Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: Gastroprotective and Ulcer Healing Properties in Rats

Maria-Ferreira, Daniele; Silva, Luísa Mota da; Mendes, Dayse; Cabrini, Daniela de Almeida; Nascimento, Adamara Machado; Iacomini, Marcello; Cipriani, Thales R.; Santos, Adair R.S.; Werner, Maria Fernanda de Paula; Baggio, Cristiane Hatsuko

doi:10.1371/journal.pone.0084762

Cited by 45 publications

(29 citation statements)

References 40 publications

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“…However, the adverse effects remain a major concern for prolonged usage, which also may include peptic ulceration and wound healing impairment 18 . In this sense, treatment with RGal not only improved the general parameters of the disease in DSS model but we demonstrated previously that RGal also protected the gastric mucosa against formation of acute lesions and accelerated the healing of chronic ulcers in rats 12 , suggesting that RGal could be better option to the treatment of UC.…”

Section: Discussionmentioning

confidence: 70%

“…Interestingly, our previous studies revealed that this polysaccharide protected the gastric mucosa against acute lesions induced by ethanol and promoted gastric ulcer healing in a chronic ulcer model induced by acetic acid in rats. These effects were due to the increase of cellular proliferation and gastric mucus content, associated with the reduction of inflammatory parameters and oxidative stress, without toxicological effects after sub chronic exposure 12 .…”

Section: Introductionmentioning

confidence: 96%

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Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human Caco-2 cells

Maria-Ferreira

Nascimento

Cipriani

et al. 2018

Sci Rep

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Natural polysaccharides have emerged as an important class of bioactive compounds due their beneficial biological effects. Here we investigated the protective and healing effects of rhamnogalacturonan (RGal) isolated from Acmella oleracea (L.) R.K. Jansen leaves in an experimental model of intestinal inflammation in mice and in heterogeneous human epithelial colorectal adenocarcinoma cells (Caco-2). The findings demonstrated that RGal treatment for 7 days reduced the severity of DSS-induced colitis by protecting mice from weight loss, macroscopic damage and reduction of colon length. When compared to the DSS group, RGal also protected the colon epithelium and promoted the maintenance of mucosal enterocytes and mucus secreting goblet cells, in addition to conserving collagen homeostasis and increasing cell proliferation. In an in vitro barrier function assay, RGal reduced the cellular permeability after exposure to IL-1β, while decreasing IL-8 secretion and claudin-1 expression and preserving the distribution of occludin. Furthermore, we also observed that RGal accelerated the wound healing in Caco-2 epithelial cell line. In conclusion, RGal ameliorates intestinal barrier function in vivo and in vitro and may represent an attractive and promising molecule for the therapeutic management of ulcerative colitis.

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Section: Discussionmentioning

confidence: 70%

Section: Introductionmentioning

confidence: 96%

Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human Caco-2 cells

Maria-Ferreira

Nascimento

Cipriani

et al. 2018

Sci Rep

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“…The solutions were concentrated in a rotary evaporator under reduced pressure at [40][41][42][43][44][45] ∘ C, centrifuged at 5000-6000 rpm for 10-20 min, and lyophilized. Samples were lyophilized in frozen state using the VirTis lyophilizer (USA) at a constant vacuum of <10 mTorr and a temperature of −65 ∘ C. Samples were periodically removed and weighed to ensure constant weight after 6 h and dried for a longer period if the weight of the sample had changed more than 5% during the last 2 h of lyophilization.…”

Section: General Analytical Methodsmentioning

confidence: 99%

“…However, the pectins of tamarind [43], cactus cladode [44], and Brazilian jambu plant [45] have been shown to exhibit higher anti-DPPH activities (greater Journal of Chemistry 5 …”

Section: Dpph Radical Scavenging Activitymentioning

confidence: 99%

The Antioxidant Properties of Pectin Fractions Isolated from Vegetables Using a Simulated Gastric Fluid

Смирнов

Golovchenko

Vityazev

et al. 2017

Journal of Chemistry

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The antioxidant properties of vegetable pectin fractions against intraluminal reactive oxygen species were elucidated in vitro in conjunction with their structural features. The pectin fractions were isolated using a simulated gastric fluid (pH 1.5, pepsin 0.5 g/L, 37 ∘ C, 4 h) from fresh white cabbage, carrot, onion, and sweet pepper. The fraction from onion was found to inhibit the production of superoxide radicals by inhibiting the xanthine oxidase. The high molecular weight of onion pectin and a large number of galactose residues in its side chains appeared to participate in interaction with xanthine oxidase. All the isolated pectic polysaccharides were found to be associated with protein (2-9%) and phenolics (0.5-0.7%) as contaminants; these contaminants were shown to be responsible for the antioxidant effect of vegetable pectin fractions against the hydroxyl and 1,1-diphenyl-2-picrylhydrazyl radicals.

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“…Polysaccharides (pectines and arabinogalactans) from Aloe barbedensis [32], Panax ginseng [33,34], Acmella oleracea [35,36], Bupleurum falcatum [37,38].…”

Section: Mucopolysaccharides Layer (Physical) Protectionmentioning

confidence: 99%

Centaurea cyanus L. Polysaccharides and Polyphenols Cooperation in Achieving Strong Rat Gastric Ulcer Protection

et al. 2015

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This work was aimed at testing gastroprotective effects of Centaurea cyanus L. (herba) polysaccharides (P) and polyphenols (A) fractions on stress-induced rat ulcer model. Studies evaluating acute toxicity in rats and antioxidant (chemiluminescence method) and antimicrobial (on Staphylococcus aureus ATCC6538 and Escherichia coli ATCC8739 strains) activities of Centaurea cyanus L. (herba) product, which combined polysaccharides and polyphenols fractions (PA), have also been done. Accordingly, in vivo pharmacological studies revealed high influence of PA product (500 mg kg -1 of body weight) on deep, moderate and superficial gastric mucosal lesions, greater than that of chemical reference, Ranitidine. P product was proven more effective than Ranitidine in opposing the emergence of deep necrotic lesions only, suggesting the ability of polysaccharides compounds to consolidate gastric mucous layer as well as their certain tendency for cooperation with polyphenols fractions. The acute toxicity study indicated the lack of toxicity of PA product and maximum tolerated doses greater than 1875 mg kg -1 of body weight. PA product provided augmented scavenger activity and week antimicrobial activity on Staphylococcus aureus ATCC6538 and Escherichia coli ATCC8739 strains, resulting in better opportunities for valorisation of the aerial part of Centaurea cyanus L. species in order to obtain new and effective natural medicines.

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Rhamnogalacturonan from Acmella oleracea (L.) R.K. Jansen: Gastroprotective and Ulcer Healing Properties in Rats

Cited by 45 publications

References 40 publications

Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human Caco-2 cells

Rhamnogalacturonan, a chemically-defined polysaccharide, improves intestinal barrier function in DSS-induced colitis in mice and human Caco-2 cells

The Antioxidant Properties of Pectin Fractions Isolated from Vegetables Using a Simulated Gastric Fluid

Centaurea cyanus L. Polysaccharides and Polyphenols Cooperation in Achieving Strong Rat Gastric Ulcer Protection

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