Transgelin mediates <scp>transforming growth factor</scp>‐β1‐induced proliferation of human periodontal ligament cells

Mitarai, Hiromi; Wada, Naohisa; Hasegawa, Daigaku; Yoshida, Shinichiro; Sonoda, Miki; Tomokiyo, Atsushi; Serita, S.; Mizumachi, Hiroyuki; Maeda, Hidefumi

doi:10.1111/jre.12466

Cited by 9 publications

(7 citation statements)

References 41 publications

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“…Notable among these DE lncRNAs were transgelin up‐regulation and neurofilament light chain protein down‐regulation in peri‐implantitis. Transgelin is involved in regulating actin structures and is implicated in the proliferation of periodontal ligament cells; however, its role in peri‐implant tissues remains unknown. The notable down‐regulation of a neurofilament‐related lncRNA possibly reflects the absence of periodontal ligament receptors in the peri‐implant tissue.…”

Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Liu

et al. 2019

J of Periodontal Research

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Background and Objective:Peri-implantitis is a biofilm-mediated infectious disease that results in progressive loss of implant-supporting bone. As compared to its analogue periodontitis, peri-implantitis is generally known to be more aggressive, with comparatively rapid progression and less predictable treatment outcomes, especially when advanced. An understanding of molecular mechanisms underpinning the similarities and differences between peri-implantitis and periodontitis is essential to develop novel management strategies. This study aimed to compare long non-coding RNAs (lncRNAs) and messenger RNA (mRNA) expression profiles between peri-implantitis and periodontitis.Methods: Inflamed soft tissue from peri-implantitis and periodontitis lesions, and healthy gingival tissue controls were analyzed by microarray. Cluster graphs, gene ontology (GO) analysis, and pathway analysis were performed. Quantitative real-time PCR was employed to verify microarray results. The expression levels of RANKL and OPG in the three tissue types were also evaluated, using qRT-PCR. Coding non-coding (CNC) network analyses were performed.Results: Microarray analyses revealed 1079 lncRNAs and 1003 mRNAs as differentially expressed in peri-implantitis when compared to periodontitis. The cyclooxygenase-2 pathway was the most up-regulated biological process in peri-implantitis as compared to periodontitis, whereas hemidesmosome assembly was the most down-regulated pathway. Osteoclast differentiation was relatively up-regulated, and RANKL/OPG ratio was higher in peri-implantitis than in periodontitis. Conclusions:The study demonstrated that peri-implantitis and periodontitis exhibit significantly different lncRNA and mRNA expression profiles, suggesting that osteoclast differentiation-related pathways are comparatively more active in periimplantitis. These data highlight potential molecular targets for periodontitis and peri-implantitis therapy development. K E Y W O R D SlncRNA, microarray, peri-implantitis, periodontitis, transcriptome | 343 LIU et aL.

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Section: Discussionmentioning

confidence: 99%

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Liu

et al. 2019

J of Periodontal Research

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“…Total RNA was reverse‐transcribed with random 6‐mers and ExScript RTase for 15 minutes at 42°C; the reaction was stopped by incubation for 2 minutes at 99°C, followed by 5 minutes at 5°C. PCR was performed using Platinum Taq DNA polymerase (Invitrogen) in a PCR Thermal Cycler Dice (Takara) under the following conditions: 94°C for 2 minutes, then the appropriate number of cycles at [94°C for 30 seconds; appropriate annealing temperature for 30 seconds; 72°C for 30 seconds], and finally 72°C for 7 minutes, in accordance with our recent study . Primer sequences, annealing temperatures, cycle number, and product sizes for RSPO2 , LGR4 , LGR5 , LGR6 , and glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) are shown in Table .…”

Section: Methodsmentioning

confidence: 99%

R‐spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β‐catenin signaling pathway

