Ribotype Delineation and Description of Staphylococcus sciuri Subspecies and Their Potential as Reservoirs of Methicillin Resistance and Staphylolytic Enzyme Genes

Kloos, Wesley E.; Ballard, Deborah N.; Webster, John; Hubner, Romero J.; Tomasz, Alexander; Couto, Isabel; Sloan, Gary L.; Dehart, Heather P.; Fiedler, F.; Schubert, K.; Lencastre, Hermı́nia de; Sanches, Ilda Santos; Heath, Harry E.; Leblanc, P; Ljungh, A.

doi:10.1099/00207713-47-4-1279

Cited by 8 publications

(22 citation statements)

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“…Staphylococcus sciuri , the coagulase‐negative species, was first described by Kloos et al in 1976 [1]. This bacterium is widespread in nature and is associated with a variety of wild and domestic animals [1–4]. S. sciuri has been shown to be an invasive pathogen for animals causing wound infections and mastitis [3,5].…”

Section: Introductionmentioning

confidence: 99%

“…S. sciuri has been shown to be an invasive pathogen for animals causing wound infections and mastitis [3,5]. Although principally animal species, S. sciuri may colonize humans and its isolation from clinical samples such as skin, vagina, blood, urine, central venous catheters has been reported [4,6–8]. Moreover, diseases in humans caused by this bacterium, such as wound infections, soft tissue infections, abscesses, boils, peritonitis and endocarditis, have been described [5,6,9–11].…”

Section: Introductionmentioning

confidence: 99%

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Possible virulence factors of Staphylococcus sciuri

Stepanović

Vuković

Trajkovič

et al. 2001

FEMS Microbiology Letters

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Staphylococcus sciuri is an opportunistic pathogen of controversial clinical significance. The factors that contribute to colonization and/or infection caused by this bacterium have not been studied intensively so far. The present research was carried out in order to study the presence of potential virulence factors in 121 human and animal isolates of this bacterium. Isolates were examined for biofilm formation, hemagglutination, presence of clumping factor, production of spreading factors and exotoxins, cytotoxicity and capacity to stimulate nitric oxide production. The results showed that S. sciuri is highly capable of biofilm production, that it displays strong proteolytic and DNase activities, produces hemolysins and stimulates nitric oxide production by rat macrophages. Although the present study showed existence of a wide spectrum of possible virulence determinants of S. sciuri, their exact contribution to virulence of this bacterium in vivo remains to be determined.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Possible virulence factors of Staphylococcus sciuri

Stepanović

Vuković

Trajkovič

et al. 2001

FEMS Microbiology Letters

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“…Kawano and colleagues (Kawano et al., 1996) described the isolation of S. sciuri isolates from healthy chickens being resistant to several classes of antibiotics. While isolates from humans, food‐producing animals or pets often proved to be resistant to one or more antimicrobial agents (Kloos et al., 1997; Slaughter et al., 2001; Stepanovic et al., 2001a), strains from wild animals (Kloos et al., 1997) also showed resistance patterns comparable with those described in strains from humans (Couto et al., 2000). In contrast, most of the strains obtained from rodents and insectivores showed an overall low level of antimicrobial resistance.…”

Section: Discussionmentioning

confidence: 98%

“…Marsou et al. (1999) also used ribotyping – although with a different probe as compared with Kloos et al. (1997)– and Dra I digestion of the PCR amplified spacer region between 16S and 23S rRNA.…”

Section: Discussionmentioning

confidence: 99%

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Differentiation of Staphylococcus sciuri Strains Isolated from Free‐Living Rodents and Insectivores

Hauschild

Schwarz

2003

Journal of Veterinary Medicine, Series B

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Twenty-nine Staphylococcus sciuri strains isolated from free-living insectivores and rodents were comparatively analysed for their biochemical capacities and their SmaI macrorestriction patterns. The 29 S. sciuri isolates represented 21 different biotypes and 22 different SmaI macrorestriction types. This observation confirmed that S. sciuri isolates obtained from insectivores and rodents living in natural environments constituted a heterogeneous population. Cluster analysis revealed that the macrorestriction patterns and the biochemical profiles matched in some cases. However, S. sciuri isolates that exhibited the same or closely related biochemical profiles were also found to be associated with different macrorestriction patterns. Analysis of the 29 S. sciuri isolates for their plasmid content and their sensitivity to antimicrobial agents showed that most of the isolates were plasmid-free and susceptible to all antimicrobial agents tested.

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Plasmid-specified FemABX-like immunity factor inStaphylococcus sciuriDD 4747

Heath

Gargis

Smithberg

et al. 2005

FEMS Microbiology Letters

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A plasmid from Staphylococcus sciuri DD 4747 had three open reading frames: a replication gene, an N-acetylmuramyl-l-alanine amidase-like gene, and a gene similar to the lysostaphin endopeptidase resistance gene (epr/lif). The epr-like gene was introduced into S. aureus RN4220; the recombinant strain was more resistant to lysostaphin endopeptidase and its cell wall peptidoglycan contained more serines and fewer glycines than the parental strain with the shuttle vector alone. Based on both its function and its similarity to femAB, this gene is a member of the femABX-like immunity gene family. Furthermore, this is the first example of a femABX-like immunity gene that is not linked to the gene for the bacteriolytic enzyme against which it specifies immunity.

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Ribotype Delineation and Description of Staphylococcus sciuri Subspecies and Their Potential as Reservoirs of Methicillin Resistance and Staphylolytic Enzyme Genes

Cited by 8 publications

References 0 publications

Possible virulence factors of Staphylococcus sciuri

Possible virulence factors of Staphylococcus sciuri

Differentiation of Staphylococcus sciuri Strains Isolated from Free‐Living Rodents and Insectivores

Plasmid-specified FemABX-like immunity factor inStaphylococcus sciuriDD 4747

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