Transfer of Kingella indologenes (Snell and Lapage 1976) to the Genus Suttonella gen. nov. as Suttonella indologenes comb. nov.; Transfer of Bacteroides nodosus (Beveridge 1941) to the Genus Dichelobacter gen. nov. as Dichelobacter nodosus comb. nov.; and Assignment of the Genera Cardiobacterium, Dichelobacter, and Suttonella to Cardiobacteriaceae fam. nov. in the Gamma Division of Proteobacteria on the Basis of 16S rRNA Sequence Comparisons

Dewhirst, Floyd E.; Paster, Bruce J.; Fontaine, Sharon La; Rood, Julian I.

doi:10.1099/00207713-40-4-426

Cited by 133 publications

(37 citation statements)

References 32 publications

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“…The organization of the D. nodosus fur region was different from that of other characterized fur regions, although some conservation exists among members of the ␥ subclass of the Proteobacteria (37), to which D. nodosus belongs (13). The presence of an rpoH gene upstream of the fur gene was unique to D. nodosus, and although rpoH was not part of an operon with fur and there was no sigma32 consensus binding site in the fur promoter region, we cannot rule out the possibility that Fur may regulate rpoH, causing a discrete set of genes to be expressed, as observed in S. oneidensis (58).…”

Section: Discussionmentioning

confidence: 99%

Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets

Parker

Kennan

Myers³

et al. 2005

J Bacteriol

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The expression of iron regulated genes in bacteria is typically controlled by the ferric uptake regulator (Fur) protein, a global transcriptional repressor that regulates functions as diverse as iron acquisition, oxidative stress, and virulence. We have identified a fur homologue in Dichelobacter nodosus, the causative agent of ovine footrot, and shown that it complements an Escherichia coli fur mutant. Homology modeling of the D. nodosus Fur protein with the recently solved crystal structure of Fur from Pseudomonas aeruginosa indicated extensive structural conservation. As Southern hybridization analysis of different clinical isolates of D. nodosus indicated that the fur gene was present in all of these strains, the fur gene was insertionally inactivated to determine its functional role. Analysis of these mutants by various techniques did not indicate any significant differences in the expression of known virulence genes or in iron-dependent growth. However, we determined several Fur regulatory targets by two-dimensional gel electrophoresis coupled with mass spectrometry. Analysis of proteins from cytoplasmic, membrane, and extracellular fractions revealed numerous differentially expressed proteins. The transcriptional basis of these differences was analyzed by using quantitative reverse transcriptase PCR. Proteins with increased expression in the fur mutant were homologues of the periplasmic iron binding protein YfeA and a cobalt chelatase, CbiK. Down-regulated proteins included a putative manganese superoxide dismutase and ornithine decarboxylase. Based on these data, it is suggested that in D. nodosus the Fur protein functions as a regulator of iron and oxidative metabolism.

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Section: Discussionmentioning

confidence: 99%

Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets

Parker

Kennan

Myers³

et al. 2005

J Bacteriol

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“…This low similarity level again indicates that there is a large genetic distance between the new taxon and its closest relatives. Also from the viewpoint of direct sequencing, this genetic distance corresponds to the genomic range within several bacterial families (e.g., the family Cardiobacteriaceae, whose members cluster at a similarity level of more than 93% [15]). We therefore propose a new genus, Omithobacteri'urn, and a new species, 0. rhinotracheale, to accommodate this taxon.…”

Section: Discussionmentioning

confidence: 99%

Ornithobacterium rhinotracheale gen. nov., sp. nov., Isolated from the Avian Respiratory Tract

