Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis

Qin, Qiaoji; Cui, Liqiang; Li, Peng; Wang, Yong‐Bin; Zhang, Xue‐Zhi; Guo, Ming‐Lei

doi:10.3892/mmr.2019.9908

Cited by 15 publications

(15 citation statements)

References 43 publications

(39 reference statements)

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“…Uncaria rhynchophylla , a traditional Chinese medicine, is usually used in traditional Chinese medicine formulas for the treatment of asthma. Rhynchophylline (Rhy), a kind of indole alkaloid isolated from Uncaria rhynchophylla , shows anti-inflammation and anti-oxidant capabilities [ 6 , 7 ]. Our previous study showed that rhynchophylline can mitigate inflammation in asthma, attenuate hypertrophy of bronchial smooth muscle cells [ 8 ], however, the effect of rhynchophylline on autophagy in asthma remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma

Li¹,

Cui³

et al. 2021

BMC Complement Med Ther

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Background Asthma is a chronic inflammatory disease characterized by airway remodeling and inflammation. Rhynchophylline is a kind of indole alkaloid isolated from Uncaria rhynchophylla. Here we investigated the effect of rhynchophylline on autophagy in asthma. Methods A mice model of asthma was established by ovalbumin challenge. Histopathological changes were assessed by hematoxylin-eosin staining, periodic acid-schiff staining and Masson staining. The levels of IgE in serum, interleukin-6 and interleukin-13 in bronchoalveolar lavage fluid, as well as the activities of superoxide dismutase and catalase in lung tissues were detected. The expression of autophagy-related genes and Janus kinase (JAK) 2/ signal transducer and activator of transcription (STAT) 3 signal was detected by western blot and immunofluorescence. Airway smooth muscle cells (ASMCs) were isolated, and the effect rhynchophylline on autophagy in ASMCs was explored. Results Our data showed that rhynchophylline treatment alleviated inflammation, airway remodeling, and oxidative stress in asthma. In addition, autophagy, which was implicated in asthma, was suppressed by rhynchophylline with decreased level of autophagy-related proteins. Furthermore, rhynchophylline suppressed the JAK2/STAT3 signaling pathway, which was activated in asthma. In vitro study showed that rhynchophylline suppressed ASMC autophagy through suppressing the activation of JAK2/STAT3 signal. Conclusions Our study demonstrated that rhynchophylline can alleviate asthma through suppressing autophagy in asthma, and that JAK2/STAT3 signal was involved in this effect of rhynchophylline. This study indicates that rhynchophylline may become a promising drug for the treatment of asthma.

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Section: Introductionmentioning

confidence: 99%

Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma

Li¹,

Cui³

et al. 2021

BMC Complement Med Ther

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“…Alkaloid compounds have the effect of protecting the cardiovascular system. For example, rhynchophylline can improve myocardial ischemia‐reperfusion injury of myocardial cells [43].…”

Section: Resultsmentioning

confidence: 99%

“…Sixty flavonoids were identified in this study, corresponding to compounds 12,16,19,26,34,38,39,43,44,49,50,52,55,60,61,62,63,69,70,75,77,78,80,81,83,86,87,89,90,91,92,93,94,96,99,100,101,102,103,104,105,107,108,110,111,113,117,118,119,120,121,122,123,124,125,126,128,129,130, and 131 in Figure 4, and the mass spectrum information in…”

