Rice bran lipase catalyzed esterification of palm oil fatty acid distillate and glycerol in organic solvent

Chong, Fui Chin; Tey, Beng Ti; Dom, Zanariah Mohd; Cheong, Kok Hing; Satiawihardja, Budiatman; Ibrahim, Mohd Nordin; Rahman, Russly Abdul; Biak, Dayang Radiah Awang; Ling, Tau Chuan

doi:10.1007/bf02931072

Cited by 5 publications

(6 citation statements)

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“…Within cereal seeds, the lipases from rice, wheat, barley, oat, and maize have been tested for their use as biocatalysts, and most of these are resistant to high temperatures and alkali conditions [ 81 , 82 ]. Interestingly, Chong and coworkers [ 83 ] successfully developed a method for the biosynthesis of acylglycerols using a delipidated rice bran lipase to esterify palm oil FAs with glycerol. In recent years, the industrial applications of lipases obtained from latex from different rubber trees have been exploited [ 25 ].…”

Section: Lipase and Phospholipase In Biocatalysismentioning

confidence: 99%

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Borrelli

Trono

2015

IJMS

275

190

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Lipases and phospholipases are interfacial enzymes that hydrolyze hydrophobic ester linkages of triacylglycerols and phospholipids, respectively. In addition to their role as esterases, these enzymes catalyze a plethora of other reactions; indeed, lipases also catalyze esterification, transesterification and interesterification reactions, and phospholipases also show acyltransferase, transacylase and transphosphatidylation activities. Thus, lipases and phospholipases represent versatile biocatalysts that are widely used in various industrial applications, such as for biodiesels, food, nutraceuticals, oil degumming and detergents; minor applications also include bioremediation, agriculture, cosmetics, leather and paper industries. These enzymes are ubiquitous in most living organisms, across animals, plants, yeasts, fungi and bacteria. For their greater availability and their ease of production, microbial lipases and phospholipases are preferred to those derived from animals and plants. Nevertheless, traditional purification strategies from microbe cultures have a number of disadvantages, which include non-reproducibility and low yields. Moreover, native microbial enzymes are not always suitable for biocatalytic processes. The development of molecular techniques for the production of recombinant heterologous proteins in a host system has overcome these constraints, as this allows high-level protein expression and production of new redesigned enzymes with improved catalytic properties. These can meet the requirements of specific industrial process better than the native enzymes. The purpose of this review is to give an overview of the structural and functional features of lipases and phospholipases, to describe the recent advances in optimization of the production of recombinant lipases and phospholipases, and to summarize the information available relating to their major applications in industrial processes.

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Section: Lipase and Phospholipase In Biocatalysismentioning

confidence: 99%

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Borrelli

Trono

2015

IJMS

275

190

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“…Sintesis gliserida dengan memanfaatkan PFAD sebagai sumber asam lemak untuk menjadi mono dan diasilgliserol telah dilaporkan oleh Chong et al, 2007;Dom et al, 2009;Santisawadi et al, 2013;Rumondang et al, 2016dan Setyaningsih et al, 2016. Selain mono dan diasilgliserol sebagai produk utama dalam gliserolisis PFAD juga akan terbentuk triasilgliserol namun tidak banyak yang mengkaji senyawa triasilgliserol yang terbentuk tersebut.…”

Section: Pendahuluanunclassified

Enzimatik Esterifikasi Menggunakan Lipase Antara Asam Lemak Sawit Destilat Dan Gliserol Untuk Sintesis Triasilgliserol

Hasibuan

Ijah

2018

J.Wart.Industri.Has.Pertan.

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Asam lemak sawit destilat (palm fatty acid distillate,PFAD) merupakan fraksi asam lemak bebas dari hasil rafinasi minyak sawit mentah. Penelitian ini dilakukan untuk mengkaji esterifikasi antara PFAD dan gliserol untuk sintesis triasilgliserol secara enzimatik menggunakan enzim lipase sebagai katalis. Rasio mol antara gliserol dan PFAD yang digunakan adalah 1:1-1:10, waktu reaksi selama 4-72 jam, suhu reaksi 50-80 °C dan jumlah katalis lipase sebanyak 1-20 %. Produk esterifikasi dianalisa kadar monoasilgliserol, diasilgliserol, triasilgliserol dan asam lemak bebas. Senyawa triasilgliserol dikarakterisasi kandungan triasilgliserol. Hasil penelitian menunjukkan bahwa senyawa triasilgliserol dapat diperoleh dengan kadar triasilgliserol sebesar 21-24 % pada rasio gliserol dan PFAD 1/6, waktu reaksi selama 72 jam, suhu reaksi 60 °C dan katalis lipase sebanyak 1 %. Senyawa triasilgliserol memiliki kandungan 1,2,3-tripalmitoylglycerol (PPP) 19,4 %, 1,3-dipalmitoyl-2-oleoyl-glycerol (POP) 32,4 %, dan 1(3)palmitoyl-2,3(1)-dioleyl-glycerol (POO) 17,7 %.

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“…Plant lipase has been produced from Jatropha seeds (Abigor, 2002;Hidayat et al, 2014), palm oil (Ebongue et al, 2006), and rice bran (Chong et al, 2007;Hidayat et al, 2014). Bacterial lipases have been obtained from Bacillus coagulans and Pseudomonas aeruginosa (Alkan, 2007;Mahanta et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

et al. 2017

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Jatropha curcas seed cake contains a high amount of protein, and consequently has very high potential as a medium for lipase production. The objective of this research was to characterize lipase from Aspergillus niger 6516, which was produced by solid-state fermentation on Jatropha curcas seed cake as the medium. The effects of pH and temperature on enzyme activity were evaluated, along with substrate specificity and enzyme stability. Fermentation was performed at a water concentration of 63% and temperature of 30 °C for 7 days. The results showed that the optimum pH and temperature for Aspergillus niger 6516 lipase activities were 8.0 and 40 °C, respectively. The lipase had the substrate specificity to hydrolyze long-chain fatty acids and was stable in polar organic solvents. The lipase had a molecular weight, Km and v max about 19 kDa, 0.27 µmol/ml, and 52.63 µmol/ml/min, respectively. The results also suggested that the produced lipase from Aspergillus niger 6516 was an alkaline lipase. Based on these results, we conclude that Jatropha seed cake is a suitable medium for lipase production.

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Rice bran lipase catalyzed esterification of palm oil fatty acid distillate and glycerol in organic solvent

Cited by 5 publications

References 0 publications

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Recombinant Lipases and Phospholipases and Their Use as Biocatalysts for Industrial Applications

Enzimatik Esterifikasi Menggunakan Lipase Antara Asam Lemak Sawit Destilat Dan Gliserol Untuk Sintesis Triasilgliserol

Characterization of Aspergillus Niger 65i6 lipase from solid-state fermentation using Jatropha seed cake medium

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