RGD constructs with physical anchor groups as polymer co‐electrospinnable cell adhesives

Hommes-Schattmann, Paul J.; Neffe, Axel T.; Ahmad, Bilal; Williams, Gareth R.; Mbele, Gael; Menasché, Philippe; Kalfa, David; Lendlein, Andreas

doi:10.1002/pat.3963

Cited by 4 publications

(4 citation statements)

References 7 publications

(14 reference statements)

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“…Besides the possibility to display bioactive molecules on the scaffolds, a covalent chemical bond between the functionalizing entity and the scaffold is particularly useful to immobilize the active component on the scaffold surface. Poly( para ‐dioxanone) (PPDO) is well‐known as a suture in clinical use; however, it has not achieved a breakthrough in tissue engineering despite studies on tendon, bone, and vascular tissue engineering . The chemical structure of PPDO lacks suitable functional groups for specific covalent coupling of biologically active substrates, which makes surface functionalization by covalent reaction challenging.…”

Section: Introductionmentioning

confidence: 99%

“…vascular tissue engineering. [7][8][9][10][11][12][13] The chemical structure of PPDO lacks suitable functional groups for specific covalent coupling of biologically active substrates, which makes surface functionalization by covalent reaction challenging. Thus, establishing a method for covalent functionalization would offer a new prospective for PPDO in order to tailor its biointerface.…”

Section: Introductionmentioning

confidence: 99%

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Perfluorophenyl azide functionalization of electrospun poly(para‐dioxanone)

Luetzow

Hommes-Schattmann

Neffe

et al. 2018

Polymers for Advanced Techs

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Strategies to surface‐functionalize scaffolds by covalent binding of biologically active compounds are of fundamental interest to control the interactions between scaffolds and biomolecules or cells. Poly(para‐dioxanone) (PPDO) is a clinically established polymer that has shown potential as temporary implant, eg, for the reconstruction of the inferior vena cava, as a nonwoven fiber mesh. However, PPDO lacks suitable chemical groups for covalent functionalization. Furthermore, PPDO is highly sensitive to hydrolysis, reflected by short in vivo half‐life times and degradation during storage. Establishing a method for covalent functionalization without degradation of this hydrolyzable polymer is therefore important to enable the surface tailoring for tissue engineering applications. It was hypothesized that treatment of PPDO with an N‐hydroxysuccinimide ester group bearing perfluorophenyl azide (PFPA) under UV irradiation would allow efficient surface functionalization of the scaffold. X‐ray photoelectron spectroscopy and attenuated total reflectance Fourier‐transformed infrared spectroscopy investigation revealed the successful binding, while a gel permeation chromatography study showed that degradation did not occur under these conditions. Coupling of a rhodamine dye to the N‐hydroxysuccinimide esters on the surface of a PFPA‐functionalized scaffold via its amine linker showed a homogenous staining of the PPDO in laser confocal microscopy. The PFPA method is therefore applicable even to the surface functionalization of hydrolytically labile polymers, and it was demonstrated that PFPA chemistry may serve as a versatile tool for the (bio‐)functionalization of PPDO scaffolds.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Perfluorophenyl azide functionalization of electrospun poly(para‐dioxanone)

Luetzow

Hommes-Schattmann

Neffe

et al. 2018

Polymers for Advanced Techs

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“…Ring-opening polymerization has significant advantages, such as simple operation, mature technology, controllable product structure, and molecular weight, compared with the other chemical modification methods (Nikovia et al, 2019). Poly (p-dioxanone) (PPDO) has excellent mechanical strength, distinguished biodegradability, and biocompatibility, which are widely useful in surgical suturing, cardiovascular applications, and tissue engineering (Schattmann et al, 2017). Moreover, poly(lacticco-glycolic acid) (PLGA) is another popular biomedical degradable polymer material, which can regulate the comprehensive properties by changing the ratio of LLA to GA and is widely employed in tissue engineering (Yoo and Won, 2020;Song et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Biodegradable membrane of poly(l-lactide acid-dioxanone-glycolide) and stereocomplex poly(lactide) with enhanced crystallization and biocompatibility

Fan

Qin

Meng

et al. 2022

Front. Bioeng. Biotechnol.

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The membranes of poly(l-lactide acid-p-dioxanone-glycolide) (PLPG) with stereocomplex poly(lactic acid) (sc-PLA) were prepared by the solution blending way. It was observed that sc-PLA significantly heightened the crystallizing behavior of PLLA segments of the PLPG matrix. The crystallizing behavior displayed that the temperature of crystallization shifted to a higher range than that of PLPG. Moreover, the half-time of crystallization sharply decreased in value as the sc-PLA content increased in value on account of the pre-eminent nucleation ability of sc-PLA. TGA results revealed the thermal stability of the samples with the increase of sc-PLA compared to PLPG. Meanwhile, enzymatic degradation results indicated that the mass loss rate of the membrane decreased with the introduction of sc-PLA, but the overall degradation ability was still greater than that of PLLA. In the meantime, the biological experiment indicated that the membrane possessed low cytotoxicity.

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“…Different strategies were used to integrate peptides into electrospun fibers (EFs), including electrostatic attraction and bulk or surface modification [23]. The main drawback in using peptides/polymers blend or physically adsorbed peptides on ESP is the quick release of peptides [19] [24][25][26][27], which is often observed when the functionalized scaffolds are implanted in vivo [28]. To avoid this problem, their covalent link is more beneficial and affords more robust materials featuring mechanical properties similar to those shown by the native polymer.…”

Section: Introductionmentioning

confidence: 99%