Ribosomal Ribonucleic Acid Cistron Similarities and Deoxyribonucleic Acid Homologies of Neisseria, Kingella, Eikenella, Simonsiella, Alysiella, and Centers for Disease Control Groups EF-4 and M-5 in the Emended Family Neisseriaceae

Abstract

Federal Republic of Germany2 We detected distinct taxonomic relationships among the true Neisseria species, Kingella kingae, Kingella denitrGcans, Eikenella corrodens, all Simonsiella species, the type strain of Alysiellafiliformis, and members of Centers for Disease Control groups EF-4 and M-5. All these taxa constitute one large separate cluster having high levels of ribosomal ribonucleic acid cistron similarity (thermal denaturation temperature range, 74 to 81°C) in ribosomal ribonucleic acid superfamily 111. There are at least four subbranches. We found high deoxyribonucleic acid (DNA)-DNA homology values between Neisseria gonorrhoeae and some other true Neisseriu species and within the following species: Simonsiella muelleri, Simonsiella crassa, Simonsiella steedue, Kingella denitrificans, and Eikenella corrodens. All of the members of this large cluster have genome base compositions in the range from 42.8 to 57.7 mol% guanine plus cytosine. The molecular complexities of the genomic DNAs are 2.2 X lo9 to 2.7 x lo9 for Sirnonsiella and Alysiella species and 1.4 X lo9 to 1.8 X lo9 for * Corresponding author.

Uruburuella suis gen. nov., sp. nov., isolated from clinical specimens of pigs

“…Phylogenetically, the family encompasses the genera Neisseria, Kingella, Eikenella, Simonsiella and Alysiella (Dewhirst et al, 1989;Rossau et al, 1989). It is clear from the results of the present study that the unidentified bacterium from pigs is phylogenetically a member of this family grouping.…”

“…On the basis of rRNA cistron similarity studies, Rossau et al (1986) recommended that the genera Acinetobacter, Moraxella and Branhamella be excluded from the family Neisseriaceae. Subsequent studies by Rossau et al (1989) showed that the genera Neisseria, Kingella, Eikenella, Simonsiella and Alysiella were closely related and should be classified together in the family Neisseriaceae, which comprises a major branch of the b-Proteobacteria. Comparative 16S rRNA gene sequencing studies by Dewhirst et al (1989) confirmed the results of Rossau et al (1989) and led to the recommendation that the genera Eikenella and Simonsiella be transferred to the family Neisseriaceae.…”

“…The family Neisseriaceae has undergone various revisions over the years, and numerous other genera have been assigned to it [e.g. Psychrobacter (Juni & Heym, 1986), Mesophilobacter (Nishimura et al, 1989), Alysiella (Rossau et al, 1989) and Eikenella and Simonsiella (Dewhirst et al, 1989)]. On the basis of rRNA cistron similarity studies, Rossau et al (1986) recommended that the genera Acinetobacter, Moraxella and Branhamella be excluded from the family Neisseriaceae.…”

Transfer of Kingella indologenes (Snell and Lapage 1976) to the Genus Suttonella gen. nov. as Suttonella indologenes comb. nov.; Transfer of Bacteroides nodosus (Beveridge 1941) to the Genus Dichelobacter gen. nov. as Dichelobacter nodosus comb. nov.; and Assignment of the Genera Cardiobacterium, Dichelobacter, and Suttonella to Cardiobacteriaceae fam. nov. in the Gamma Division of Proteobacteria on the Basis of 16S rRNA Sequence Comparisons

“…This problem is being encountered with increasing frequency as ribosomal cistron analysis and 16s rRNA sequencing studies show that organisms with major phenotypic differences are closely related genomically. For example, the facultatively anaerobic organism Eikenella corrodens is clearly a member of the aerobic family Neisseriaceae (10,28). In addressing this problem, we were guided by the advice presented by Wayne et al (44).…”

“…Kingella indologenes has been recovered from human eye infections and from the blood of a patient with endocarditis (15,39,42). It has been reported previously that Kingella indologenes is not closely related to other Kingella species (10,28) and thus is generically misnamed. Bacteroides nodosus is the essential causative agent of footrot in sheep, * Corresponding author.…”