistance against Xoo. JMTF1 upregulated the expression of a peroxidase, OsPrx26, and monoterpene synthase, OsTPS24, which are involved in the biosynthesis of lignin and antibacterial monoterpene, γ‐terpinene, respectively. OsPrx26 was mainly expressed in the vascular bundle. Transgenic rice plants overexpressing OsPrx26 showed enhanced resistance against Xoo. In addition to the JA‐hypersensitive phenotype, the JMTF1‐overexpressing rice plants showed a typical auxin‐related phenotype. The leaf divergence and shoot gravitropic responses were defective, and the number of lateral roots decreased significantly in the JMTF1‐overexpressing rice plants. JMTF1 downregulated the expression of auxin‐responsive genes but upregulated the expression of OsIAA13, a suppressor of auxin signalling. The rice gain‐of‐function mutant Osiaa13 showed high resistance against Xoo. Transgenic rice plants overexpressing OsEXPA4, a JMTF1‐downregulated auxin‐responsive gene, showed increased susceptibility to Xoo. JMTF1 is selectively bound to the promoter of OsPrx26 in vivo. These results suggest that JMTF1 positively regulates disease resistance against Xoo by coordinating crosstalk between JA‐ and auxin‐signalling in rice.

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“…Transgenic rice plants overexpressing the C-terminal-deleted OsJAZ8ΔC exhibit a JA-insensitive phenotype and decreased JA-induced resistance against Xoo [ 10 ]. Moreover, overexpression of OsNINJA1 and its interactors, OsSRO1a and OsFHA1 , results in the suppression of OsMYC2-dependent defense responses in rice [ 8 , 11 , 12 ]. Additionally, a WRKY-type TF, OsWRKY72, directly binds to the promoter of the rice JA biosynthesis gene, OsAOS1 , and represses its transcription, resulting in the reduction of endogenous JA levels and an increase in Xoo susceptibility in rice [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Jasmonic Acid-Induced β-Cyclocitral Confers Resistance to Bacterial Blight and Negatively Affects Abscisic Acid Biosynthesis in Rice

Taniguchi¹,

Takeda²,

Kiryu³

et al. 2023

IJMS

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Jasmonic acid (JA) regulates the production of several plant volatiles that are involved in plant defense mechanisms. In this study, we report that the JA-responsive volatile apocarotenoid, β-cyclocitral (β-cyc), negatively affects abscisic acid (ABA) biosynthesis and induces a defense response against Xanthomonas oryzae pv. oryzae (Xoo), which causes bacterial blight in rice (Oryza sativa L.). JA-induced accumulation of β-cyc was regulated by OsJAZ8, a repressor of JA signaling in rice. Treatment with β-cyc induced resistance against Xoo and upregulated the expression of defense-related genes in rice. Conversely, the expression of ABA-responsive genes, including ABA-biosynthesis genes, was downregulated by JA and β-cyc treatment, resulting in a decrease in ABA levels in rice. β-cyc did not inhibit the ABA-dependent interactions between OsPYL/RCAR5 and OsPP2C49 in yeast cells. Furthermore, we revealed that JA-responsive rice carotenoid cleavage dioxygenase 4b (OsCCD4b) was localized in the chloroplast and produced β-cyc both in vitro and in planta. These results suggest that β-cyc plays an important role in the JA-mediated resistance against Xoo in rice.

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Overexpression of OsNINJA1-interacting protein OsFHA1 affects jasmonate-mediated OsMYC2 signaling but not disease resistance in rice

Cited by 4 publications

References 35 publications

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

The Mutation of Rice MEDIATOR25, OsMED25, Induces Rice Bacterial Blight Resistance through Altering Jasmonate- and Auxin-Signaling

Jasmonic acid (JA)‐mediating MYB transcription factor1, JMTF1, coordinates the balance between JA and auxin signalling in the rice defence response

Jasmonic Acid-Induced β-Cyclocitral Confers Resistance to Bacterial Blight and Negatively Affects Abscisic Acid Biosynthesis in Rice

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