Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic

Singh, Purnima; Singh, Shiv Mohan; Tsuji, Masaharu; Prasad, Gandham S.; Hashizume, Tamotsu

doi:10.1016/j.cryobiol.2014.01.006

Cited by 38 publications

(43 citation statements)

References 37 publications

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“…(Iefuji et al 1994), M. blollopis (Tsuji et al 2013a), R. svalbardensis sp. nov (Singh et al 2014), Tetracladium setigerum (Abdullah 1989) and species of Cryptoccoccus, Leuconeurospora, Dioszegia, and Rhodotorula (Carrasco et al 2012). An amylase originally described in Cr.…”

Section: Extracellular Hydrolytic Enzymesmentioning

confidence: 98%

“…In one study looking at a freezeing-tolerant Rhodotorula svalbardensis sp. nov. isolated from Arctic cryoconite holes at Ny-Alesund, the presence of AFPs was suggested by the formation of hexagonal ice crystal structures in broth culture (Singh et al 2014). High TH and inhibition of ice recrystallization properties were detected in culture filtrates of the psychrophilic yeast Glaciozyma antarctica and, according to genomic data, a cDNA encoding a probable 177-residue AFP was found.…”

Section: Antifreeze Proteinsmentioning

confidence: 98%

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Physiological adaptations of yeasts living in cold environments and their potential applications

Alcaı́no

Cifuentes

Baeza

2015

World J Microbiol Biotechnol

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Yeasts, widely distributed across the Earth, have successfully colonized cold environments despite their adverse conditions for life. Lower eukaryotes play important ecological roles, contributing to nutrient recycling and organic matter mineralization. Yeasts have developed physiological adaptations to optimize their metabolism in low-temperature environments, which affect the rates of biochemical reactions and membrane fluidity. Decreased saturation of fatty acids helps maintain membrane fluidity at low temperatures and the production of compounds that inhibit ice crystallization, such as antifreeze proteins, helps microorganisms survive at temperatures around the freezing point of water. Furthermore, the production of hydrolytic extracellular enzymes active at low temperatures allows consumption of available carbon sources. Beyond their ecological importance, interest in psychrophilic yeasts has increased because of their biotechnological potential and industrial uses. Long-chain polyunsaturated fatty acids have beneficial effects on human health, and antifreeze proteins are attractive for food industries to maintain texture in food preserved at low temperatures. Furthermore, extracellular cold-active enzymes display unusual substrate specificities with higher catalytic efficiency at low temperatures than their mesophilic counterparts, making them attractive for industrial processes requiring high enzymatic activity at low temperatures. In this minireview, we describe the physiological adaptations of several psychrophilic yeasts and their possible biotechnological applications.

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Section: Extracellular Hydrolytic Enzymesmentioning

confidence: 98%

Section: Antifreeze Proteinsmentioning

confidence: 98%

Physiological adaptations of yeasts living in cold environments and their potential applications

Alcaı́no

Cifuentes

Baeza

2015

World J Microbiol Biotechnol

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“…Recently, characterised yeast and filamentous fungi from cryoconite holes of Midre Lovénbreen glacier, Svalbard. Further, and subsequently, a novel species Rhodotorula svalbardensis was reported from cryconites (Singh and others 2014). Edwards and others (2013) analysed fungi from cryoconites of Austre Brøggerbreen (AB), Midre Lovénbreen (ML) and Vestre Brøggerbreen (VB) glaciers with terminalrestriction fragment length polymorphism (T-RFLP) profiles, and recorded the presence of Articulospora and Varicosporium through a cultured approach.…”

Section: Introductionmentioning

confidence: 99%

Characterisation of yeast and filamentous fungi from Brøggerbreen glaciers, Svalbard

Singh¹,

Roy²,

Tsuji

2016

Polar Record

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ABSTRACT. Cryoconite holes have ecological and biotechnological importance. This article presents results on culturable cryophilic yeasts and filamentous fungi isolated from cryoconite holes at Austre and Vestre Brøggerbreen glaciers, Svalbard. Based on DNA sequence data, these were identified as Rhodotorula sp., Thelebolus sp., and Articulospora tetracladia. Amongst these, Articulospora tetracladia (88.7-89.4% gene similarity with 5.8S rDNA) is a novel species, yet to be described. Filamentous fungus Articulospora sp. Cry-FB1 and Cry-FB2, expressed high amylase, cellulase, lipase and protease activities while yeast Rhodotorula sp. Cry-FB3 showed high amylase and cellulase activity. Thelebolus sp. Cry-YB 240 and Cry-YB 241 showed protease and urease activities. The effects of temperature, and salt on the growth of the cultures were studied. Optimum temperature of growth was on 10ºC at pH 7.0. Filamentous fungi and yeast in the cryoconite holes possibly drive the process of organic macromolecule degradation through cold-adapted enzyme secretion, thereby assisting in nutrient cycling in these supraglacial environments. Further, these cryophilic fungi, due to their enzyme producing ability, may provide an opportunity for biotechnological research in the Arctic.

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“…Segundo Ferrero et al (1996) (TURKIEWICZ et al, 2003;VAZ et al, 2011;DUARTE et al, 2013;SINGH et al, 2014).…”

Section: Pseudomonas Fragiunclassified

“…Também uma nova espécie deste gênero, Rhodotorula svalbardensis, isolada no Ártico, foi recentemente estudada quanto à produção de proteases, exibindo boa atividade enzimática a 4°C (SINGH et al, 2014).…”

Section: Rhodotorula Mucilaginosaunclassified

Estabelecimento de condições de cultivo de leveduras isoladas na Antártica visando à produção de proteases

Chaud¹

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Rhodotorula svalbardensis sp. nov., a novel yeast species isolated from cryoconite holes of Ny-Ålesund, Arctic

Cited by 38 publications

References 37 publications

Physiological adaptations of yeasts living in cold environments and their potential applications

Physiological adaptations of yeasts living in cold environments and their potential applications

Characterisation of yeast and filamentous fungi from Brøggerbreen glaciers, Svalbard

Estabelecimento de condições de cultivo de leveduras isoladas na Antártica visando à produção de proteases

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