Characterisation of yeast and filamentous fungi from Brøggerbreen glaciers, Svalbard

Singh, Purnima; Roy, Utpal; Tsuji, Masaharu

doi:10.1017/s0032247416000085

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…Its presence was observed in close association with clumps of dead and alive ice algae, kept together by algal EPS, suggesting a potential role in the degradation of the ice algae. Articulospora has been previously described in cryoconite holes in Svalbard (Singh and Singh, 2012; Edwards et al, 2013; Singh et al, 2016) and its role as an important carbon decomposer was hypothesized. This result was confirmed by amplicon sequencing ( Cladochasiella divergens ; 0.11–0.15%) that furthermore showed the presence of the plant-associated black yeast genus Cladophialophora (9.2–12.6%).…”

Section: Discussionmentioning

confidence: 99%

Darkening of the Greenland Ice Sheet: Fungal Abundance and Diversity Are Associated With Algal Bloom

et al. 2019

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Recent studies have highlighted the importance of ice-algal blooms in driving darkening and therefore surface melt of the Greenland Ice Sheet (GrIS). However, the contribution of fungal and bacterial communities to this microbially driven albedo reduction remains unconstrained. To address this significant knowledge gap, fungi were isolated from key GrIS surface habitats (surface ice containing varying abundance of ice algae, supraglacial water, cryoconite holes, and snow), and a combination of cultivation and sequencing methods utilized to characterize the algal-associated fungal and bacterial diversity and abundance. Six hundred and ninety-seven taxa of fungi were obtained by amplicon sequencing and more than 200 fungal cultures belonging to 46 different species were isolated through cultivation approaches. Basidiomycota dominated in surface ice and water samples, and Ascomycota in snow samples. Amplicon sequencing revealed that bacteria were characterized by a higher diversity (883 taxa detected). Results from cultivation as well as ergosterol analyses suggested that surface ice dominated by ice algae and cryoconite holes supported the highest fungal biomass (10 4 –10 5 CFU/100 ml) and that many fungal taxa recognized as endophytes and plant pathogens were associated with dark ice characterized by a high abundance of ice algae. This paper significantly advances this field of research by investigating for the first time the fungal abundance and diversity associated with algal blooms causing the darkening of the GrIS. There is a strong association between the abundance and diversity of fungal species and the blooming of algae on the surface ice of the Greenland Ice Sheet.

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Section: Discussionmentioning

confidence: 99%

Darkening of the Greenland Ice Sheet: Fungal Abundance and Diversity Are Associated With Algal Bloom

et al. 2019

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“…Studies on Arctic cryoconite began with the investigation of brown algae [26]. Afterward, cyanobacteria [27,28], viruses and virus-like particles [29,30], diatoms [31], Bacteria [32][33][34], yeast and filamentous fungi [35][36][37][38] were focused on in Svalbard. Cryoconite holes have also been searched from the Greenland Ice Sheet (GrIS) [39,40] and Himalayan glaciers [11].…”

Section: Introductionmentioning

confidence: 99%

Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic

et al. 2020

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To understand the microbial composition and diversity patterns, cryoconite granules were collected from two geographical areas, i.e., Nepali Himalaya and Greenland, Arctic. 16S rRNA, ITS and the D1/D2 domain sequencing techniques were used for characterization of microbial communities of the four glaciers. The total 13 species of bacteria such as Bacillus aryabhattai, Bacillus simplex, Brevundimonas vesicularis, Cryobacterium luteum, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Glaciihabitans tibetensis, Leifsonia kafniensis, Paracoccus limosus, Polaromonas glacialis, Sporosarcina globispora, Staphylococcus saprophyticus, Variovorax ginsengisoli, and 4 species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Dothideomycetes sp., Helotiales sp. were recorded from Nepali Himalaya. Among these, 12 species of bacteria and 4 species of fungi are new contributions to Himalaya. In contrast to this, six species of bacteria such as Bacillus cereus, Cryobacterium psychrotolerans, Dermacoccus nishinomiyaensis, Enhydrobacter aerosaccus, Glaciihabitans tibetensis, Subtercola frigoramans, and nine species of fungi such as Goffeauzyma gilvescens, Mrakia robertii, Naganishia vaughanmartiniae, Piskurozyma fildesensis, Rhodotorula svalbardensis, Alatospora acuminata, Articulospora sp., Phialophora sp., Thelebolus microspores, and Dothideomycetes sp.), were recorded from Qaanaaq, Isunnguata Sermia and Thule glaciers, Greenland. Among these, five species of bacteria and seven species of fungi are new contributions to Greenland cryoconite. Microbial analyses indicate that the Nepali Himalayan cryoconite colonize higher numbers of microbial species compared to the Greenland cryoconite.

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“…In glacier cryoconite ecosystems, microbes play an important role [11,12], but the Himalayan glacier cryoconites and their ecosystem still represent a gap in investigations. In these natural ecosystems, microorganisms able to survive in low temperatures are widespread, where they often represent the dominant flora, and as a result, they should be regarded as the most flourishing colonizers of our planet [13].…”

Section: Introductionmentioning

confidence: 99%

Identification of Fungal Communities Isolated from Himalayan Glacier Cryoconites

2022

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The current study focuses on fungi that were isolated from cryoconite holes of the Hamtah glacier in the Himalayas. Cryoconite holes have ecological and biotechnological importance. A total of seven cryoconite samples were collected from different locations and subjected to the isolation of psychotropic fungi at 1, 4, 15 and 22 °C. Isolates were identified by ITS and D1/D2 region sequences. The result showed culturable yeasts (45) and filamentous fungi (10) belonging to four ascomycetous classes (Dothideomycetes, Eurotiomycetes, Saccharomycetes and Sordariomycetes) and two basidiomycetes’ classes (Microbotryomycetes and Tremellomycetes). Physiological characteristics such as the pH, temperature, salt tolerance, carbon source utilization and antibiotics sensitivity of the isolates were studied. All the isolates were grown from acidic to alkaline pH and were able to grow at 1 to 22 °C. The fungal cultures isolated were screened to produce cold active enzymes such as amylase, cellulase, lipase, protease and catalase. Cellulase activity was detected at its maximum at both 4 and 15 °C. Himalayan cryoconites fungi showed immense potential for biotechnological and industrial applications. To the best of our knowledge, this is the first record of the characterization of fungal communities present in the glacier cryoconites of the Himalayas.

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Characterisation of yeast and filamentous fungi from Brøggerbreen glaciers, Svalbard

Cited by 11 publications

References 33 publications

Darkening of the Greenland Ice Sheet: Fungal Abundance and Diversity Are Associated With Algal Bloom

Darkening of the Greenland Ice Sheet: Fungal Abundance and Diversity Are Associated With Algal Bloom

Contrasting Patterns of Microbial Communities in Glacier Cryoconite of Nepali Himalaya and Greenland, Arctic

Identification of Fungal Communities Isolated from Himalayan Glacier Cryoconites

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