Ribonucleic Acid-Dependent Deoxyribonucleic Acid Polymerase in Visna Virus

Lin, Fu Hai; Thormar, Halldór

doi:10.1128/jvi.6.5.702-704.1970

Cited by 88 publications

(23 citation statements)

References 7 publications

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“…It contains a poly(A) segment of large and homogeneous size (9). The virus particle, whose structure is very close to that of RNA tumor viruses (18,26), contains an RNA-dependent DNA polymerase (15,20,23) and replicates through the intermediate of a DNA provirus (11). Lastly, Visna virus has been reported to transform murine cells in vitro (25).…”

mentioning

confidence: 99%

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Properties of Visna Virus Particles Harvested at Short Time Intervals: RNA Content, Infectivity, and Ultrastructure

Brahic

Vigne

1975

J Virol

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and ethylenediacrylate were from Eastman Organic Chemicals.Acrylami'de and bisacrylamide were from Biorad.RNase-free sucrose, used for RNA work, was purchased from Schwarz-Mann.[3H ]uridine (20 Ci/mM) and carrier-free sP were from CEA, France. All other chemicals were obtained as reagent grade.TNE buffer refers to the following solution: 0.01 M Tris-hydrochloride (pH 7.5), 0.1 M NaCl, and 0.001 M EDTA. TNE-SDS buffer refers to the same solution containing 0.5% sodium dodecyl sulfate (SDS). For the RNA work, the buffers and plastic pieces of equipment were treated with diethylpyrocarbonate 1222 on July 7, 2020 by guest

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confidence: 99%

“…VOL. 15,1975 After purification, an aliquot of the virus suspension was centrifuged in a 10 to 50% linear potassium tartrate gradient (data not shown). The virus banded at a density of 1.17 g/ml, a density identical to that of older virus.…”

mentioning

confidence: 99%

Properties of Visna Virus Particles Harvested at Short Time Intervals: RNA Content, Infectivity, and Ultrastructure

Brahic

Vigne

1975

J Virol

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“…RNA viruses containing an RNA-directed DNA polymerase (RDDP) have been associated with a variety of chronic infections in animals, including neoplasms (26) and encephalitis (27,28). Of particular interest are the reports that oncogenic RNA viruses can be implicated in the immune complex glomerulonephritis of New Zealand black mice (29) and possibly in systemic lupus erythematosus (2,30).…”

Section: Discussionmentioning

confidence: 99%

DNA polymerase activity of cultured rheumatoid synovial cells

Spruance

Richards

Smith

et al. 1975

Arthritis & Rheumatism

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Cultured synovial cells from 8 patients with rheumatoid arthritis (RA) and 7 subjects without joint disease were assayed and compared for RNA‐directed and DNA‐directed DNA polymerase activity. No activity was found in either RA or control specimens using a synthetic RNA template that specifically detects oncornavirus RNA‐directed DNA polymerase. The DNA‐directed DNA polymerase activity of RA specimens was increased (P <0.10) in the high‐speed pellet fraction of cell lysates. The possible relationship of these finding to virus infection in RA is discussed.

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“…Many lytic and tumorigenic viruses contain nucleic acid-polymerizing enzymes in their virions (3,4,11,16,24,26,28). Little information is available concerning regulatory mechanisms of virion-associated enzyme activities during virus replication and tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

DNA-Dependent RNA Polymerase Activity Associated with Subviral Particles of Polyhedral Cytoplasmic Dexoyribovirus

Gaby

Kucera

1974

J Virol

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Polyhedral cytoplasmic deoxyribovirus virions contain a DNA-dependent RNA polymerase which catalyzes the incorporation of ribonucleotides into an acid-precipitable product. Treatment of virions with sodium deoxycholate and dithiothreitol resulted in the formation of subviral particles which could be separated from virions by rate zonal centrifugation in sucrose gradients. Subviral particles were RNA polymerase-positive and more active per unit mass of protein than virions. In vitro enzyme activity associated with subviral particles required addition of ribonucleotides, Mg2+, and exogenous denatured DNA template. Optimal enzyme activity occurred over a broad pH (7.2 to 8.8) and Mg2+ concentration (2 to 10 limol) range. The specific activity of the RNA polymerase was maximal at 37 C. Addition of DNase or actinomycin D to the reaction mixture reduced the incorporation of [3H ]UMP into an acid-precipitable product. The product of the reaction was sensitive to degradation by RNase but not to DNase or Pronase. These data suggest that the enzyme copies DNA into RNA. serum albumin, dithiothreitol, ATP, GTP, CTP, UTP, and DNase I (pancreatic, RNase free, electrophoretically purified) from Sigma Chemical Co.;RNase (pancreatic, 2x crystallized) from Worthington Biochemicals; actinomycin D, rifampin, aamanitin, and pronase from Calbiochem; sodium deoxycholate from Fisher Scientific; poly(dA-dT) from P-L Biochemicals, Inc.; Liquifluor, [3H JTdR (20 Ci/mmol), and ["CC]TdR (50 mCi/mmol) from New England Nuclear; and [3H]UTP (12.6 Ci/mmol) and sucrose (RNase free) from Schwarz/Mann. Preparation of purified PCDV virions. A 20x cell-associated virus (CAV) stock was prepared from PCDV-infected fathead minnow cells as previously described (14). The resulting CAV stock was treated with DNase (100 Ag/ml of CAV suspension, 37 C, 30 min) and centrifuged (SW27 rotor, 52,000 x g, 2 h, 10 C) through 10% (wt/wt) potassium tartrate in 0.01 M Tris buffer (TB) (pH 7.8) (8). The virus pellets were resuspended in 0.05 M sodium phosphate-buffered saline, pH 7.2, supplemented with 0.5% bovine serum albumin to 1/20 the original volume of the 20x CAV 231 on July 6, 2020 by guest

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Ribonucleic Acid-Dependent Deoxyribonucleic Acid Polymerase in Visna Virus

Cited by 88 publications

References 7 publications

Properties of Visna Virus Particles Harvested at Short Time Intervals: RNA Content, Infectivity, and Ultrastructure

Properties of Visna Virus Particles Harvested at Short Time Intervals: RNA Content, Infectivity, and Ultrastructure

DNA polymerase activity of cultured rheumatoid synovial cells

DNA-Dependent RNA Polymerase Activity Associated with Subviral Particles of Polyhedral Cytoplasmic Dexoyribovirus

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