Rapid characterization of the chemical constituents of Duzhong Jiangya tablet by HPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry

Abstract

Duzhong Jiangya tablet is a hypotensive drug. In this study, high-performance liquid chromatography-Fourier transform-ion cyclotron resonance-mass spectrometry technology was used to quickly identify its chemical composition. SinoChrom ODS-BP column (250 mm × 4.6 mm, 5 μm) was used. The mobile phase was acetonitrile(A)-0.1% formic acid solution(B). The flow rate was 1 mL/min. Extracted ion chromatogram was used to analyze the samples in positive and negative ion modes. Based on the accurate mass spectrometry information (such as quasi-molecular ions and fragment ions) obtained from the instrument, combined with reference compounds and literature, the chemical composition of Duzhong Jiangya Tablets was identified. A total of 131 compounds were identified, including four types of penylpropanoids, six types of phenylethanoid glycosides, 10 types of organic acids, 14 types of iridoids, 12 types of lignans, 18 types of alkaloids, seven types of coumarins, and 60 kinds of flavonoids. This established method can quickly and efficiently identify chemical constituents in Duzhong Jiangya tablet, lay a foundation for the research on the efficacy and quality of this traditional Chinese medicine, and provide a reference for the characterization of the chemical constituents of other traditional Chinese medicine preparations. K E Y W O R D S chemical composition, Duzhong Jiangya Tablet, Fourier transform ion cyclotron resonance, traditional Chinese medicine 1 INTRODUCTION Varieties of medicinal materials in traditional Chinese medicine compound preparations have complex chemical components. The synergy between the chemical components is the material basis for the traditional Chinese medicine preparations to exert their pharmaco-Article Related Abbreviations: ICR, ion cyclotron resonance logical effects [1]. The active ingredients of most Chinese medicines are still unknown, although there are many reports on Chinese medicine research. Therefore, it is very important to establish a fast and efficient method to identify the chemical components in traditional Chinese medicine. This can reasonably explain the material basis of the efficacy of traditional Chinese medicine. Duzhong Jiangya Tablet consists of Eucommia ulmoides Oliv, Leonurus japonicus Houtt, Prunella vulgaris L,

Evaluation of Biological Mechanisms of Quanduzhong Capsule for Treating Osteoporosis by Integrating Untargeted Metabolomics and Network Pharmacology

“…Most of the DZ compounds were collected from references. 9,10,[12][13][14][15][16][17][18] The compound library contains compounds' names, molecular formulas, accurate molecular weights, and structures. We confirmed the structures using online databases such as PubMed, PubChem, CNKI, Science Direct, Springer Link, and Drugfuture.…”