Ribonucleic Acid-Induced Changes in Mammalian Cells

Induction of Myeloma Protein Synthesis in Ehrlich Ascites Tumor Cells by the Addition of a Plasma Cell Tumor (X5563) Rna

“…Concerning protein synthesis in mammalian cells induced by the extracellular RNA, Niu et al (1961Niu et al ( , 1962 …”

“…For instance, in a cell free system prepared from rabbit reticulocytes, a messenger RNA-like fraction obtained from sheep reticulocytes could produce sheep hemoglobin (Lamfrom 1962). In intact mammalian cells, other heterologous proteins could be synthesized by the addition of exogenous RNA (Niu et al 1961; Weisberger 1962) or DNA (Szybalska and Szybalski 1963).The present study represents an attempt to obtain molecular genetic information (RNA) related to myeloma protein synthesis. Specifically, the attempt was made to induce synthesis of gamma myeloma protein by Ehrlich ascites tumor cells by exposing these cells to a high concentration of RNA extracted from a plasma cell tumor.…”

“…For instance, in a cell free system prepared from rabbit reticulocytes, a messenger RNA-like fraction obtained from sheep reticulocytes could produce sheep hemoglobin (Lamfrom 1962). In intact mammalian cells, other heterologous proteins could be synthesized by the addition of exogenous RNA (Niu et al 1961;Weisberger 1962) or DNA (Szybalska and Szybalski 1963).…”

Formation of Tryptophan-pyrrolase in Leukaemic Cells Incubated with RNA Obtained from the Spleen of Calf Embryo

“…Incubated with RNA Obtained from the Spleen of Calf Embryo By Sergio E sposito Over the last few years various writers have demonstrated the possibility of affecting the evolution of certain tissues by incubating them in a culture containing RNA (1,3,10). It has been observed, for example, that when an embryonic tissue is cultivated with the addition of RNA extracted from different organs, its own develop ment is orientated towards forms similar to those of the tissue which originally furnished the RNA (6,9,12).…”

Poly(A)-Attached RNA as Activator in Embryonic Differentiation

“…These RNA-treated cells had increased synthesis not only of RNA (9) but also of specific protein (3)(4)(5)(6)(7). The RNA-stimulated activity was sensitive to actinomycin D (9).…”