Arima

Hasegawa

Yoshida

et al. 2018

J of Periodontal Research

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Objective: In this study, we measured the expression of R-spondin 2 (RSPO2) in periodontal ligament (PDL) tissue and cells. Further, we examined the effects of RSPO2 on osteoblastic differentiation of immature human PDL cells (HPDLCs). Background: R-spondin (RSPO) family proteins are secreted glycoproteins that play important roles in embryonic development and tissue homeostasis through activation of the Wnt/β-catenin signaling pathway. RSPO2, a member of the RSPO family, has been reported to enhance osteogenesis in mice. However, little is known regarding the roles of RSPO2 in PDL tissues. Methods: Expression of RSPO2 in rat PDL tissue and primary HPDLCs was examined by immunohistochemical and immunofluorescence staining, as well as by semiquantitative RT-PCR. The effects of stretch loading on the expression of RSPO2 and Dickkopf-related protein 1 (DKK1) were assessed by quantitative RT-PCR. Expression of receptors for RSPOs, such as Leucine-rich repeat-containing G-protein-coupled receptors (LGRs) 4, 5, and 6 in immature human PDL cells (cell line 2-14, or 2-14 cells), was investigated by semiquantitative RT-PCR. Mineralized nodule formation in 2-14 cells treated with RSPO2 under osteoblastic inductive condition was examined by Alizarin Red S and von Kossa stainings. Nuclear translocation of β-catenin and expression of active β-catenin in 2-14 cells treated with RSPO2 were assessed by immunofluorescence staining and Western blotting analysis, respectively. In addition, the effect of Dickkopf-related protein 1 (DKK1), an inhibitor of Wnt/β-catenin signaling, was also examined. Results: Rat PDL tissue and HPDLCs expressed RSPO2, and HPDLCs also expressed RSPO2, while little was found in 2-14 cells. Expression of RSPO2 as well as DKK1 inHPDLCs was significantly upregulated by exposure to stretch loading. LGR4 was predominantly expressed in 2-14 cells, which expressed low levels of LGR5 and LGR6. RSPO2 enhanced the Alizarin Red S and von Kossa-positive reactions in 2-14 cells. In addition, DKK1 suppressed nuclear translocation of β-catenin, activation of βcatenin, and increases of Alizarin Red S and von Kossa-positive reactions in 2-14 cells, all of which were induced by RSPO2 treatment.

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“…To investigate the function of β2‐AR in cells derived from PDL tissue, an expression vector coding human β2‐AR was transfected into 2 to 23 cells, which strongly express PDL‐related molecules 28 . The 2 to 23 cells transfected with a β2‐AR vector showed higher expression of β2‐AR than those cells transfected with empty vector in the gene (Figure 2A) and protein (Figure 2B) levels.…”

Section: Resultsmentioning

confidence: 99%

“…To investigate the function of β2-AR in cells derived from PDL tissue, an expression vector coding human β2-AR was transfected into 2 to 23 cells, which strongly express PDLrelated molecules. 28 The 2 to 23 cells transfected with a β2-AR vector showed higher expression of β2-AR than those cells transfected with empty vector in the gene (Figure 2A) and protein ( Figure 2B) levels. The gene expression of PDLrelated genes-α-SMA and COL1-significantly increased in 2 to 23 cells transfected with a β2-AR vector, compared with those cells transfected with an empty vector ( Figure 2C).…”

Section: Expression Of Pdl-related Molecules In 2 To 23 Transfectedmentioning

confidence: 86%

Functions of beta2‐adrenergic receptor in human periodontal ligament cells

Tomokiyo

Hasegawa

Yuda

et al. 2020

J of Cellular Biochemistry

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Adrenergic receptors (ARs) are receptors of noradrenalin and adrenalin, of which there are nine different subtypes. In particular, β2 adrenergic receptor (β2-AR) is known to be related to the restoration and maintenance of homeostasis in bone and cardiac tissues; however, the functional role of signaling through β2-AR in periodontal ligament (PDL) tissue has not been fully examined. In this report, we investigated that β2-AR expression in PDL tissues and their features in PDL cells. β2-AR expressed in rat PDL tissues and human PDL cells (HPDLCs) derived from two different patients (HPDLCs-2G and-3S). Rat PDL tissue with occlusal loading showed high β2-AR expression, while its expression was downregulated in that without loading. In HPDLCs, β2-AR expression was increased exposed to stretch loading. The gene expression of PDL-related molecules was investigated in PDL clone cells (2-23 cells) overexpressing β2-AR. Their gene expression and intracellular cyclic adenosine monophosphate (cAMP) levels were also investigated in HPDLCs treated with a specific β2-AR agonist, fenoterol (FEN). Overexpression of β2-AR significantly promoted the gene expression of PDL-related molecules in 2 to 23 cells. FEN led to an upregulation in the expression of PDL-related molecules and increased intracellular cAMP levels in HPDLCs. In both HPDLCs, inhibition of cAMP signaling by using protein kinase A inhibitor suppressed the FEN-induced gene expression of α-smooth muscle actin. Our findings suggest that the occlusal force is important for β2-AR expression in PDL tissue and β2-AR is involved in fibroblastic differentiation and collagen synthesis of PDL cells. The signaling through β2-AR might be important for restoration and homeostasis of PDL tissue.

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Transgelin mediates transforming growth factor‐β1‐induced proliferation of human periodontal ligament cells

Abstract: Transgelin is expressed in PDL tissue and might have a role in HPDLC proliferation induced by TGF-β1 stimulation.

Cited by 9 publications

References 41 publications

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

Long non‐coding RNA and mRNA expression profiles in peri‐implantitis vs periodontitis

R‐spondin 2 promotes osteoblastic differentiation of immature human periodontal ligament cells through the Wnt/β‐catenin signaling pathway

Functions of beta2‐adrenergic receptor in human periodontal ligament cells

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