Rhoifolin loaded in PLGA nanoparticles alleviates oxidative stress and inflammation <i>in vitro</i> and <i>in vivo</i>

Al‐Shalabi, Eveen; Abusulieh, Samah; Hammad, Alaa M.; Sunoqrot, Suhair

doi:10.1039/d2bm00309k

Cited by 21 publications

(15 citation statements)

References 64 publications

(90 reference statements)

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“…In brief, freshly prepared AmB@pQCT-PEG and AmB@pQCT NPs were each drop-cast on precleaned TiO 2 -coated Si wafers. The wafers were dried under vacuum and analyzed using a Kratos Axis SUPRA instrument fitted with a monochromated Al kα X-ray source (1486.7 eV) as previously described …”

Section: Methodsmentioning

confidence: 99%

Amphotericin B-Loaded Plant-Inspired Polyphenol Nanoparticles Enhance Its Antifungal Activity and Biocompatibility

Sunoqrot

Al‐Bakri

Ibrahim

et al. 2022

ACS Appl. Bio Mater.

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Amphotericin B (AmB) is one of the first-line treatments for systemic fungal infections, yet it suffers from doselimiting systemic toxicity and high cost of less toxic lipid-based formulations. Here, we report on a facile approach to synthesize an AmB-loaded nanomedicine by leveraging plant-inspired oxidative self-polymerization of the ubiquitous polyphenol quercetin (QCT). Polymerized QCT nanoparticles (pQCT NPs) were formed, loaded with AmB, and functionalized with poly(ethylene glycol) (PEG) to impart steric stability in a simple procedure that relied on mixing followed by dialysis. The AmB-loaded NPs (AmB@pQCT-PEG NPs) were characterized by a drug loading efficiency of more than 90%, a particle size of around 160 nm, a polydispersity index of 0.07, and a partially negative surface charge. AmB release from the NPs was sustained over several days and followed the Korsmeyer−Peppas model with a release exponent (n) value >0.85, denoting drug release by polymer relaxation and swelling. A hemolysis assay revealed the NPs to be highly biocompatible, with negligible hemolytic activity and 30−60% hemolysis after 1 and 24 h of incubation with erythrocytes, respectively, across a wide concentration range (6.25−100.00 μg/mL). Conversely, equivalent concentrations of free AmB caused 90−100% hemolysis within the same timeframe. Importantly, AmB@pQCT-PEG NPs outperformed free AmB in microbial susceptibility assays on Candida albicans, achieving a minimum inhibitory concentration of 62.5 ng/mL after 48 h of incubation, which was 2-fold lower than the free drug. Our results demonstrate that pQCT NPs may serve as a viable AmB delivery platform for the treatment of fungal infections and potentially other AmB-susceptible pathogens.

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Section: Methodsmentioning

confidence: 99%

Amphotericin B-Loaded Plant-Inspired Polyphenol Nanoparticles Enhance Its Antifungal Activity and Biocompatibility

Sunoqrot

Al‐Bakri

Ibrahim

et al. 2022

ACS Appl. Bio Mater.

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“…PLGA nanospheres for instance are phagocytosed by DCs, and when loaded with antitumor peptides, they trigger a significant specific cytotoxic T cells anticancer response at a lower concentration compared to peptides administered through incomplete Freund’s adjuvant . Nanoprecipitation, emulsification, and self-assembly are among the commonly applied methods for the preparation of PNPs. ,− While nanoprecipitation (Figure A) is characterized by its simplicity and reproducibility, it is a troublesome technique to optimize since the adjustment of solvent, cargo, and polymer ratios is critical . Emulsification (Figure B) allows for the loading of both hydrophilic and hydrophobic cargos, yet the entrapment efficiency is greatly limited .…”

Section: Polymer-based Nps As Cancer Vaccine Platformsmentioning

confidence: 99%

Lipid- and Polymer-Based Nanocarrier Platforms for Cancer Vaccine Delivery

Sunoqrot,

Abdel Gaber,

Abujaber

et al. 2024

ACS Appl. Bio Mater.

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Cancer immunotherapy has gained popularity in recent years in the search for effective treatment modalities for various malignancies, particularly those that are resistant to conventional chemo-and radiation therapy. Cancer vaccines target the cancer-immunity cycle by boosting the patient's own immune system to recognize and kill cancer cells, thus serving as both preventative and curative therapeutic tools. Among the different types of cancer vaccines, those based on nanotechnology have shown great promise in advancing the field of cancer immunotherapy. Lipid-based nanoparticles (NPs) have become the most advanced platforms for cancer vaccine delivery, but polymer-based NPs have also received considerable interest. This Review aims to provide an overview of the nanotechnologyenabled cancer vaccine landscape, focusing on recent advances in lipid-and polymer-based nanovaccines and their hybrid structures and discussing the challenges against the clinical translation of these important nanomedicines.

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“…12 To improve the biocompatibility, targeting and pharmacological effects of TPs, some kinds of nanoformulations to load TPs have been synthesized and they have demonstrated good biosafety in vivo. [13][14][15][16] In this work, to improve the bioavailability and facilitate the delivery of TPs to the inflammatory sites, poly(lactic-co-glycolic acid) (PLGA) nanoparticles 17 were employed to load TPs to form TP-NPs. Then, platelet membranes (PMs) were isolated from the blood of healthy mice and coated onto the TP-NPs to endow PM@TP-NPs with inflammation targeting properties.…”

Section: Introductionmentioning

confidence: 99%

“…In this work, to improve the bioavailability and facilitate the delivery of TPs to the inflammatory sites, poly(lactic- co -glycolic acid) (PLGA) nanoparticles 17 were employed to load TPs to form TP-NPs. Then, platelet membranes (PMs) were isolated from the blood of healthy mice and coated onto the TP-NPs to endow PM@TP-NPs with inflammation targeting properties.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic effects of tea polyphenol-loaded nanoparticles coated with platelet membranes on LPS-induced lung injury

Hua¹,

Zhao²,

Yao³

et al. 2023

Biomater. Sci.

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Rhoifolin loaded in PLGA nanoparticles alleviates oxidative stress and inflammation in vitro and in vivo

Abstract: Rhoifolin (ROF) is a bioactive plant flavonoid with potent antioxidant and anti-inflammatory activity. However, no delivery system has yet been developed for ROF to overcome its biopharmaceutical limitations. The purpose...

Cited by 21 publications

References 64 publications

Amphotericin B-Loaded Plant-Inspired Polyphenol Nanoparticles Enhance Its Antifungal Activity and Biocompatibility

Amphotericin B-Loaded Plant-Inspired Polyphenol Nanoparticles Enhance Its Antifungal Activity and Biocompatibility

Lipid- and Polymer-Based Nanocarrier Platforms for Cancer Vaccine Delivery

Therapeutic effects of tea polyphenol-loaded nanoparticles coated with platelet membranes on LPS-induced lung injury

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