Ribosomal RNA Genes of Phaseolus coccineus. IV. Structure and Comparative-analysis of the Intergenic Spacer: Possible Involvement of Some Nucleotides in the Transcription Control of Coding Sequences Sequence of the Intergenic Spacer of Ribosomal Genes

Marrocco, R.; Castiglione, Monica Ruffini; Ruberti, Francesca; Maggini, F.; Avanzi, S.

doi:10.3109/10425179809050024

Cited by 2 publications

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“…Regulatory elements in the IGS, such as promoters, enhancers and terminators, play an essential part in the control of rRNA transcription (Reeder 1984;Flavell 1993), in processing of the transcript and probably in rep-lication of the unit (Reeder 1990;Alvares et al 1998;Marrocco et al 1998). Variation in the length and structure of the IGS often results from variation in the number of subrepeat units and, consequently, in copy number of the regulatory elements (Reeder 1990;Moss & Stefanovsky 1995).…”

Section: Introductionmentioning

confidence: 99%

Functional and ecological significance of rDNA intergenic spacer variation in a clonal organism under divergent selection for production rate

Gorokhova

Dowling

Weider

et al. 2002

Proc. R. Soc. Lond. B

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It has recently been hypothesized that variation in the intergenic spacer (IGS) of rDNA has considerable developmental, evolutionary and ecological significance through effects on growth rate and body C : N : P stoichiometry resulting from the role of the IGS in production of rRNA. To test these ideas, we assessed changes in size and structure of the repetitive region of the IGS, juvenile growth rate (JGR), RNA and phosphorus (P) contents in clonal lineages of Daphnia pulex derived from a single female and subjected to divergent selection on weight-specific fecundity (WSF). As a result of selection, WSF diverged rapidly, with significant reductions within two generations. Other significant changes accompanying shifts in WSF were that juveniles produced by low-WSF females grew more rapidly and had higher RNA and P contents. An increased predominance of long IGS variants was observed in lineages with elevated JGRs and low WSF. The observed variations in IGS length were related to the number of subrepeat units carrying a promoter sequence in the repetitive region. These results strongly support the hypothesized relationships, indicate a genetic mechanism for the evolution of such associations and demonstrate that Daphnia (and perhaps other parthenogens) possess considerable potential for rapid adaptive change in major lifehistory traits.

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Section: Introductionmentioning

confidence: 99%

Functional and ecological significance of rDNA intergenic spacer variation in a clonal organism under divergent selection for production rate

Gorokhova

Dowling

Weider

et al. 2002

Proc. R. Soc. Lond. B

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Ribosomal RNA genes of Phaseolus coccineus V. Relationship between rDNA phenotype and somatic differentiation

et al. 1998

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Summary. Size variations in the intergenic spacer of ribosomalDNA were detected between individual plants of openly pollinated Phaseolus coccineus. Eleven days after sowing, two plant samples were examined: slowly developing plants with a length less than 40 cm; and fast developing plants with a length greater than 70 cm. The two samples were characterized by different plant weight and, at maturity, by highly distinctive seed yield. They also exhibited distinct patterns of protein expression as analyzed by 2-D electrophoresis. In particular a 38 kDa protein, related to malate dehydrogenase on the basis of its N-terminal sequence, was present at higher concentration and higher activity levels in fast developing plants. Intergenic spacer length variants were detected in both samples at approximately 180 bp intervals. More than one spacer length variant was present in each individual plant. At least 13 different intergenic spacer hybridization patterns were in fact detected: some patterns occurred equally in both slowly and fast developing samples while the majority of patterns was significantly different between the two samples.Keywords: Two-dimensional

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Ribosomal RNA Genes of Phaseolus coccineus. IV. Structure and Comparative-analysis of the Intergenic Spacer: Possible Involvement of Some Nucleotides in the Transcription Control of Coding Sequences Sequence of the Intergenic Spacer of Ribosomal Genes

Cited by 2 publications

References 10 publications

Functional and ecological significance of rDNA intergenic spacer variation in a clonal organism under divergent selection for production rate

Functional and ecological significance of rDNA intergenic spacer variation in a clonal organism under divergent selection for production rate

Ribosomal RNA genes of Phaseolus coccineus V. Relationship between rDNA phenotype and somatic differentiation

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