Section: Characterization Of Flavonoidsmentioning

confidence: 99%

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Wang

et al. 2020

J of Separation Science

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Duzhong Jiangya tablet is a hypotensive drug. In this study, high-performance liquid chromatography-Fourier transform-ion cyclotron resonance-mass spectrometry technology was used to quickly identify its chemical composition. SinoChrom ODS-BP column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile(A)-0.1% formic acid solution(B). The flow rate was 1 mL/min. Extracted ion chromatogram was used to analyze the samples in positive and negative ion modes. Based on the accurate mass spectrometry information (such as quasi-molecular ions and fragment ions) obtained from the instrument, combined with reference compounds and literature, the chemical composition of Duzhong Jiangya Tablets was identified. A total of 131 compounds were identified, including four types of penylpropanoids, six types of phenylethanoid glycosides, 10 types of organic acids, 14 types of iridoids, 12 types of lignans, 18 types of alkaloids, seven types of coumarins, and 60 kinds of flavonoids. This established method can quickly and efficiently identify chemical constituents in Duzhong Jiangya tablet, lay a foundation for the research on the efficacy and quality of this traditional Chinese medicine, and provide a reference for the characterization of the chemical constituents of other traditional Chinese medicine preparations. K E Y W O R D S chemical composition, Duzhong Jiangya Tablet, Fourier transform ion cyclotron resonance, traditional Chinese medicine 1 INTRODUCTION Varieties of medicinal materials in traditional Chinese medicine compound preparations have complex chemical components. The synergy between the chemical components is the material basis for the traditional Chinese medicine preparations to exert their pharmaco-Article Related Abbreviations: ICR, ion cyclotron resonance logical effects [1]. The active ingredients of most Chinese medicines are still unknown, although there are many reports on Chinese medicine research. Therefore, it is very important to establish a fast and efficient method to identify the chemical components in traditional Chinese medicine. This can reasonably explain the material basis of the efficacy of traditional Chinese medicine. Duzhong Jiangya Tablet consists of Eucommia ulmoides Oliv, Leonurus japonicus Houtt, Prunella vulgaris L,

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“…In similar experimental conditions, U. Rhyncophylla has been shown to favor anti-apoptotic over pro-apoptotic proteins in vitro [ 207 ]. Moreover, Rhy, Isorhy and U. Rhyncophylla were all shown to prevent the increase of caspase-3 cleavage in various models of neurotoxicity [ 70 , 79 , 94 , 98 , 207 , 208 ]. The cleavage of caspase-3, known as an ‘executor of apoptosis’, is often considered the ultimate step in the apoptotic cascade [ 209 ].…”

Section: Rhy Targets and Links To Sleep Regulationmentioning

confidence: 99%

Cellular Effects of Rhynchophylline and Relevance to Sleep Regulation

et al. 2021

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Uncaria rhynchophylla is a plant highly used in the traditional Chinese and Japanese medicines. It has numerous health benefits, which are often attributed to its alkaloid components. Recent studies in humans show that drugs containing Uncaria ameliorate sleep quality and increase sleep time, both in physiological and pathological conditions. Rhynchophylline (Rhy) is one of the principal alkaloids in Uncaria species. Although treatment with Rhy alone has not been tested in humans, observations in rodents show that Rhy increases sleep time. However, the mechanisms by which Rhy could modulate sleep have not been comprehensively described. In this review, we are highlighting cellular pathways that are shown to be targeted by Rhy and which are also known for their implications in the regulation of wakefulness and sleep. We conclude that Rhy can impact sleep through mechanisms involving ion channels, N-methyl-d-aspartate (NMDA) receptors, tyrosine kinase receptors, extracellular signal-regulated kinases (ERK)/mitogen-activated protein kinases (MAPK), phosphoinositide 3-kinase (PI3K)/RAC serine/threonine-protein kinase (AKT), and nuclear factor-kappa B (NF-κB) pathways. In modulating multiple cellular responses, Rhy impacts neuronal communication in a way that could have substantial effects on sleep phenotypes. Thus, understanding the mechanisms of action of Rhy will have implications for sleep pharmacology.

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Rhynchophylline ameliorates myocardial ischemia/reperfusion injury through the modulation of mitochondrial mechanisms to mediate myocardial apoptosis

Cited by 15 publications

References 43 publications

Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma

Suppression of autophagy through JAK2/STAT3 contributes to the therapeutic action of rhynchophylline on asthma

Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Cellular Effects of Rhynchophylline and Relevance to Sleep Regulation

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