Ribosomal RNA integrity and rate of seed germination

Brocklehurst, P. A.; Fraser, R. S. S.

doi:10.1007/bf02395308

Cited by 25 publications

(12 citation statements)

References 8 publications

(11 reference statements)

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“…These results suggest that the rRNAs undergo some degradation in the seed when in the dry state or during dessication. Similar results were reported for carrot and tobacco (Brocklehurst and Fraser 1980). However, as most of the preformed ribosomes are still able to function in protein synthesis, the structure of the rRNAs is probably not too affected by this degradation.…”

Section: Discussionsupporting

confidence: 88%

Polysome formation and incorporation of new ribosomes into polysomes during germination of the embryonic axis of maize

Dommès¹,

Walle²

1990

Physiol Plant

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Dommes, J. & Van de Walle, C. 1990. Polysome formation and incorporation of new ribosomes into poiysomes during germination of the embryonic axis of maize. P hysioi. Plant. 79: 289-296.Isolated axes of Zea mays L. cvs CiVj and CUZCO were imbibed for different periods of time, and free poiysomes were extracted and ,analysed by centrifugation in a sucrose gradient. The amount of rRNA per axis was determined at different moments of germination. Polysome reassembly was practically completed by 8 h and 54% of the preformed ribosomes were found in the polysome fraction. An increase in the proportion of large poiysomes was also observed during this period of germination. During the following period, tbe polysome content and the distribution of the various classes of poiysomes remained unchanged. The time of appearance of newly synthesized ribosomes into the polysomes was investigated using axes germinated in the presence of PH]-uridine. Centrifugal analysis of EDTA-dissociated poiysomes and gel electrophoretic analysis of polysomal RNA showed that new ribosomes appeared into poiysomes a few hours after completion of the initial polysome assembly. When released into the cytoplasm, the new ribosomes were preferentially incorporated into poiysomes rather than stored as free ribosomes.

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Section: Discussionsupporting

confidence: 88%

Polysome formation and incorporation of new ribosomes into polysomes during germination of the embryonic axis of maize

Dommès¹,

Walle²

1990

Physiol Plant

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“…d Semi-quantitative analysis by image analysis of 28S and 18S rRNAs (black and grey bars, respectively; means ± SE; n = 5 biological replicates of 20 ground seeds). Circles denote total germination as in b. Asterisks in b and d indicate differences between non-aged control seeds and Aged or dead seeds at a level of P \ 0.01 (one-way ANOVA) (Bray and Chow 1976;Boubriak et al 2000;Brockelhurst and Fraser 1980;Cheah and Osborne 1978;Osborne 2000). The final, or execution, phase of PCD is characterised by inter-nucelosomal DNA fragmentation (Elbaz et al 2002;Hengartner 2000;Thomas and Franklin-Tong 2004) and can include RNA degradation (Hoat et al 2006;Perrin et al 2004).…”

Section: Discussionmentioning

confidence: 97%

“…It is therefore surprising that the integrity of nucleic acids during seed ageing has received relatively little attention in the recent literature. Changes in nucleic acid content (Brockelhurst and Fraser 1980;Osborne 1974, 1977;Thompson et al 1987) and more recently, DNA fragmentation Osborne 2000), have been reported in seeds in relation to maturation and germination rate. Single and double strand breaks of DNA accumulate in ageing seeds (Tuteja et al 2001) and DNA fragmentation was correlated with seed death induced by drying in 'recalcitrant', i.e.…”

Section: Introductionmentioning

confidence: 99%

Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing

et al. 2010

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The germination of viable seeds is the basis for new plant growth and development. Seeds lose viability during storage, but the biochemical mechanisms of seed death are not fully understood. This study aimed to investigate degradation patterns of nucleic acids during seed ageing and subsequent water uptake. Seeds of Pisum sativum L. were artificially aged at 50°C and 12% seed water content (WC). Nucleic acids degradation was studied during ageing and during imbibition of four seed lots with differential viability from highly viable to dead. As seeds lost viability during ageing, DNA was gradually degraded into internucleosomal fragments, resulting in 'DNA laddering', in conjunction with disintegration of 18S and 28S rRNA bands. During imbibition, non-aged controls had high levels of DNA and RNA integrity through to radicle protrusion. In an aged seed lot with 85% total germination (TG) DNA fragmentation decreased upon imbibition probably due to nucleosome degradation, while rRNA integrity did not improve. In an aged seed lot with 44% TG, neither DNA nor rRNA integrity improved upon imbibition. Dead seeds showed DNA degradation as laddering throughout imbibition along with extensive degradation of rRNA. We present a model in which interlinked programmed and nonprogrammed events contribute to seed ageing, and suggest that protection of nucleic acids during ageing is key to seed longevity.

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“…The integrity of rRNA in aged seeds has been long studied [57][58][59][60][61] and RNA integrity number (RIN) was proposed to measure RNA quality [62,63]. Recently, Fleming and her colleagues applied RIN to assess seed aging status [29,30] and showed that RNA integrity decreased during seed aging in several plant species.…”

Section: The β Value Can Be Used To Describe the Rate Of Mrna Degradamentioning

confidence: 99%

Analysis of Stored mRNA Degradation in Acceleratedly Aged Seeds of Wheat and Canola in Comparison to Arabidopsis

Zhao

Wang

2020

Plants

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Seed aging has become a topic of renewed interest but its mechanism remains poorly understood. Our recent analysis of stored mRNA degradation in aged Arabidopsis seeds found that the stored mRNA degradation rates (estimated as the frequency of breakdown per nucleotide per day or β value) were constant over aging time under stable conditions. However, little is known about the generality of this finding to other plant species. We expanded the analysis to aged seeds of wheat (Triticum aestivum) and canola (Brassica napus). It was found that wheat and canola seeds required much longer periods than Arabidopsis seeds to lose seed germination ability completely under the same aging conditions. As what had been observed for Arabidopsis, stored mRNA degradation (∆Ct value in qPCR) in wheat and canola seeds correlated linearly and tightly with seed aging time or mRNA fragment size, while the quality of total RNA showed little change during seed aging. The generated β values reflecting the rate of stored mRNA degradation in wheat or canola seeds were similar for different stored mRNAs assayed and constant over seed aging time. The overall β values for aged seeds of wheat and canola showed non-significant differences from that of Arabidopsis when aged under the same conditions. These results are significant, allowing for better understanding of controlled seed aging for different species at the molecular level and for exploring the potential of stored mRNAs as seed aging biomarkers.

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Ribosomal RNA integrity and rate of seed germination

Cited by 25 publications

References 8 publications

Polysome formation and incorporation of new ribosomes into polysomes during germination of the embryonic axis of maize

Polysome formation and incorporation of new ribosomes into polysomes during germination of the embryonic axis of maize

Inter-nucleosomal DNA fragmentation and loss of RNA integrity during seed ageing

Analysis of Stored mRNA Degradation in Acceleratedly Aged Seeds of Wheat and Canola in Comparison to Arabidopsis

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