Rice chloroplast DNA: a physical map and the location of the genes for the large subunit of ribulose 1,5-bisphosphate carboxylase and the 32 KD photosystem II reaction center protein

Hirai, Atsushi; Ishibashi, Takashi; Morikami, Atsushi; Iwatsuki, Norio; Shinozaki, Kazuo; Sugiura, Masahiro

doi:10.1007/bf00275309

Cited by 80 publications

(18 citation statements)

References 10 publications

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“…The DIG‐labelled DNA probes for rbcS , rbcL and glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) were prepared from fragments of rice rbcS (Matsuoka et al . 1988), rbcL (Hirai et al . 1985) and GAPDH gene (GenBank accession no. )…”

Section: Methodsmentioning

confidence: 99%

Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1·5‐bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence

Imai

Suzuki

Makino

et al. 2005

Plant Cell & Environment

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Soon after the emergence of the eighth leaf blades, rice plants were grown with two (1 and 4 m M ) levels of nitrogen (N) supply, and the relationships between the levels of rbcS and rbcL mRNAs, the amount of ribulose 1·5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39) synthesized and the N influx were examined in the eighth leaf blades from emergence through senescence. The levels of both rbcS and rbcL mRNAs, the amount of Rubisco synthesized and the N influx were greater for the 4 m M N treatment than for the 1 m M N treatment throughout the experiment. The amount of Rubisco synthesized was well correlated with the levels of both mRNAs during leaf expansion, but not after the completion of leaf expansion in both N treatments. The ratio of the amount of Rubisco synthesized to the levels of both mRNAs dramatically declined after full expansion. On the other hand, the amount of Rubisco synthesized was well correlated with the N influx in both N treatments. These results indicate that the N influx, namely, N availability, rather than the levels of rbcS and rbcL mRNAs, is more closely related to the amount of Rubisco synthesized in the leaf blade of rice throughout the lifespan of a leaf.

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Section: Methodsmentioning

confidence: 99%

Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1·5‐bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence

Imai

Suzuki

Makino

et al. 2005

Plant Cell & Environment

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show abstract

“…The entire rice chloroplast DNA sequence was determined by the dideoxy chain termination method using a clone bank of overlapping fragments (Hirai et al 1985). By virtue of recombination between the two IRs, chloroplast genomes exist as equimolar mixtures of two isomers differing from each other in the relative orientation of their two single copy regions ).…”

Section: Conserved Genesmentioning

confidence: 99%

The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals

et al. 1989

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The entire chloroplast genome of the monocot rice (Oryza sativa) has been sequenced and comprises 134525 bp. Predicted genes have been identified along with open reading frames (ORFs) conserved between rice and the previously sequenced chloroplast genomes, a dicot, tobacco (Nicotiana tabacum), and a liverwort (Marchantia polymorpha). The same complement of 30 tRNA and 4 rRNA genes has been conserved between rice and tobacco. Most ORFs extensively conserved between N. tabacum and M. polymorpha are also conserved intact in rice. However, several such ORFs are entirely absent in rice, or present only in severely truncated form. Structural changes are also apparent in the genome relative to tobacco. The inverted repeats, characteristic of chloroplast genome structure, have expanded outward to include several genes present only once per genome in tobacco and liverwort and the large single copy region has undergone a series of inversions which predate the divergence of the cereals. A chimeric tRNA pseudogene overlaps an apparent endpoint of the largest inversion, and a model invoking illegitimate recombination between tRNA genes is proposed which accounts simultaneously for the origin of this pseudogene, the large inversion and the creation of repeated sequences near the inversion endpoints.

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“…Chloroplast DNA was isolated from 12-day-old, light-grown seedlings by the method described by Hirai et al (1985), with the addition of CsC1-bisbenzamide gradient purification. The ctDNAs were vacuum dried and dissolved in TE buffer.…”

Section: Methodsmentioning

confidence: 99%

Mitochondrial DNA rearrangements in Pennisetum associated with reversion from cytoplasmic male sterility to fertility

1987

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Endonuclease restriction fragment patterns of Pennisetum americanum L. mitochondrial DNAs (mtDNAs) from a cytoplasmic male-sterile (CMS-A1), fertile revertants and a normal fertile cytoplasm were variable, while chloroplast DNA from those lines lacked variation. Comparisons between mtDNAs of CMS-A1 (parental) and fertile revertant lines revealed the presence of a unique 4.7 kbp PstI fragment in the sterile line that was not detected in any of the revertant lines. A 9.7 kbp PstI fragment was found in all of the revertants, but not in the CMS-A1. Neither of those fragments was found in the normal cytoplasm mtDNA. Hybridization studies revealed two sets of multiple homologies: 1) the 4.7 kbp fragment had homology with a 10.9 kbp and a 13.6 kbp fragment; and 2) the 9.7 kbp fragment was homologous with the 13.6 kbp fragment. The presence of those two repeated mitochondrial sequences on the altered fragments suggests that they may be involved in the recombinational associated events with reversion from CMS to fertility in P. americanum.

show abstract

Rice chloroplast DNA: a physical map and the location of the genes for the large subunit of ribulose 1,5-bisphosphate carboxylase and the 32 KD photosystem II reaction center protein

Cited by 80 publications

References 10 publications

Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1·5‐bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence

Effects of nitrogen nutrition on the relationships between the levels of rbcS and rbcL mRNAs and the amount of ribulose 1·5‐bisphosphate carboxylase/oxygenase synthesized in the eighth leaves of rice from emergence through senescence

The complete sequence of the rice (Oryza sativa) chloroplast genome: Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals

Mitochondrial DNA rearrangements in Pennisetum associated with reversion from cytoplasmic male sterility to fertility

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