Rho plays a key role in TGF-β1-induced proliferation and cytoskeleton rearrangement of human periodontal ligament cells

Wang, Li; Wang, Tingle; Meng, Shaoping; Pan, Jinsong

doi:10.1016/j.archoralbio.2013.11.004

Cited by 13 publications

(10 citation statements)

References 22 publications

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“…These results suggest that transgelin may be involved in TGF‐β1 signaling for cellular proliferation of HPDLCs, but not in TGF‐β1 signaling for collagen production. A previous report demonstrated that TGF‐β1 stimulates the proliferation of HPDLCs via Rho GTPase‐dependent pathways that modulate ROCK, LIM kinase and cofilin activity, suggesting that cytoskeletal components are critical for TGF‐β1‐induced proliferation of HPDLCs . Because transgelin is a member of the calponin family of actin‐binding proteins and localized in the cytoskeleton, transgelin may also function with Rho GTPase‐dependent pathways to enhance PDL cell proliferation .…”

Section: Discussionmentioning

confidence: 99%

“…One of the functions of TGF-β1 in PDL cell activities is the regulation of cellular proliferation. Although some reports have shown that TGF-β1 treatment induced PDL cell proliferation via Smad2/3 phosphorylation, LIM domain mineralized protein-1 expression mediated by TAK1-JNK/p38 kinase activation, or Rho GTPase-dependent pathways modulating ROCK, LIM kinase and cofilin activity, [13][14][15] the detailed mechanism by which TGF-β1 stimulates PDL cell proliferation has not been clearly demonstrated yet.…”

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confidence: 99%

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Transgelin mediates transforming growth factor‐β1‐induced proliferation of human periodontal ligament cells

Mitarai

Wada

Hasegawa

et al. 2017

J of Periodontal Research

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

Transgelin mediates transforming growth factor‐β1‐induced proliferation of human periodontal ligament cells

Mitarai

Wada

Hasegawa

et al. 2017

J of Periodontal Research

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“…Rho is a notable coordinator of the cytoskeleton [62], it was suggested in a previous study that the small Rho GTPase and its downstream effector Rho kinase (ROCK) regulate TGFB1-induced remodelling of mammary epithelial cell-to-cell contact [63]. In accordance to the stated, Wang et al showed that TGFB1 can induce proliferation and cytoskeletal rearrangement in periodontal ligament cells via Rho GTPase-dependent pathways [64]. The high abundance of TGFB1 in dogs with dental calculus associated with the presence of Rho GTPase pathway is a relevant finding that may suggest, for futher investigations, TGFB1 as a biomarker candidate of periodontal disease in this species.…”

Section: Discussionmentioning

confidence: 83%

Salivary proteomic profile of dogs with and without dental calculus

et al. 2020

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Background: Dogs' saliva is a complex mixture of inorganic and organic constituents, rich in proteins. Therefore, knowing the saliva composition of these animals is extremely important to identify the presence of proteins that may be involved in physiological and pathological mechanisms of their oral cavity. The present study aimed to characterize the proteomic profile of saliva from dogs with and without dental calculus. Results: Saliva samples were collected from 20 dogs. Before the collection, a visual clinical examination was performed and 8 subjects (40%) did not present any signs of dental calculus, while 12 (60%) presented dental calculus. After saliva collection, the samples were submitted to protein quantification (mBCA), and then they were prepared for analysis by nLC-ESI-MS/MS. A total of 658 unique proteins were identified, of which 225 were specific to dogs without dental calculus, 300 were specific to dogs with dental calculus, and 133 were common to all subjects. These proteins presented functions including transportation, immune response, structural, enzymatic regulation, signal transduction, transcription, metabolism, and some proteins perform functions as yet unknown. Several salivary proteins in dogs with dental calculus differed from those found in the group without dental calculus. Among the abundant proteins detected in periodontal affected cases, can be highlighting calcium-sensing receptor and transforming growth factor beta. Enrichment analysis reveled the presence of Rho GTPases signaling pathway. Conclusions: This research identified salivary proteins, that should be further investigated as potencial biomarkers of chronic periodontits with dental calculus formation in dogs.

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“…Recent decades, lots of publications focused on the study of SC proliferation (Atanasoski et al, 2004 ; Deng et al, 2017 ; Pan et al, 2017 ; Piñero et al, 2017 ). As molecular switches that control the organization and dynamics of the actin cytoskeleton, Rho GTPases are considered to be key regulators of proliferation of various cells (Hu et al, 2014 ; Wang et al, 2014 ). Our previous study indicated that lentivirus-mediated RhoA knocking down to reduce the total protein level of RhoA could significantly slack the proliferation of SCs (Wen et al, 2017a ).…”

Section: Discussionmentioning

confidence: 99%

Inhibition of RhoA-Subfamily GTPases Suppresses Schwann Cell Proliferation Through Regulating AKT Pathway Rather Than ROCK Pathway

Tan

Wen

et al. 2018

Front. Cell. Neurosci.

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Inhibiting RhoA-subfamily GTPases by C3 transferase is widely recognized as a prospective strategy to enhance axonal regeneration. When C3 transferase is administered for treating the injured peripheral nerves, Schwann cells (SCs, important glial cells in peripheral nerve) are inevitably impacted and therefore SC bioeffects on nerve regeneration might be influenced. However, the potential role of C3 transferase on SCs remains elusive. Assessed by cell counting, EdU and water-soluble tetrazolium salt-1 (WST-1) assays as well as western blotting with PCNA antibody, herein we first found that CT04 (a cell permeable C3 transferase) treatment could significantly suppress SC proliferation. Unexpectedly, using Y27632 to inhibit ROCK (the well-accepted downstream signal molecule of RhoA subfamily) did not impact SC proliferation. Further studies indicated that CT04 could inactivate AKT pathway by altering the expression levels of phosphorylated AKT (p-AKT), PI3K and PTEN, while activating AKT pathway by IGF-1 or SC79 could reverse the inhibitory effect of CT04 on SC proliferation. Based on present data, we concluded that inhibition of RhoA-subfamily GTPases could suppress SC proliferation, and this effect is independent of conventional ROCK pathway but involves inactivation of AKT pathway.

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Rho plays a key role in TGF-β1-induced proliferation and cytoskeleton rearrangement of human periodontal ligament cells

Cited by 13 publications

References 22 publications

Transgelin mediates transforming growth factor‐β1‐induced proliferation of human periodontal ligament cells

Transgelin mediates transforming growth factor‐β1‐induced proliferation of human periodontal ligament cells

Salivary proteomic profile of dogs with and without dental calculus

Inhibition of RhoA-Subfamily GTPases Suppresses Schwann Cell Proliferation Through Regulating AKT Pathway Rather Than ROCK Pathway

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