Rhizobium sp.CCNWYC119: a single strain highly effective as biofertilizer for three different peas (Pigeon pea, Sweet pea and Chick pea)

Abstract

Rhizobium spp. was isolated from root nodules of Pigeon pea (Cajanus cajan L.), Sweet pea (Lathyrus sativus L.), Chick pea (Cicer arietinum L.). The isolates ware rod shaped, aerobic, gram negative, motile and non-spore forming. Isolates were positive to Catalase, Citrate utilization, Urea hydrolysis, Congored, Nitrification, Oxidase, Triple sugar iron and MacConkey agar test. The isolates can ferment all nine sugars. Then, the isolates identified as Rhizobium spp. depending on above results were subjected to 16S rRNA sequencing for further confirmation and identification. Surprisingly, the isolates were same strain or member of same cluster of Rhizobium and identified as Rhizobium sp.CCNWYC119 strain based on 16S rRNA sequence (98% similarity). Then, different parameters of soil quality enrichment and plant growth viz. plant height; weight of pods and seeds; number, fresh and dry weight of nodules were studied to test the efficacy of the isolate as biofertilizer. Here, inoculant of Rhizobium sp. isolated from Pigeon pea was used as biofertilizer. The results showed the significant increase of nodulation, enrichment of soil of rhizosphere, plant growth and yield for all three types of inoculated peas as compared with non-inoculated control peas indicating that the isolated strain could be used as a common efficient biofertilizer for Pigeon pea, Sweet pea and Chick pea. It was also found that the isolate grew optimally at temperature 28°C and pH 7.0. Moreover, the isolate was sensitive to the higher concentration of NaCl (>1%) and to antibiotics- Mecillinam, Ciprofloxacin, Cotrimoxazole, Pefloxacin, Ceftazidime and Tetracycline.

Effect of urea, bio-fertilizers and their interaction on the growth, yield and yield attributes of Cyamopsis Tetragonoloba

“…(2005) reported that plant height, number of branches, plant fresh and dry weights, umbels number and fruits yield increased with nitrogen and bio-fertilizer treatment in Anethum graveolens, with the high dose of nitrogen producing the highest values. Nushair et al, (2018) also reported in Pigeon pea, Sweet pea and Chick pea that bio fertilizer application significantly increased plant height pod and seed weight. Hassan et al, (2012) stated that biofertilizer treatments enhanced plant height, branch number/ plant and plant dry weight.…”

BamFx Induced Resistance by Seed Priming Method against Fusarium sp. in Cajanus cajan (L.) Mill sp.

“…In other report, Rhizobium spp. was isolated from root nodules of pigeon pea (Nushair et al, 2018). Previous reports on the toxinogenic potential of these fungi involved peanut cakes (S. Adjou, 2012), as well as peanut seeds gathered from Benin (Boli et al, 2020).…”

Salt Tolerance Capacity of Bacteria Isolated From Irrigated Soil in Kano, Nigeria

“…However, there is possibility of bacteria potential for bioremediation of salt from plant rhizosphere. Hence this study was undertaken for the isolation and molecular characterization of the halophilic bacteria from salt affected soils (Nushair et al, 2018). Furthermore, the soil bacteria play a very vital role in biogeochemical cycles of require nutrients resulting in better crop yield.…”