Thiosulfate-Cyanide Sulfurtransferase a Mitochondrial Essential Enzyme: From Cell Metabolism to the Biotechnological Applications

Buonvino, Silvia; Arciero, Ilaria; Melino, Sonia

doi:10.3390/ijms23158452

Cited by 13 publications

(15 citation statements)

References 183 publications

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“…Thiosulfate produced by these cells either enters the colon venous blood [26,27], or the lumen of the colon. The key enzyme in this tentative pathway is probably rhodanese [28]. Huisingh and colleagues [15] also found that supplementing the sheep with molybdate and sulfur exclusively in the form of sulfate, did not change the ruminal SRB level and there was over a two-fold decrease in hydrogen sulfi de from the SRB cultures.…”

Section: Methionine Dissimilating Bacteria (Mdb) Degrades Methioninementioning

confidence: 97%

“…Consequently, intestinal epithelia can accept sulfi des as an energy source and sulfi des have been shown to stimulate the proliferation of these cells [38]. In addition to this, the single layer of epithelia functions as a physical barrier preventing sulfi des in the lumen of the intestine, from entering other tissues throughout the body [26,28,39]. Other effects of colonic sulfi des may include the stabilization of the microbiome-mucosa interface in an animal model of an intestinal dysbiosis, the positive infl uence on hydrogen sulfi de homeostasis in mammalian cells and the promotion of infl ammation in the colon in ulcerative colitis (UC) [39][40][41][42].…”

Section: Possible Health Effects Of Luminal Sulfi Des In the Human Colonmentioning

confidence: 99%

“…It is thought that SRB metabolize sulfate from dextran sulfate sodium to hydrogen sulfi de, in this model. Furthermore, the consumption of foods high in sulfur is associated with an increased likelihood of relapse in UC sufferers in remission [28]. Finally, there can be a reduction in the expression of rhodanese messenger RNA and lower rhodanese enzymatic activity in UC.…”

Section: Colonic Luminal Sulfi Des May Promote Infl Ammation In Ucmentioning

confidence: 99%

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Dietary molybdenum may stimulate the growth of colonic sulfur reducing bacteria, increasing hydrogen sulfide levels in the human colon and the possible health effects of an excess of colonic sulfides

James¹

2022

Arch Clin Gastroenterol

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Molybdenum is a trace mineral needed in small quantities by most life forms. In living organisms, a molybdenum atom is found within molybdenum-dependent enzymes or molybdoenzymes. Molybdoenzymes catalyze reactions in carbon, sulfur, and nitrogen metabolism. Only four molbdoenzymes have been identified in humans. Most of the known molybdoenzymes are found in bacteria. Dietary molybdenum can be administrated to humans, to treat Wilson disease and tungsten poisoning; and it may be useful in arthritis. Sulfur-reducing bacteria are the bacterial group that reduces certain sulfur molecules to hydrogen sulfide. These bacteria can inhabit anaerobic parts of the gastrointestinal tract of mammals and are the predominant producer of hydrogen sulfide in the human colon. Hydrogen sulfide plays a major role in the malodor of human flatus. Some individuals have reported an increase in foul odoriferous gases from the colon after molybdenum supplementation. The underlying mechanism as to how this occurs is currently not known. Possible bacteria that are involved could be sulfur-reducing bacteria and methionine dissimilating bacteria. Supplementing sheep with molybdenum and with sulfur exclusively in the form of methionine can stimulate the growth of sulfur-reducing bacteria and increase the level of sulfides in the rumen. The molybdoenzyme, thiosulfate reductase, is found in sulfur-reducing bacteria and catalyzes the reduction of thiosulfate to hydrogen sulfide. The source of thiosulfate could be from ruminal epithelial cells detoxifying methanethiol, produced by methionine dissimilating bacteria, degrading the dietary methionine to methanethiol. Therefore, the molybdenum could be activating thiosulfate reductases of sulfur-reducing bacteria in the rumen of these animals. The human colon can also harbor sulfur-reducing bacteria, and dietary molybdenum and methionine can reach this organ. Therefore, dietary molybdenum may be stimulating the growth of sulfur-reducing bacteria in some individuals. Sulfides in the human colon could have beneficial and detrimental effects on health. Such effects could include the already mentioned malodor of flatus, the stabilizing of the microbiome-mucosa interface in an intestinal dysbiosis, the treatment of hypertension and the promotion of inflammation in ulcerative colitis.

