Rhesus Monkey Oocyte Maturation and Fertilization in Vitro: Roles of the Menstrual Cycle Phase and of Exogenous Gonadotropins1

Alak, Baha M.; Wolf, Don P.

doi:10.1095/biolreprod51.5.879

Cited by 27 publications

(10 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Growing oocytes were collected from follicles with diameters of less than 2.5 mm [24] that were mechanically dissected out from monkey ovaries. The granulosa cell-enclosed oocytes were subjected to microinjection in TALP/ Hepes medium [25] and cultured in HECM-9 medium [26] for up to two days after injection.…”

Section: Oocyte and Sperm Collection Fertilization And Embryo Culturementioning

confidence: 99%

WEE2 Is an Oocyte-Specific Meiosis Inhibitor in Rhesus Macaque Monkeys1

Hanna

Yao

Patta

et al. 2010

Biology of Reproduction

View full text Add to dashboard Cite

WEE1 homolog 2 (WEE2, also known as WEE1B) is a newly identified member of the WEE kinase family that is conserved from yeast to humans. The aim of the present study was to determine the spatiotemporal expression pattern and the function of WEE2 during oocyte maturation in a nonhuman primate species, the rhesus macaque. Among 11 macaque tissues examined, WEE2 transcript is predominantly expressed in the ovary and only weakly detectable in the testis. Within the ovary, WEE2 mRNA is exclusively localized in the oocyte and appears to accumulate during folliculogenesis, reaching the highest level in preovulatory follicles. Microinjection of a full-length WEE2-GFP (green fluorescent protein) fusion mRNA indicates a specific nuclear localization of WEE2 protein in both growing and fully grown germinal vesicle (GV)-intact oocytes. Taking the long double-stranded RNA-mediated RNA interference approach, we found that down-regulation of WEE2 led to meiotic resumption in a subset of GV oocytes even in the presence of a phosphodiesterase 3 inhibitor. On the other hand, overexpression of WEE2 delays the reentry of oocytes into meiosis in both mice and monkeys. These findings suggest that WEE2 is a conserved oocyte-specific meiosis inhibitor that functions downstream of cAMP in nonhuman primates.

show abstract

Section: Oocyte and Sperm Collection Fertilization And Embryo Culturementioning

confidence: 99%

WEE2 Is an Oocyte-Specific Meiosis Inhibitor in Rhesus Macaque Monkeys1

Hanna

Yao

Patta

et al. 2010

Biology of Reproduction

View full text Add to dashboard Cite

show abstract

“…We used similar culture conditions in a nonhuman primate model to assess the maturation of oocytes from SAFs during the natural menstrual cycle. In rhesus monkeys, the highest yield of healthy oocytes and highest ratio of healthy to degenerating oocytes were obtained from SAFs during the early follicular phase compared to those from late follicular or luteal phase of the menstrual cycle [34]. Moreover, the cohort of antral follicles in ovaries from the early follicular phase (Days 1-5 of menstrual cycle) represents those from which the dominant follicle will eventually be selected [35].…”

Section: Introductionmentioning

confidence: 99%

Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

Peluffo

Barrett

Stouffer

et al. 2010

Biology of Reproduction

View full text Add to dashboard Cite

The stage at which follicle-enclosed cumulus-oocyte complexes achieve developmental competence in primates is unknown. Therefore, studies were designed to characterize the ability of oocytes in small antral follicles present during the menstrual cycle to spontaneously resume meiosis, fertilize, and support early embryo development. Ovaries were removed from adult rhesus monkeys (n = 12) during the early follicular phase (Days 3-4) of spontaneous cycles. Small antral follicles were divided into five groups according to their diameter; group I: <0.5 mm; group II: 0.5-0.99 mm; group III: 1.0-1.49 mm; group IV: 1.5-1.99 mm; and group V: 2.0-2.5 mm. The cumulus-oocyte complex from healthy small antral follicles (devoid of dark oocytes or granulosa cells) were extracted (n = 199) and cultured for 48 h under different conditions: in TALP (tyrode, albumin, lactate, pyruvate) medium alone, SAGE medium alone, or plus gonadotropins. At 48 h, oocyte meiotic status and diameter were measured after treatment of cumulus-oocyte complexes with hyaluronidase. Cumulus-oocyte complexes derived from follicles of 0.5- to 2-mm diameter contain oocytes that typically reinitiate meiosis in the absence or presence of gonadotropins and fertilize via in vitro fertilization or intracytoplasmic sperm injection. Moreover, the inseminated oocytes can reach the morula stage but arrest. Thus, the ability of these oocytes to complete maturation, as monitored from subsequent embryonic development after fertilization, is suboptimal. Further studies on primate IVM of oocytes from SAFs are warranted in order for them to be considered as an additional, novel source of gametes for fertility preservation in cancer patients.

show abstract

“…The accurate classification of the ovarian cycles of nonhuman primates is of importance to a number of disciplines, constituting the cornerstone of a wide variety of studies ranging from evolutionary studies [e.g., Profet, 1993;Strassmann, 1996], physiology [e.g., Gordon et al, 1995], chronobiology [Harter & Erkert, 1993], to in vivo [e.g., Morrell et al, 1997] and in vitro fertilization [e.g., Alak & Wolf, 1994;Wolf et al, 1996].…”

Section: Introductionmentioning

confidence: 99%

The menstrual cycle of the spider monkey (Ateles geoffroyi)

et al. 1998

View full text Add to dashboard Cite

The ovarian cycles of four adult female spider monkeys (Ateles geoffroyi) were followed daily throughout 30 days by means of vaginal swabs and blood samplings. Cytological analyses of the vaginal swabs and radioimmunoassay determination of the daily levels of estradiol‐17β (E2) and progesterone (P4) were done in order to classify the kind of ovarian cycle of this species. Our results show that Ateles geoffroyi females display menstrual cycles of about 24 days on average. By comparison with the well‐known menstrual cycles of women, apes, and Old World monkeys, the four distinctive cytological phases (bleeding, follicular, periovulatory, and luteal) could be recognized; mid‐cycle E2 peaks followed by mid‐luteal increases of the same hormone were present in all four females. P4 levels were higher after the E2 peak, although both hormones were present throughout the cycles. Also, age‐dependent features, hormone profiles, and changes in menstrual phases lengths were detected. Am. J. Primatol. 44:183–195, 1998. © 1998 Wiley‐Liss, Inc.

show abstract

Rhesus Monkey Oocyte Maturation and Fertilization in Vitro: Roles of the Menstrual Cycle Phase and of Exogenous Gonadotropins1

Cited by 27 publications

References 39 publications

WEE2 Is an Oocyte-Specific Meiosis Inhibitor in Rhesus Macaque Monkeys1

WEE2 Is an Oocyte-Specific Meiosis Inhibitor in Rhesus Macaque Monkeys1

Cumulus-Oocyte Complexes from Small Antral Follicles During the Early Follicular Phase of Menstrual Cycles in Rhesus Monkeys Yield Oocytes That Reinitiate Meiosis and Fertilize In Vitro1

The menstrual cycle of the spider monkey (Ateles geoffroyi)

Contact Info

Product

Resources

About