“…Biological methods are also being used (El-Sadr, Macrae and Work, 1940;Copping, 1943). Of the microbiological test d l that need be said at the moment 88 PROCEEDINGS OF THE NUTRITION SOCIETY is that further critical examination seems to be needed of the effects of the various interfering factors, which may act as inhibitors (Kodicek andWorden, 1944, 1915) or as accelerators (Bauernfeind, Sotier and in the synthesis of fat from protein (McHenry and Gavin, 1941) and in the metabolism of tryptophane (Reid, Lepkovsky, Bonner and Tatum, 1944).…”