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Section: Methionine Dissimilating Bacteria (Mdb) Degrades Methioninementioning

confidence: 97%

Section: Possible Health Effects Of Luminal Sulfi Des In the Human Colonmentioning

confidence: 99%

Section: Colonic Luminal Sulfi Des May Promote Infl Ammation In Ucmentioning

confidence: 99%

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Dietary molybdenum may stimulate the growth of colonic sulfur reducing bacteria, increasing hydrogen sulfide levels in the human colon and the possible health effects of an excess of colonic sulfides

James¹

2022

Arch Clin Gastroenterol

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“…(Clarke, 2010), while cyanogenic glycosides are abundant in cassava, stone fruits, and almonds, among other food plants (Jones, 1998; Nyirenda, 2020). Cyanide is detoxified into SCN − by the mitochondrial enzyme rhodanese, largely in the liver (Buonvino et al, 2022). The opportunistic pathogen Pseudomonas aeruginosa , like some other bacteria, produces significant amounts of cyanide (Letoffe et al, 2022), which may increase SCN − concentrations in P. aeruginosa ‐infected individuals.…”

Section: Introduction To Hypothiocyanitementioning

confidence: 99%

Hypothiocyanite and host–microbe interactions

Meredith

Gray

2023

Molecular Microbiology

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The pseudohypohalous acid hypothiocyanite/hypothiocyanous acid (OSCN − /HOSCN) has been known to play an antimicrobial role in mammalian immunity for decades. It is a potent oxidant that kills bacteria but is non-toxic to human cells. Produced from thiocyanate (SCN − ) and hydrogen peroxide (H 2 O 2 ) in a variety of body sites by peroxidase enzymes, HOSCN has been explored as an agent of food preservation, pathogen killing, and even improved toothpaste. However, despite the well-recognized antibacterial role HOSCN plays in host-pathogen interactions, little is known about how bacteria sense and respond to this oxidant. In this work, we will summarize what is known and unknown about HOSCN in innate immunity and recent advances in understanding the responses that both pathogenic and non-pathogenic bacteria mount against this antimicrobial agent, highlighting studies done with three model organisms, Escherichia coli, Streptococcus spp., and Pseudomonas aeruginosa.

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“…9 Hence, the major pathway for cyanide metabolism is the conversion of cyanide (CN − ) to thiocyanate (SCN − ), in the presence of a sulphur donor by the enzyme rhodanese (thiosulfate cyanide sulfur transferase; EC 2.8.1.1). 10 Thiocyanate is the primary detoxification metabolite accounting for up to 80 percent of cyanide removal. Rhodanese (thiosulphate: cyanide sulfurtransferase; EC 2.8.1.1).…”

Section: Introductionmentioning

confidence: 99%

Environmental risk assessments of uses cyanide in gold extraction on water quality parameters and its effect on rhodanese activities in selected organs of coptodon zillii in igun reservoir, Southwestern, Nigeria

Okunola¹,

Komolafe²,

Okonji³

et al. 2022

OAJS

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This study was conducted to investigate the effect of cyanide on the pattern of rhodanese activity distribution in selected organs of Coptodon zillii and water quality of Igun reservoir. Fish and water samples were collected monthly for period of a year to cover both seasons. The physical parameters such as air and water temperature, conductivity, TDS, pH, depth, and transparency were determined. Air temperature and water temperature were determined in situ with a mercury-in-glass thermometer, pH and TDS were determined by calibrated pH meter (PCE-PHD Version 1.1 Model Q656697). Selected organs such as gill, fillet, and liver were used for rhodanese activity analysis while physico-chemical parameters were analyzed based on APHA (2012) standard methods. The pH concentration recorded in this study ranged from 7.1 - 8.1 with a mean of 7.48 ± 0.47 while highest pH mean concentration was observed in dry season (7.61 ± 0.5) compared with the rainy season (7.36 ± 0.24). The mean cyanide concentration was high during the rainy season (0.09 ± 0.03 mg/L) compared with dry season (0.07 ± 0.04 mg/L). The results of rhodanese activity recorded in the gills, fillet, and liver of Coptodon zillii (3.66 ± 3.19 RU/mg, 3.73 ± 2.58 RU/mg, and 3.73 ± 2.68 RU/mg) respectively. Water temperature, pH, and depth showed a positive correlation with rhodanese activity in the gills while a negative correlation was observed between water temperature and rhodanese in the fillet and liver of Coptodon zilllii. Based on the cyanide concentration recorded in this study shown that Igun reservoir is polluted which can be can harmful to humans and other organisms could due to archaic gold mining method using by local miner around the reservoir. Coptodon zillii inhabiting the reservoir showed a high concentration of rhodanese in the different tissues that could serve as the detoxifying mechanism. However, further biochemical analysis of others fish tissue should be examined to determine the effects of mining pollutants on aquatic biota.

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Thiosulfate-Cyanide Sulfurtransferase a Mitochondrial Essential Enzyme: From Cell Metabolism to the Biotechnological Applications

Cited by 13 publications

References 183 publications

Dietary molybdenum may stimulate the growth of colonic sulfur reducing bacteria, increasing hydrogen sulfide levels in the human colon and the possible health effects of an excess of colonic sulfides

Dietary molybdenum may stimulate the growth of colonic sulfur reducing bacteria, increasing hydrogen sulfide levels in the human colon and the possible health effects of an excess of colonic sulfides

Hypothiocyanite and host–microbe interactions

Environmental risk assessments of uses cyanide in gold extraction on water quality parameters and its effect on rhodanese activities in selected organs of coptodon zillii in igun reservoir, Southwestern, Nigeria

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