1998
DOI: 10.1590/s0001-37141998000300008
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Ribonuclease Production by Aspergillus species

Abstract: Ribonuclease production by Aspergillus flavipes, A. sulphureus and A. fischeri in semi-synthetic medium, after 24-144 hours at 30ºC under shaking, was studied. After cultivation, the medium was separated from micelia by filtration and the resultant solution was used as enzymatic extract. The highest amount of biomass and RNase was obtained after 96 hours of cultivation. The enzymes produced by three species presented similar characteristics, with optimum temperature at 55ºC and two peaks of activity at pH 4.5 … Show more

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Cited by 9 publications
(3 citation statements)
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“…Till date, few papers have reported optimization of production conditions for extracellular microbial RNases 3,8,33,37 . Optimized conditions enhance the production quantitatively as well as qualitatively.…”
Section: Introductionmentioning
confidence: 99%
“…Till date, few papers have reported optimization of production conditions for extracellular microbial RNases 3,8,33,37 . Optimized conditions enhance the production quantitatively as well as qualitatively.…”
Section: Introductionmentioning
confidence: 99%
“…Dentre eles, os fungos filamentosos ganham destaque por serem mais adaptados ao crescimento em substratos sólidos devido à formação de hifas, o que favorece a sua rápida colonização (DURAND, 2003). Esses microrganismos são ainda de grande interesse devido à sua capacidade de secretar enzimas extracelulares que degradam a biomassa (GOMES et al, 1998;BANSAL et al, 2012;BAFFI, 2014). Um exemplo são as espécies do gênero…”
Section: Microrganismos Produtores De Enzimasunclassified
“…Entre as linhagens testadas, o fungo Aspergillus niger AHU 7117 apresentou grande potencial de síntese da enzima e especificidade para essa aplicação, apresentando valores acima de 300UmL -1 . Gomes et. al (1998) avaliaram a produção de ribonucleases pelas linhagens de Aspergillus flavipes, A. sulphureus e A. fischeri em meio de cultura semi sintético composto por 5% de glicose, 0,5% de extrato de carne, 0,2% de extrato de levedura, 0,05% de sulfato de magnésio, 0,01% de cloreto de cálcio, 0,02% de nitrato de potássio, e 0,5% de farelo de soja.…”
Section: Fontes Microbianas De Nuclease P1 Ou 5´fosfodiesteraseunclassified