Vandamme

Segers

Vancanneyt

et al. 1994

International Journal of Systematic Bacteriology

151

104

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The phylogenetic position and various genotypic, chemotaxonomic, and classical phenotypic characteristics of 21 gram-negative avian isolates were studied. These strains constitute a genotypically homogeneous taxon in rRNA superfamily V, as shown by DNA-rRNA hybridization data. Determination of the 16s rRNA sequence of this taxon revealed its detailed position within the "flavobacter" subgroup of the "flavobacter-bacteroides" phylum as described by Gherna and Woese (R. Gherna and C. R. Woese, Syst. Apple Microbiol. 15513-521, 1992). This new taxon is only distantly related to other members of the "flavobacter-bacteroides" phylum and is therefore given separate generic status. The DNA-DNA binding values for members of this taxon, for which we propose the name Ornithobacterium rhinotracheale, confirmed that all of the strains are highly interrelated (DNA-DNA binding values greater than 90% were measured). The G+C contents of members of this taxon are between 37 and 39 mol%, An analysis of the cellular proteins and fatty acids and classical phenotypic characteristics allowed us to distinguish 0. rhinotracheafe from phenotypically similar taxa, such as Riemrelliz anatipestiyer and Capnocytophaga species. The respiratory quinone content (menaquinone 7) and carbohydrate pattern of 0 . rhinotracheale conform with the respiratory quinone contents and carbohydrate patterns of other members of rRNA superfamily V.In the past decade, we determined the protein and fatty acid profiles of a large number of avian isolates which could not be classified after primary identification tests were performed in veterinary laboratories. A total of 21 of these isolates had very similar profiles and differed clearly from all of the other strains investigated, including reference strains of well-known fowl pathogens. These 21 strains were isolated from the respiratory tracts of turkeys (10 strains), chickens (7 strains), rooks, (3 strains), and a partridge (1 strain). Clinical data were not available for all of these strains. However, most of the strains were associated with various respiratory tract infections, including tracheitis, pericarditis, sinusitis, airsacculitis, and pneumonia. Preliminary data on some of the chemotaxonomic characteristics of four strains have been described previously (36).We studied a wide range of taxonomic parameters, including genotypic, chemotaxonomic, and classical phenotypic parameters, in order to establish the phylogenetic affiliation and to comprehensively describe the new taxon, for which we propose the name Omithobacterium rhinotracheale. Special emphasis was given to the differentiation of 0. rhinotracheale, Riemerella anatipestifer, and Capnocytophaga species as these taxa share a number of phenotypic characteristics. * Corresponding author. Mailing address: Laboratorium voorMicrobiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium. Phone: 32-9-2645114. Fax: 32-9-2645346. MATERIALS AND METHODSIsolation of 0. rhinotracheale strains. Samples were inoculated onto media routinel...

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“…This Gram-negative anaerobic rod-shaped bacterium belongs to the Gammaproteobacteria [Dewhirst et al, 1990;La Fontaine et al, 1990] and represents the essential causative agent of ovine footrot. Footrot is regarded as the most important bacterial disease of sheep, resulting in heavy economic losses in wool and meat industries.…”

Section: Introductionmentioning

confidence: 99%

Analyses of the vrl Gene Cluster in Desulfococcus multivorans: Homologous to the Virulence-Associated Locus of the Ovine Footrot Pathogen Dichelobacter nodosus Strain A198

et al. 2007

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Major parts of the virulence-associated vrl locus known from the gammaproteobacterium Dichelobacter nodosus, the causative agent of ovine footrot, were analyzed in the genome of the sulfate-reducing deltaproteobacterium Desulfococcus multivorans. In the genome of D. multivorans 13 of the 19 vrl genes described for D. nodosus are present and highly conserved with respect to gene sequence and order. The vrl locus and its flanking regions suggest a bacteriophage-mediated transfer into the genome of D. multivorans. Comparative analysis of the deduced Vrl proteins reveals a wide distribution of parts of the virulence-associated vrl locus in distantly related bacteria. Horizontal transfer is suggested as driving mechanism for the circulation of the vrl genes in bacteria. Except for the vrlBMN genes D. multivorans and Desulfovibrio desulfuricans G20 together contain all vrl genes displaying a high degree of similarity. For D. multivorans it could be shown that guanine plus cytosine (GC) content, GC skew, di-, tri- or tetranucleotide distribution did not differ between the vrl locus and its flanking sequences. This could be a hint that the vrl locus originated from a related organism or at least a genome with similar characteristics. The conspicuous high degree of conservation of the analyzed vrl genes may result from a recent transfer event or reflect a function of the vrl genes, which is still unknown and not necessarily disease associated. The latter is supported by the evidence for expression of the vrl genes in D. multivorans, which has not been described as pathogen or to be associated to any disease pattern before.

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Cited by 133 publications

References 32 publications

Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets

Identification of a Dichelobacter nodosus Ferric Uptake Regulator and Determination of Its Regulatory Targets

Ornithobacterium rhinotracheale gen. nov., sp. nov., Isolated from the Avian Respiratory Tract

Analyses of the <i>vrl</i> Gene Cluster in <i>Desulfococcus multivorans</i>: Homologous to the Virulence-Associated Locus of the Ovine Footrot Pathogen <i>Dichelobacter nodosus</i> Strain A198

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