An actinobacterial strain was isolated from a sediment sample from Chorao Island, in Goa province, India, and subjected to a taxonomic investigation. The isolate, designated NIO-1021 T , was a Gram-stain positive, aerobic, non-motile and coccoid. Strain NIO-1021 T was identified as a member of the genus Kocuria by a polyphasic approach. Strain NIO-1021 T could be differentiated from other members of the genus Kocuria on the basis of physiology and 16S rRNA gene sequence analysis. The 16S rRNA gene sequence similarity of strain NIO-1021 T to the most closely related species, Kocuria marina KCTC 9943 T , was 98.6 % with 19 nt differences). Furthermore, DNA-DNA hybridization analysis revealed that the novel strain had lower relatedness with the type strains of other members of the genus Kocuria. The strain formed a monophyletic clade with K. marina with 100 % bootstrap values. The major phospholipids were phosphatidylglycerol, diphosphatidylglycerol and two unidentified lipids. The predominant menaquinone was MK-7(H 2 ). The major fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . The DNA G+C content of strain NIO-1021 T was 60.5 mol%. Chemotaxonomic and phylogenetic properties of the strain were consistent with its classification as representing a member of the genus Kocuria. On the basis of phenotypic, chemotypic and molecular characteristics, strain NIO-1021 T is considered to represent a novel species of the genus Kocuria, for which the name Kocuria indica sp. nov. is proposed, with strain NIO-1021 T (5NCIM 5455 T 5DSM 25126 T 5CCTCC AA 209050 T ) as the type strain.The genus Kocuria was first proposed by Stackebrandt et al. (1995) on the basis of a detailed phylogenetic and chemotaxonomic analysis of the genus Micrococcus (Stackebrandt et al., 1995). This led further to the description of the genera Dermacoccus, Kocuria, Kytococcus and Nesterenkonia. The genus Kocuria embraced three species formerly classified as members of the genus Micrococcus, i.e. Kocuria rosea (Micrococcus roseus; Flügge 1886), Kocuria varians (Micrococcus varians; Migula, 1900) and Kocuria kristinae (Micrococcus kristinae; Kloos et al., 1974). Based upon 16S rRNA gene sequence similarities the genus Kocuria represents one of the deepest lineages in the family Stackebrandt et al., 1995). The third clade includes only Kocuria palustris (Kovács et al., 1999). The last clade appears to be the deepest lineage in the family and comprises Kocuria atrinae (Park et al., 2010b) During an investigation exploring actinobacterial diversity in the marine environment of Chorao Island of Goa province, India, a coccoid actinobacterial strain was isolated and was subjected to a polyphasic investigation to determine its taxonomic position.Strain NIO-1021 T was isolated from a marine sediment sample taken from Chorao Island. After primary isolation and purification on marine agar 2216 (Difco) at 28 u C for 2 weeks, the purified strain was subcultured on the same medium and stored as slants at 4 u C and as 20 % (v/v) glycerol suspension...
A Gram-stain-positive bacterium, designated strain NIO-1109 T , was isolated from a marine sediment sample from Chorao Island, Goa, India.
and galactose and arabinose as the cell wall sugars. The major fatty acids were C 16 : 0 , C 18 : 1 v9c, C 16 : 1 (v6c and/or v7c) and 10-methyl C 18 : 0 . The only menaquinone detected was MK-8(H 2 ), while the major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannoside and one unknown phospholipid. The G+C content of the genomic DNA was 66.9 mol%. The phenotypic and genotypic data showed that strain NIO-1009 T warrants recognition as a novel species of the genus Rhodococcus for which the name Rhodococcus enclensis sp. nov., is proposed; the type strain is NIO-1009 T (5NCIM 5452 T 5DSM 45688 T ).
A novel bacterial strain, designated SGD-1123(T) was isolated from Chorao Island, in Goa Province, India. The strain was found to be able to grow at 15-42 °C, pH 5-12 and 0-12 % (w/v) NaCl. The whole cell hydrolysates were found to contain meso-diaminopimelic acid, galactose and arabinose. The major fatty acids were identified as iso-C15:0 and anteiso-C15:0, MK-7 was identified as the predominant menaquinone and the predominant polar lipids were identified as diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified aminolipid. The genomic DNA G+C content was determined to be 44.6 mol%. Phylogenetic analysis based on 16S rRNA gene sequences placed the isolate within the genus Bacillus and further revealed that strain SGD-1123(T) had highest sequence similarity with Bacillus aquimaris, and forms a separate clade with its closest relatives i.e. B. aquimaris, Bacillus vietnamensis and Bacillus marisflavi, with which it shares 94.5, 94.1 and 94.1 % similarity respectively. The phylogenetic, chemotaxonomic and phenotypic analyses indicated that strain SGD-1123(T) represents a novel species within the genus Bacillus, for which the name Bacillus enclensis is proposed. The type strain is SGD-1123(T) (NCIM 5450(T)=CCTCC AB 2011125(T)).
A novel Gram-positive strain, designated NIO-1003(T), was isolated from a marine sediment sample collected from the Chorao Island, Goa Provence, India. Strain NIO-1003(T) was found to be strictly aerobic, motile, endospore-forming rods. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NIO-1003(T) belongs to the genus Fictibacillus and to be most closely related to Fictibacillus rigui KCTC 13278(T), Fictibacillus solisalsi KCTC 13181(T) and Fictibacillus barbaricus DSM 14730(T) with 98.2, 98.0 and 97.2 % similarity and 25, 28, 39 nucleotide differences respectively. Strain NIO-1003(T) was characterized by having cell-wall peptidoglycan based on meso-diaminopimelic acid and MK-7 as the predominant menaquinone. The polar lipid profile exhibited the major compounds diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. In addition, minor amounts of an aminophospholipid were detected. The major fatty acids were identified as ai-C15:0, iso-C15:0, ai-C17:0 and C16:0, supporting the grouping of strain NIO-1003(T) into the family Bacillaceae. The DNA G+C content of strain NIO-1003(T) was determined to be 42.6 mol%. On the basis of phenotypic properties, phylogeny and DNA-DNA hybridisation analysis, strain NIO-1003(T) is considered to represent a novel species of the genus Fictibacillus for which the name Fictibacillus enclensis sp. nov. is proposed. The type strain is NIO-1003(T) (= NCIM 5458(T) = DSM 25142(T)).
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