Rho-Kinase Activation in Patients With Heart Failure

Do.e, Zhulanqiqige; Fukumoto, Yoshihiro; Sugimura, Koichiro; Miura, Yutaka; Tatebe, Shunsuke; Yamamoto, Shozo; Aoki, Tatsuo; Nochioka, Kotaro; Nergui, Suvd; Yaoita, Nobuhiro; Satoh, Kimio; Kondo, Masateru; Nakano, Masataka; Wakayama, Yuji; Fukuda, Koji; Nihei, Takayuki; Kikuchi, Yoku; Takahashi, Jun; Shimokawa, Hiroaki

doi:10.1253/circj.cj-13-0397

Cited by 35 publications

(33 citation statements)

References 49 publications

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“…Compared with healthy individuals, leukocyte ROCK activity is increased in patients with stable chronic congestive heart failure, as well as in individuals with acute heart failure (Ocaranza et al, 2011;Dong et al, 2012;Do e et al, 2013). Upregulation of ROCK1, ROCK2, and RhoA expression was also detected in leukocytes from patients with congestive heart failure compared with controls (Dong et al, 2012).…”

Section: A Cardiovascular Diseasesmentioning

confidence: 95%

“…Upregulation of ROCK1, ROCK2, and RhoA expression was also detected in leukocytes from patients with congestive heart failure compared with controls (Dong et al, 2012). Whether increased ROCK activity is a marker of the deterioration of systolic function is still unclear, because reports have suggested both that a correlation with left ventricular ejection fraction exists (Ocaranza et al, 2011;Dong et al, 2012) and that there is no correlation (Do e et al, 2013). Nevertheless, the combination of ROCK activity and N-terminal prohormone of brain natriuretic peptide (NT-proBNP) was found to be more useful for predicting mortality in patients with chronic heart failure than NT-proBNP alone.…”

Section: A Cardiovascular Diseasesmentioning

confidence: 99%

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Rho Kinases in Health and Disease: From Basic Science to Translational Research

Loirand¹

2015

Pharmacol Rev

161

133

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Section: A Cardiovascular Diseasesmentioning

confidence: 95%

Section: A Cardiovascular Diseasesmentioning

confidence: 99%

Rho Kinases in Health and Disease: From Basic Science to Translational Research

Loirand¹

2015

Pharmacol Rev

161

133

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“…Over the past decade, there has been increasing experimental evidence underlining the involvement of two isoforms of ROCK, ROCK1 and ROCK2 in numerous cardiovascular and renal disorders. The activity of this pathway is increased in disorders such as heart failure, coronary artery disease and cerebrovascular disease, all of which associated with tissue hypoxia and reduced integrity of tissue vasculature (18,19,23).…”

Section: Discussionmentioning

confidence: 99%

“…brain, heart etc.) (18,19). In a single study, ROCK activity has been shown to be increased in OSAS in correlation with increased C-reactive protein (CRP) concentration and reduced flow-mediated dilatation in the brachial artery suggesting the presence of an interplay with ROCK and endothelial dysfunction (20).…”

mentioning

confidence: 99%

Reduced Peripheral Blood Mononuclear Cell ROCK1 and ROCK2 Levels in Obstructive Sleep Apnea Syndrome

2018

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Abstract. Background Obstructive sleep apnea syndrome (OSAS) is one of the most frequent sleep disorders with a prevalence of 2-4% in the adult population, 3.1%-7.5% in men and 1.8%-5% in women (1-9). OSAS is characterized by repetitive episodes of apnea due to total compression of respiratory tract, intermittent hypoxia, reduced blood oxygen saturation and impaired sleep quality. It may cause difficulty sleeping, morning headache, daytime sleepiness, fatigue and reduced performance in daytime activities. Age, male gender and obesity have been listed among the risk factors of OSAS (1-3, 10). Moreover, OSAS has been associated with increased risk for arterial and pulmonary hypertension, cardiovascular and cerebrovascular disorders and metabolic syndrome (2,(11)(12)(13)(14).Although pathophysiological processes underlying OSAS have not been fully understood, inflammation, vascular endothelial dysfunction and altered body metabolism have been implicated (15,16). Rho-associated protein kinase (ROCK) enzyme is known to be involved in regulation of vascular functions and cardiovascular disease pathogenesis. ROCK activation has been shown to enhance endothelial permeability and induce angiogenic processes through regulation of endothelial gene expression (17). In several studies, ROCK enzyme activity has been shown to be altered in patients characterized with cardiorespiratory problems that might potentially lead to chronic hypoxia of the target tissue (e.g. brain, heart etc.) (18,19). In a single study, ROCK activity has been shown to be increased in OSAS in correlation with increased C-reactive protein (CRP) concentration and reduced flow-mediated dilatation in the brachial artery suggesting the presence of an interplay with ROCK and endothelial dysfunction (20).The present study was conducted to find out whether intermittent hypoxia induced by OSAS might alter ROCK1 and ROCK2 expression in the peripheral blood and thus levels of these proteins can be used to extrapolate the intensity of hypoxia induced by OSAS. Clinical and laboratory features that might potentially be associated with ROCK expression levels were also investigated. 319This article is freely accessible online.Correspondence to: Erdem Tüzün,

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“…Circulating leukocytes were isolated from venous blood samples as previously reported. 15, 17 To measure Rho-kinase activity, Western blot analysis was performed by an independent physician who was blinded to the clinical patient information as previously described. 15,17,18 NIH 3T3 cell lysates were used as a positive control and to standardize the results of Western blot analysis from several membranes.…”

Section: Nihei T Et Almentioning

confidence: 99%