Rhodanese Rdl2 produces reactive sulfur species to protect mitochondria from reactive oxygen species

Wang, Qingda; Chen, Zhigang; Zhang, Xi; Xin, Yuping; Xia, Yongzhen; Xun, Luying; Liu, Huaiwei

doi:10.1016/j.freeradbiomed.2021.11.005

Cited by 22 publications

(15 citation statements)

References 37 publications

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“…CCCP and Rosup were used at 10 μM and 50 μg/mL. They are the reported concentrations in the literature and kit instruction [ 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 ]. Only CCCP and Rosup promoted iSS ( Figure 7 A).…”

Section: Resultsmentioning

confidence: 99%

“…When O 2 •− and HO• oxidize Fe-S clusters and release Fe 2+ [ 54 , 55 , 56 ], the sulfur in the cluster is theoretically oxidized to sulfane sulfur (zero valences), as sulfide reacts with ROS to produce sulfane sulfur [ 57 ]. H 2 O 2 and tBH were unable to increase iSS ( Figure 7 A), perhaps partly because they are rapidly metabolized by E. coli cells [ 58 ], partly because they react with sulfane sulfur at a relatively slow rate [ 40 ], and partly because they do not directly damage Fe-S clusters [ 59 ].…”

Section: Discussionmentioning

confidence: 99%

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Optimization of a Method for Detecting Intracellular Sulfane Sulfur Levels and Evaluation of Reagents That Affect the Levels in Escherichia coli

Ran

Yang

et al. 2022

Antioxidants

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Sulfane sulfur is a class of compounds containing zero-valent sulfur. Most sulfane sulfur compounds are reactive and play important signaling roles. Key enzymes involved in the production and metabolism of sulfane sulfur have been characterized; however, little is known about how to change intracellular sulfane sulfur (iSS) levels. To accurately measure iSS, we optimized a previously reported method, in which reactive iSS reacts with sulfite to produce thiosulfate, a stable sulfane sulfur compound, before detection. With the improved method, several factors were tested to influence iSS in Escherichia coli. Temperature, pH, and osmotic pressure showed little effect. At commonly used concentrations, most tested oxidants, including hydrogen peroxide, tert-butyl hydroperoxide, hypochlorous acid, and diamide, did not affect iSS, but carbonyl cyanide m-chlorophenyl hydrazone increased iSS. For reductants, 10 mM dithiothreitol significantly decreased iSS, but tris(2-carboxyethyl)phosphine did not. Among different sulfur-bearing compounds, NaHS, cysteine, S2O32− and diallyl disulfide increased iSS, of which only S2O32− did not inhibit E. coli growth at 10 mM or less. Thus, with the improved method, we have identified reagents that may be used to change iSS in E. coli and other organisms, providing tools to further study the physiological functions of iSS.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Optimization of a Method for Detecting Intracellular Sulfane Sulfur Levels and Evaluation of Reagents That Affect the Levels in Escherichia coli

Ran

Yang

et al. 2022

Antioxidants

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“…The activity of the enzyme was restored by thioredoxin 2 (TRX2) in a concentration- and time-dependent manner [ 89 ]. The TST detoxification function from intra-mitochondrial oxygen free radicals seems also to be protective against persistent oxidative stress, including that induced by radiation, which leads to TST induction [ 90 , 91 , 92 , 93 ]. TST, in association with MST, can promote an anti-oxidative stress function by regulation of sulfane sulfur, GSH or thioredoxin leading to the inhibition of the persistent oxidative stress [ 94 ].…”

Section: Structure and Function Of Tstmentioning

confidence: 99%

Thiosulfate-Cyanide Sulfurtransferase a Mitochondrial Essential Enzyme: From Cell Metabolism to the Biotechnological Applications

Buonvino

Arciero

Melino

2022

IJMS

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Thiosulfate: cyanide sulfurtransferase (TST), also named rhodanese, is an enzyme widely distributed in both prokaryotes and eukaryotes, where it plays a relevant role in mitochondrial function. TST enzyme is involved in several biochemical processes such as: cyanide detoxification, the transport of sulfur and selenium in biologically available forms, the restoration of iron–sulfur clusters, redox system maintenance and the mitochondrial import of 5S rRNA. Recently, the relevance of TST in metabolic diseases, such as diabetes, has been highlighted, opening the way for research on important aspects of sulfur metabolism in diabetes. This review underlines the structural and functional characteristics of TST, describing the physiological role and biomedical and biotechnological applications of this essential enzyme.

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“…These data collectively provide an indirect antioxidant mechanism through which H 2 S signaling may prevent aging and diseases induced by oxidative stress. Nonetheless, a recent study highlighted that H 2 S can stimulate the Fenton reaction, suggesting that it may be a pro-oxidant instead of being an antioxidant [ 60 ]. Therefore, it is critical to explore alternative modes of action, apart from antioxidant paradigm, through which H 2 S can encourage healthy aging and prevent age-related diseases.…”

Section: Antioxidant Activities: a Conventional Anti-aging Paradigm O...mentioning

confidence: 99%

The Potential Implications of Hydrogen Sulfide in Aging and Age-Related Diseases through the Lens of Mitohormesis

Huynh

Wang

et al. 2022

Antioxidants

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The growing increases in the global life expectancy and the incidence of chronic diseases as a direct consequence have highlighted a demand to develop effective strategies for promoting the health of the aging population. Understanding conserved mechanisms of aging across species is believed helpful for the development of approaches to delay the progression of aging and the onset of age-related diseases. Mitochondrial hormesis (or mitohormesis), which can be defined as an evolutionary-based adaptive response to low-level stress, is emerging as a promising paradigm in the field of anti-aging. Depending on the severity of the perceived stress, there are varying levels of hormetic response existing in the mitochondria called mitochondrial stress response. Hydrogen sulfide (H2S) is a volatile, flammable, and toxic gas, with a characteristic odor of rotten eggs. However, H2S is now recognized an important gaseous signaling molecule to both physiology and pathophysiology in biological systems. Recent studies that elucidate the importance of H2S as a therapeutic molecule has suggested its protective effects beyond the traditional understanding of its antioxidant properties. H2S can also be crucial for the activation of mitochondrial stress response, postulating a potential mechanism for combating aging and age-related diseases. Therefore, this review focuses on highlighting the involvement of H2S and its sulfur-containing derivatives in the induction of mitochondrial stress response, suggesting a novel possibility of mitohormesis through which this gaseous signaling molecule may promote the healthspan and lifespan of an organism.

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Rhodanese Rdl2 produces reactive sulfur species to protect mitochondria from reactive oxygen species

Cited by 22 publications

References 37 publications

Optimization of a Method for Detecting Intracellular Sulfane Sulfur Levels and Evaluation of Reagents That Affect the Levels in Escherichia coli

Optimization of a Method for Detecting Intracellular Sulfane Sulfur Levels and Evaluation of Reagents That Affect the Levels in Escherichia coli

Thiosulfate-Cyanide Sulfurtransferase a Mitochondrial Essential Enzyme: From Cell Metabolism to the Biotechnological Applications

The Potential Implications of Hydrogen Sulfide in Aging and Age-Related Diseases through the Lens of Mitohormesis

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