RGD and BMP-2 mimetic peptide crosstalk enhances osteogenic commitment of human bone marrow stem cells

Abstract

For a long time, scientists thought that the differentiation of Mesenchymal Stem Cells (MSCs) into bone cells was dictated by growth factors. This manuscript shed light on other ligands that play a crucial role in regulating MSCs fate. In concrete terms, it was demonstrated that the osteoinductive effect of BMP-2 peptide is 2 folds improved in the presence of adhesive RGD peptide. Compared to previous works highlighting this synergistic cooperation between RGD and BMP-2 peptides, the main strength of this work lies to the use of primitive human cells (hMSCs) and well-defined biomimetic material surfaces (controlled surface roughness and peptide densities). This work provides valuable insights to develop custom-designed in vitro cell culture models, capable of targeting the desired cell response.

The spatial patterning of RGD and BMP‐2 mimetic peptides at the subcellular scale modulates human mesenchymal stem cells osteogenesis

“…The spatial patterning of peptides was ascertained at different stages, before and after peptide grafting and patterning. In a previous study, the covalent grafting of fluoro‐tagged RGD‐TAMRA and BMP‐2‐FITC peptides was evidenced using X‐ray photoelectron spectroscopy (XPS) . The peptides were randomly immobilized onto glass substrates.…”

“…Given that the surface roughness has been recognized for long time to greatly affect stem cells behavior and functions, we previously measured the surface roughness of RGD‐TAMRA and BMP‐2‐FITC peptides distributed in homogenous way on glass surface . Results showed no significant difference in the surface roughness between these two conditions.…”

“…To quantify the expression levels of fluorescently stained STRO‐1, Runx‐2, OPN markers, we used ImageJ freeware (NIH, http://rsb.info.nih.gov/ij/), as described in a previous work . Fluorescent images to be analyzed were acquired at the same exposure time and 40× magnification.…”

Chitosan/biphasic calcium phosphate scaffolds functionalized with BMP‐2‐encapsulated nanoparticles and RGD for bone regeneration

“…Advancements in bone tissue engineering require the improvement of tissue scaffolds that not only have large surface areas and highly inter‐connected porous structures with suitable porosity and pore size to support cell attachment and proliferation, but also carry signaling molecules that mediate cell activity . Biodegradable polymer scaffolds, such as chitosan (CS) and poly( l ‐lactide‐ co ‐glycolide) scaffolds, have been widely used because of their excellent biocompatibility and low toxicity.…”

Combinatorial Discovery of Defined Substrates That Promote a Stem Cell State in Malignant Melanoma

“…Growth factor derived peptides at high density have been shown to accommodate receptor activation similar to full-length proteins, while when presented at low density they may inhibit growth factor signaling. 27−30 These studies underscore the importance of understanding the relationships associated with ligand presentation and receptor signaling, when working with surface presenting short peptides.…”