Aim: In this study, we aimed to reveal the relationship between disease activity level and serum 25 (OH) vitamin D level in rheumatoid arthritis (RA), a chronic autoimmune disease. Material and Method: Ninety-one patients with RA and 90 healthy controls were included in the study. DAS28 score and 25 (OH) vitamin D levels of the patient group and control group were compared. Results: The mean serum 25 (OH) vitamin D level of patients and controls were 16,8 ± 10,1 μg / L and 28.3 ± 16 μg / L, respectively. 25 (OH) vitamin D levels were statistically significantly lower in the patients group than in the control group (p <0.001). In total, serum 25 (OH) vitamin D levels and DAS28 scores of the patients were not significantly correlated (r =-0.058, p = 0.588). Correlation analysis according to sexes showed negative correlation between serum 25 (OH) vitamin D level and DAS28 score in males (r =-0,646, p = 0,002) and no correlation was found in females (r = 0,113, p = 0,346). Discussion: Low serum 25 (OH) vitamin D may play a role in the pathogenesis of RA patients. The significant relationship between disease activity and serum 25 (OH) vitamin D level in male gender shows that this effect is more prominent in male sex.