Rhodococcus enclensis sp. nov., a novel member of the genus Rhodococcus

Dastager, Syed G.; Mawlankar, Rahul; Tang, Shan-Kun; Krishnamurthi, Srinivasan; Ramana, V. Venkata; Joseph, Neeta; Shouche, Yogesh S.

doi:10.1099/ijs.0.061390-0

Cited by 18 publications

(9 citation statements)

References 44 publications

(31 reference statements)

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“…These major fatty acids were previously reported in species of the genus Rhodococcus Jones & Goodfellow, 2012;Nimaichand et al, 2013;K€ ampfer et al, 2013;Dastager et al, 2014).…”

Section: Strains Uc12supporting

confidence: 76%

Rhodococcus pedocola sp. nov. and Rhodococcus humicola sp. nov., two antibiotic-producing actinomycetes isolated from soil

Nguyen

Kim

2016

International Journal of Systematic and Evolutionary Microbiology

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Two novel actinobacterial strains, UC12T and UC33 Both strains showed similar chemotaxonomic characteristics. The major fatty acids were C 16 : 0 , summed feature 3, C 18 : 1 !9c and C 18 : 0 10-methyl. The polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and phosphatidylinositol mannoside. The major quinone derived was MK-8(H 2 ). The cell-wall peptidoglycan contained meso-diaminopimelic acid, and galactose, glucose, arabinose and ribose were detected in whole cells. Mycolic acids were detected. The DNA G+C content of strains UC12 T and UC33 T was 72.7 mol% and 68.8 mol%,respectively. Both strains produced antibiotic(s) that inhibited bacterial pathogens but not fungi. Based on the physiological, biochemical and genotypic features and the DNA-DNA hybridization between the isolates and type strains of closely related species, we propose that these bacteria be classified as novel species of the genus Rhodococcus with the names Rhodococcus pedocola sp. nov. (type strain UC12 T =KACC 18499

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“…These major fatty acids were previously reported in species of the genus Rhodococcus Jones & Goodfellow, 2012;Nimaichand et al, 2013;K€ ampfer et al, 2013;Dastager et al, 2014).…”

Section: Strains Uc12supporting

confidence: 76%

Rhodococcus pedocola sp. nov. and Rhodococcus humicola sp. nov., two antibiotic-producing actinomycetes isolated from soil

Nguyen

Kim

2016

International Journal of Systematic and Evolutionary Microbiology

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“…The description is as before (Dastager et al, 2014a ) with the following modification. The G+C content of the type-strain genome is 62.3%, its approximate size 7.46 Mbp, its GenBank deposit SAMN04263893 .…”

Section: Discussionmentioning

confidence: 99%

Genome-Based Taxonomic Classification of the Phylum Actinobacteria

et al. 2018

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The application of phylogenetic taxonomic procedures led to improvements in the classification of bacteria assigned to the phylum Actinobacteria but even so there remains a need to further clarify relationships within a taxon that encompasses organisms of agricultural, biotechnological, clinical, and ecological importance. Classification of the morphologically diverse bacteria belonging to this large phylum based on a limited number of features has proved to be difficult, not least when taxonomic decisions rested heavily on interpretation of poorly resolved 16S rRNA gene trees. Here, draft genome sequences of a large collection of actinobacterial type strains were used to infer phylogenetic trees from genome-scale data using principles drawn from phylogenetic systematics. The majority of taxa were found to be monophyletic but several orders, families, and genera, as well as many species and a few subspecies were shown to be in need of revision leading to proposals for the recognition of 2 orders, 10 families, and 17 genera, as well as the transfer of over 100 species to other genera. In addition, emended descriptions are given for many species mainly involving the addition of data on genome size and DNA G+C content, the former can be considered to be a valuable taxonomic marker in actinobacterial systematics. Many of the incongruities detected when the results of the present study were compared with existing classifications had been recognized from 16S rRNA gene trees though whole-genome phylogenies proved to be much better resolved. The few significant incongruities found between 16S/23S rRNA and whole genome trees underline the pitfalls inherent in phylogenies based upon single gene sequences. Similarly good congruence was found between the discontinuous distribution of phenotypic properties and taxa delineated in the phylogenetic trees though diverse non-monophyletic taxa appeared to be based on the use of plesiomorphic character states as diagnostic features.

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“…The average contig size was 61,133 bp, and the N 50 contig size was 158,305 bp. The size of the assembled genome is 7,213,744 bp, with a GC content of 62.3% (this is a low value for R. enclensis , for which the GC content is 66.9% [ 8 ], but it is in the range of those obtained for Rhodococcus species in general, at 62 to 69% [ 9 ]). The RAST annotation server was used for annotating the draft genome of Rhodococcus enclensis 23b-28 ( http://rast.nmpdr.org ).…”

Section: Genome Announcementmentioning

confidence: 99%