Rhodotorula bloemfonteinensis sp. nov., Rhodotorula eucalyptica sp. nov., Rhodotorula orientis sp. nov. and Rhodotorula pini sp. nov., yeasts isolated from monoterpene-rich environments

Abstract

Recent rDNA sequencing of 25 isolates from a previous study, during which limonene-utilizing yeasts were isolated from monoterpene-rich environments by using 1,4-disubstituted cyclohexanes as sole carbon sources, led to the identification of four hitherto unknown Rhodotorula species. Analyses of the 26S rDNA D1/D2 region as well as the internal transcribed spacer (ITS) domain indicated that two isolates (CBS 8499T and CBS 10736) were identical and were closely related to Rhodotorula cycloclastica, a previously described limonene-utilizing yeast. These novel isolates differed from known yeast species and could be distinguished from R. cycloclastica by standard physiological tests. The other three isolates represent three novel Rhodotorula species, closely related to Sporobolomyces magnisporus. These three species could also be distinguished from other Rhodotorula species by standard physiological tests. Based on these results, we suggest that the new isolates represent novel species, for which the names Rhodotorula eucalyptica sp. nov. (type strain CBS 8499T = NRRL Y-48408T), Rhodotorula pini sp. nov. (type strain CBS 10735T = NRRL Y-48410T), Rhodotorula bloemfonteinensis sp. nov. (type strain CBS 8598T = NRRL Y-48407T) and Rhodotorula orientis sp. nov. (type strain CBS 8594T = NRRL Y-48719T) are proposed. R. eucalyptica and R. pini can also utilize limonene.

Phylogeny of yeasts and related filamentous fungi within Pucciniomycotina determined from multigene sequence analyses

“…(2011) . The close relationship of the magnisporus clade with the Erythrobasidiales was shown in Pohl et al. (2011) and in the rDNA genes-based tree in this study ( Fig.…”

“…The magnisporus clade consisted of Sporobolomyces magnisporus and three Rhodotorula species described recently by Pohl et al. (2011) .…”

The yeast lichenosphere: high diversity of basidiomycetes from the lichens Tephromela atra and Rhizoplaca melanophthalma

“…Instead, the two strains coming from R. melanophthalma thalli collected on the Alps are nested within Colacogloea spp. These yeasts were isolated from soil and plant residues in monoterpene-rich environments (Thanh et al, 2004;Pohl et al, 2011) and beech forest soils (Yurkov et al, 2016;Kachalkin et al, 2019), two environments completely different from those visited by us. However, some Colacogloea spp.…”