Rheological study of the dynamic process of fibrinolysis

Kawakami, S.; Kaibara, Makoto; Nakayama, Mahito; Isogai, Yukihide; Ikemoto, Suguru; Ea, O'Rear; Lee, Jae Sung

doi:10.1097/00001721-199709000-00005

Cited by 4 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the contrary, in the present measuring system, the coagulation of PFP in the polypropylene tube used does not occur over a period of 2 h, indicating that the polypropylene surface offers an inert surface against blood coagulation [18]. However, the coagulation of whole blood in the polypropylene tube occurred at 10-60 min, being greatly dependent on the individual in question.…”

Section: Discussioncontrasting

confidence: 66%

“…When the intrinsic coagulation pathway is triggered by the activation of factor XII by glass surface or cephalin, or by the activation of factor XI through the activation of platelets, the coagulation of whole blood and platelet-free plasma (PFP) rapidly occurs, and the Ti value is less dependent on individuals [17][18][19]. On the contrary, in the present measuring system, the coagulation of PFP in the polypropylene tube used does not occur over a period of 2 h, indicating that the polypropylene surface offers an inert surface against blood coagulation [18].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of age and hematocrit on the coagulation of blood

Ujiié

Kawasaki

Suzuki³

et al. 2009

J Biorheol

View full text Add to dashboard Cite

To examine the effects of age, hematocrit and the daily variation in hematocrit on coagulation of blood, the time of onset of coagulation (Ti) of whole blood obtained from donors including normal subjects and patients was measured by means of a rheological technique. The Ti value of recalcified blood decreased with an increase in age, but in donors aged 65 years or more (the elderly), the Ti value was almost independent of age. The Ti value for blood obtained from the elderly was significantly lower at lower hematocrit levels, but that for blood obtained from young donors was almost independent of hematocrit. The daily variation in hematocrit in individuals was small (maximum variation: about 4%), and the variation had little effect on the Ti value. However, a slight increase in hematocrit was considered to bring about a significant increase in viscosity at lower shear rates. Therefore, it is suggested that a slight increase in hematocrit under stagnant flow conditions is one of major risk factors for venous thrombogenesis, especially in the elderly.

show abstract

Section: Discussioncontrasting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Influence of age and hematocrit on the coagulation of blood

Ujiié

Kawasaki

Suzuki³

et al. 2009

J Biorheol

View full text Add to dashboard Cite

show abstract

“…Coagulation of PFP with added F-IXee in a polypropylene tube (length 2.5 cm; diameter 0.7 cm) was monitored using the damped oscillation rheometer. It was confirmed that the polypropylene tube offers an inert surface against the initiation of blood coagulation, being similar to that of the vascular model tube [28]. Therefore, in the experiments described below, the polypropylene tube was used as a sample tube of the rheometer.…”

Section: Procoagulant Ability Of F-ix Cleaved By the Enzymementioning

confidence: 73%

Rheological study on coagulation of blood with special reference to the triggering mechanism of venous thrombus formation

Kaibara

2009

J Biorheol

View full text Add to dashboard Cite

A markedly reduced blood flow, an elevation of hematocrit and an increased aggregability of erythrocytes [red blood cells (RBCs)] are risk factors for venous thrombus formation (intravascular blood coagulation). However, these risk factors alone seem to be insufficient to stimulate the coagulation cascade in the absence of a primary triggering mechanism. In this paper, our rheological and biochemical studies on blood coagulation, especially focusing on procoagulant activity of RBCs, are summarized. It is shown that the intrinsic coagulation pathway is triggered by the activation of factor IX (F-IX) by RBCs. The F-IX-activating enzyme in normal human erythrocyte (RBC) membranes was purified, identified and characterized. The activation of F-IX by RBCs was enhanced by a decrease in flow shear rate and an elevation in hematocrit. The procoagulant ability of RBCs and coagulation of blood obtained from individuals with a relatively high level of hypercoagulability were enhanced compared with those for normals. The studies demonstrated a new triggering mechanism for coagulation or thrombus formation that may occur under stagnant flow conditions.

show abstract

Activation of factor IX by erythrocyte membranes causes intrinsic coagulation

Iwata

Kaibara

2002

Blood Coagulation & Fibrinolysis

View full text Add to dashboard Cite

As an extension of our earlier work, procoagulant activity of erythrocyte [red blood cell (RBC)] membrane was examined using biochemical and rheological techniques. Western blot analysis of coagulation factors incubated with erythrocytes (RBCs) showed that only factor IX (FIX) was activated by RBC membranes in the presence of calcium ions. A fluorogenic assay suggested that activated FIX is capable of activating factor X. A preliminary crude extraction of the substance from RBC membranes suggested that a FIX-activating enzyme may be located on the RBC membrane. The initiation of FIX activation by RBCs was enhanced by an elevation in hematocrit. Moreover, the rate of FIX activation by RBCs from normal pregnant women and diabetic patients was much faster than that from normal subjects. In addition, glucose treatment of normal RBCs resulted in the increase in procoagulant activity. It is suggested that FIX activation by RBC membranes may serve as a triggering mechanism for blood coagulation, although further study will be required to clarify the putative FIX activating enzyme on the RBC membrane and to permit more extensive physiological experiments to be performed.

show abstract

Rheological study of the dynamic process of fibrinolysis

Cited by 4 publications

References 0 publications

Influence of age and hematocrit on the coagulation of blood

Influence of age and hematocrit on the coagulation of blood

Rheological study on coagulation of blood with special reference to the triggering mechanism of venous thrombus formation

Activation of factor IX by erythrocyte membranes causes intrinsic coagulation

Contact Info

Product

Resources

About