Rheological characteristics of desialylated erythrocytes in relation to fibrinogen-induced aggregation

Maeda, Nobuji; Imaizumi, Kazunori; Sekiya, Misuzu; Shiga, Tohru

doi:10.1016/0005-2736(84)90261-x

Cited by 38 publications

(30 citation statements)

References 28 publications

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“…2,14,15 There have been few studies on the role of cell age in erythrocyte aggregation. Greater aggregation for aged versus young erythrocytes has been reported in healthy subjects.…”

mentioning

confidence: 99%

Erythrocyte Disaggregation Shear Stress, Sialic Acid, and Cell Aging in Humans

et al. 1998

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Abstract-Erythrocyte aggregation, which plays an important role in the physiological behavior of blood fluidity, was found to be enhanced in hypertension and hypercholesterolemia. While the role of macromolecule bridging force has been widely described, cellular factors related to membrane sialic acid content, which might contribute to the negative charge of cell surface causing the repulsion of erythrocytes, have been less studied. Cell age-dependent changes in membrane sialic acid content (in micromoles per gram of integral membrane protein) were investigated in 24 normotensive and 24 hypertensive matched subjects, each divided into 2 identical subgroups according to a cutoff of 6.2 mmol/L serum cholesterol. A progressive and significant (PϽ0.001) decrease in membrane sialic acid content associated with an increase (PϽ0.001) of disaggregation shear rate threshold (laser reflectometry in the presence of dextran) were observed with increased erythrocyte density (erythrocytes fractionated by density using ultracentrifugation) in both normotensive and hypertensive groups regardless of the cholesterol level. However, disaggregation shear rate threshold was significantly higher and sialic acid content was lower (PϽ0.001) in both hypertensive and normotensive subjects with hypercholesterolemia compared with either normotensive or hypertensive subjects with low cholesterol, respectively. A high membrane sialic acid content variance, beginning in the younger erythrocytes, was due mainly to triglyceride and LDL cholesterol levels (R 2 ϭ0.49 for low, R 2 ϭ0.43 for middle, and R 2 ϭ0.54 for high densities, ie, young, mean, and senescent erythrocytes, respectively). We conclude that an early decrease in erythrocyte sialic acid content may influence the rheological properties of blood by increasing the adhesive energy of erythrocyte aggregates. (Hypertension. 1998;32:324-330.) Key Words: erythrocyte aggregation Ⅲ rheology Ⅲ sialic acid Ⅲ hypertension, essential Ⅲ hypercholesterolemia E rythrocyte aggregation is one of the main determinants influencing blood circulation at low shear rates by increasing blood viscosity and inducing "sludging" in the capillary.1 Aggregation of red blood cells is a reversible process that occurs when the bridging force due to the adsorption of macromolecules onto adjacent cell surfaces exceeds the disaggregation forces caused by electrostatic repulsion, membrane strain, and mechanical shearing. [1][2][3] An increase in erythrocyte aggregation was found to be associated with cardiovascular risk factors such as hypertension, 4 hyperlipoproteinemia, 5,6 and smoking 7 and in clinical situations such as myocardial ischemia, 8 thromboembolic states, 9 and retinal venous occlusion. 10 In general, most studies have focused on the ability of plasma proteins and various polymers to induce erythrocyte aggregation. We showed that in hypertension and hypercholesterolemia the increase in erythrocyte aggregation could be attributed to an increase in the concentration of plasma fibrinogen. 4,6 In addition, in ...

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“…2,14,15 There have been few studies on the role of cell age in erythrocyte aggregation. Greater aggregation for aged versus young erythrocytes has been reported in healthy subjects.…”

mentioning

confidence: 99%

Erythrocyte Disaggregation Shear Stress, Sialic Acid, and Cell Aging in Humans

et al. 1998

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“…Crystal structure analysis confirmed that there is only one site on the HN protein with dual functions, the HA and NA activities (33). By possessing these activities, NDV can cause hemagglutination of red blood cells (35)(36)(37)(38)(39)(40). The addition of ·HN-IL-2 to NDV is shown here to prevent both HA and NA activities simultaneously.…”

Section: Discussionmentioning

confidence: 84%

Selective gene transfer to tumor cells by recombinant Newcastle Disease Virus via a bispecific fusion protein

Bian

Fournier²,

Moormann³

et al. 2005

Int J Oncol

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Abstract. Much interest exists presently in development of vectors for gene therapy of tumors based on RNA viruses because these viruses replicate in the cytoplasm and do not integrate into DNA. The negative stranded paramyxovirus, Newcastle Disease Virus (NDV) from chicken has the additional advantages of preferential replication in tumor cells and of oncolytic and immunostimulatory properties. We here describe the bispecific fusion protein ·HN-IL-2 which binds to NDV, inhibits its normal cell binding property and introduces a new binding specificity for the interleukin-2 receptor (IL-2R). We demonstrate selective gene transfer to tumor cells expressing IL-2R via the bispecific fusion protein when using recombinant NDV carrying as marker gene the enhanced green fluorescence protein (NDFL-EGFP). Hemadsorption (HA) and neuraminidase activities (NA) of the HN protein of NDV were shown to be blocked by ·HN-IL-2 simultaneously and the absence of HA-activity of modified NDV was confirmed in vivo. Retargeted virusbinding to IL-2R positive tumor cells was not sufficient for the process of cellular infection. It required in addition membrane fusion via the viral F-protein. By modification of recombinant NDV with a bispecific molecule, our results demonstrate a novel and safe strategy for selective gene transfer to targeted tumor cells.

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“…(4) Sialic acid on the erythrocyte membrane surface provides electrostatic repulsive force among the cells, and it plays a protective role against the elimination of the cells from the circulation. The reduction of sialic acid in a hereditary disease remarkably enhances erythrocyte aggregation, and the phenomenon is experimentally demonstrated in neuraminidase-treated cells [9]. It must be noted that the negative charge-reduced cells never aggregate without macromolecules [9].…”

Section: Interest In Erythrocyte Aggregationmentioning

confidence: 98%

“…The reduction of sialic acid in a hereditary disease remarkably enhances erythrocyte aggregation, and the phenomenon is experimentally demonstrated in neuraminidase-treated cells [9]. It must be noted that the negative charge-reduced cells never aggregate without macromolecules [9]. (5) Structural changes of membrane proteins of erythrocytes modify the erythrocyte aggregation: typically in vivo aged cells induce more aggregation in spite of the reduced deformability, as compared with young cells [10].…”

Section: Interest In Erythrocyte Aggregationmentioning

confidence: 99%