Rhodiola rosea L. (Roseroot): In Vitro Regeneration and the Biological Activity of Roots

Furmanowa, M.; Olȩdzka, H.; Michalska, M.; Sokolnicka, I; Radomska, Dominika

doi:10.1007/978-3-662-08612-4_23

Cited by 17 publications

(21 citation statements)

References 10 publications

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“…The choice of the explant type is also crucial. Leaf disks [6,7,17], stem segments [7], shoot tips and buds [8] and nodes and rhizome buds [18], from plants taken from their natural environment were used with inconsistent results in experiments with other Rhodiola species. In our initial experiments, the five types of wild plant tissue explants (apical and rhizome buds, stem and rhizome segments, leaf nodes) formed green or pale callus, new shoots, plantlets or leaf rosettes.…”

Section: Discussionmentioning

confidence: 99%

“…When information about a species and/or specificity of the genotype/ecotype is limited, it is a good idea to use of large number of culture media and plant material to ensure success. Furmanowa et al [6] tested media recipes of Murashige & Skoog, Linsmaer & Skoog, Gamborg, White and Nitsch & Nitsch to find that the last was the best for plant development from shoot tips, while MS media proved to be better than Litvay or White media for the evaluation of the morphogenic potential of R. rosea and R. iremelica [8]. It was also better for callus culture development and regeneration in Tibetan R. rosea [7].…”

Section: Discussionmentioning

confidence: 99%

“…The first attempt at initiating an in vitro culture occurred 20 years ago [5] and was followed by a few other reports. These reports refer to the effect of different culture media and of various types of explants on ability for callogenesis, organogenesis and regeneration of R. rosea, as well as other factors influencing the induction of differentiated and undifferentiated growth in vitro [6][7][8]. Some supplements, like roseroot rhizome crude extracts, could stimulate shoot formation of R. rosea and R. iremelica when added to the media [9].…”

Section: Introductionmentioning

confidence: 99%

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Bulgarian golden root in vitro cultures for micropropagation and reintroduction

Tasheva

Kosturkova

2010

Open Life Sciences

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Abstract:Rhodiola rosea is an endangered medicinal plant used for cancer, cardiovascular, and nervous system diseases therapy. Due to its limited distribution and sustainability alternative methods for production of its valuable substances are under investigation. Using in vitro techniques apical and rhizome buds, leaf nodes, stem and radix segments from wild plants and in vitro seedlings were plated on 24 modified Murashige and Skoog (1962) media. Decontamination of plant material was successful only in 21% of the schemes. The best shoot induction was obtained from seedling explants on media containing 2 mg/l zeatin or N 6 -benzylaminopurine, each. Their reduction stimulated shoot formation in the next passages (multiplication rate up to 5). Efficient rooting was induced on half-strength MS with 2 mg/l Indole-3-butyric acid and stimulated by adding 0.2 mg/l Indolyl-3-acetic acid. Regenerants rooted in perlite, peat, and soil (1:1:2), adapted in greenhouse, and transplanted in the mountains survived (70%) and developed like the wild plants. Salidroside content of these plants after one or two years was high (0.64 and 0.61% in rhizomes and 0.62 and 0.53% in roots, respectively). This is the first established efficient scheme for micropropagation of Bulgarian R. rosea allowing habitats restoration, germplasm conservation, and potential application of biotechnology for production of valuable substances.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bulgarian golden root in vitro cultures for micropropagation and reintroduction

Tasheva

Kosturkova

2010

Open Life Sciences

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“…Coumarins, lactones (Furmanowa et al 1995) and phenolic acids: chlorogenic-, hydroxycinnamic-and gallic acid (Brown et al 2002) were also found in R. rosea. Lotaustralin was isolated from the roots (Akgul et al 2004).…”

Section: Phytochemical Composition Of Rhodiola Roseamentioning

confidence: 99%

“…Lotaustralin was isolated from the roots (Akgul et al 2004). Today, rosin, rosavin, rosarin and salidroside are considered as diagnostic marker compounds of R. rosea, all demonstrating adaptogenic activity (Furmanowa et al 1995;Germano et al 1999;Panossian and Wagner 2005).…”

Section: Phytochemical Composition Of Rhodiola Roseamentioning

confidence: 99%

Rhodiola rosea L.: from golden root to green cell factories

et al. 2016

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LC/MS/MS identiﬁcation of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggregates

Tolonen¹,

György²,

Jalonen³

et al. 2004

Biomedical Chromatography

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Cinnamyl alcohol was added to the media of compact callus aggregates (CCA) of Rhodiola rosea for stimulating the production of cinnamyl glycosides. The biotransformation reaction produced high amounts of rosin, while only a very low amount of rosavin was produced. As the consumption rate of cinnamyl alcohol was much higher than production of rosin, the aqueous methanol extracts of compact callus aggregates were studied by liquid chromatography-mass spectrometric methods and four new unexpected biotransformation products of cinnamyl alcohol were identified.

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Rhodiola rosea L. (Roseroot): In Vitro Regeneration and the Biological Activity of Roots

Cited by 17 publications

References 10 publications

Bulgarian golden root in vitro cultures for micropropagation and reintroduction

Bulgarian golden root in vitro cultures for micropropagation and reintroduction

Rhodiola rosea L.: from golden root to green cell factories

LC/MS/MS identiﬁcation of glycosides produced by biotransformation of cinnamyl alcohol in Rhodiola rosea compact callus aggregates

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