Rhizospheric rhizobia identification in maize (Zea mays L.) plants

Abstract

Rhizobia have been studied for the symbiosis that they establish with the roots of legumes. However, the colonization and promotion of growth in non-leguminous plants has also been demonstrated. The aim of this work was the biochemical and molecular identification of rhizosphere rhizobia present inthe rhizosphere of two commercial maize cultivars. Cultivableisolates were obtained in yeast-mannitol-agar (YMA) mediumfrom rhizospheric soil and the rhizoplane. The cultural (size,color, mucus, etc.), morphological, and staining (cell shape,response to staining and sporulation) characteristics weredetermined as well as isolate responses to eight biochemicaltests (acid-base production, citrate, oxidase, catalase, H2Sproduction, urease, gelatinase and the oxidative-fermentativeassay) that are valuable for rhizobia identification. The genuswas determined by 16S rDNA gene sequencing. We obtained 81total isolates of which 30.86% showed the cultural, morphological and staining characteristics expected for rhizobia and only 20% of these corresponded to the genus Rhizobium.

Rhizobium Grants the Reduction of Phosphate Fertilization during the Production of Coffee Seedlings

“…Rhizobia are bacteria that have been traditionally studied for their ability to establish a symbiotic relationship with leguminous plants and to induce nodule formation where they fix nitrogen (N) [36]. The use of these bacteria in some studies has revealed their beneficial effects on nonleguminous plants such as corn (Zea mays L.) [37] and rice (Oryza sativa L.) [38]. However, few studies have addressed the role of rhizobia in coffee nutrition.…”

Inoculación de rizobios favorecen el crecimiento de plántulas de Coffea arabica L. injertadas en vivero

“…Las cepas empleadas fueron seleccionadas en estudios previos y se comprobó el efecto estimulador del crecimiento vegetal de las cepas Rhizobium sp. Rpd16, Rpr2, Rpr11 aisladas de la rizósfera de arroz (Hernández y Nápoles, 2017; y Rhizobium sp C1 y C19-2 procedentes del cultivo de maíz (Pérez et al, 2019).…”

“…Por el contrario, la cepa C1 presentó valores similares al testigo fertilizado. Esto último pudiera ser explicado por su principal atributo de oxidasa y gelatinasa positivo (Pérez et al, 2019), esta característica en ella la hace diferente a las otras cepas. Aunque no se cuenta con literatura abundante sobre el efecto de los rizobios en esta variable en cafet.…”

“…Los rizobios son una de estas bacterias que tienen la capacidad de colonizar activamente el sistema radicular para favorecer su crecimiento y rendimiento de plantas no leguminosas (Pérez et al, 2019). Bacterias del género Rizobios han sido empleadas para estimular el desarrollo de diversas especies de plantas como Moringa oleifera Lam (Mazher et al, 2014), Theobroma cacao (Suprano et al, 2015), Oryza sativa L. (Hernández y Nápoles, 2017), Arachis hypogaea L. (Priya et al, 2019), Zea mays L. (Pérez et al, 2019), Vigna unguiculata L. Walp. (Hara et al, 2021), entre otros.…”