The spatial patterning of RGD and BMP‐2 mimetic peptides at the subcellular scale modulates human mesenchymal stem cells osteogenesis

Bilem, Ibrahim; Laurent, Pascale; Chevallier, Pascale; Ayela, Cédric; Sone, Eli D.; Laroche, Gaétan; Durrieu, Marie‐Christine

doi:10.1002/jbm.a.36296

Cited by 23 publications

(43 citation statements)

References 60 publications

(141 reference statements)

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“…In one example, RGD and BMP peptides that promote cell adhesion and differentiation, respectively, have been micropatterned on glass substrates. 132 These substrates were fabricated by first functionalizing the surface so that it presented maleimide groups, upon which was coated a positive photoresist. Removal of the resist in the photoexposed areas revealed the maleimide groups that were used to capture thiol-presenting peptides.…”

Section: Applications Of Large-scale Lithographic Technologiesmentioning

confidence: 99%

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

2021

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Since the early 2000s, extensive research has been performed to address numerous challenges in biochip and biosensor fabrication in order to use them for various biomedical applications. These biochips and biosensor devices either integrate biological elements (e.g., DNA, proteins or cells) in the fabrication processes or experience post fabrication of biofunctionalization for different downstream applications, including sensing, diagnostics, drug screening, and therapy. Scalable lithographic techniques that are well established in the semiconductor industry are now being harnessed for large-scale production of such devices, with additional development to meet the demand of precise deposition of various biological elements on device substrates with retained biological activities and precisely specified topography. In this review, the lithographic methods that are capable of large-scale and mass fabrication of biochips and biosensors will be discussed. In particular, those allowing patterning of large areas from 10 cm 2 to m 2 , maintaining cost effectiveness, high throughput (>100 cm 2 h –1 ), high resolution (from micrometer down to nanometer scale), accuracy, and reproducibility. This review will compare various fabrication technologies and comment on their resolution limit and throughput, and how they can be related to the device performance, including sensitivity, detection limit, reproducibility, and robustness.

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Section: Applications Of Large-scale Lithographic Technologiesmentioning

confidence: 99%

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

2021

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“…Many clinical studies have demonstrated the excellent osteoinductivity of bone morphogenetic proteins (BMPs), especially BMP-2, but the direct use of BMP-2 in bone regeneration is hindered by its high cost and short half-life [5,6]. Therefore, the BMP-2-like osteogenic peptide, a safe and cost-effective synthetic osteoinductive peptide, has been increasingly used as a potent alternative to induce osteogenesis [7,8]. Generally, the rapid release and degradation of osteoinductive drugs compromise their function.…”

Section: Introductionmentioning

confidence: 99%

Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect

Zhang

Wang

et al. 2019

Molecules

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Osteogenic peptides have been reported as highly effective in directing mesenchymal stem cell osteogenic differentiation in vitro and bone formation in vivo. Therefore, developing novel biomaterials for the controlled delivery of osteogenic peptides in scaffolds without lowering the peptide’s biological activity is highly desirable. To repair a critical-sized bone defect to efficiently achieve personalized bone regeneration, a novel bioactive poly(lactic-co-glycolic acid) (PLGA)/β-tricalcium phosphate (β-TCP) composite scaffold, in which graphene oxide (GO) and bone morphogenetic protein (BMP)-2-like peptide were loaded in situ (PTG/P), was produced by an original cryogenic 3D printing method. The scaffolds were mechanically comparable to human cancellous bone and hierarchically porous. The incorporation of GO further improved the scaffold wettability and mechanical strength. The in situ loaded peptides retained a high level of biological activity for an extended time, and the loading of GO in the scaffold further tuned the peptide release so that it was more sustained. Our in vitro study showed that the PTG/P scaffold promoted rat bone marrow-derived mesenchymal stem cell ingrowth into the scaffold and enhanced osteogenic differentiation. Moreover, the in vivo study indicated that the novel PTG/P scaffold with sustained delivery of the peptide could significantly promote bone regeneration in a critical bone defect. Thus, the novel bioactive PTG/P scaffold with a customized shape, improved mechanical strength, sustainable peptide delivery, and excellent osteogenic ability has great potential in bone tissue regeneration.

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“…Given the biological synergy already observed between co-conjugated peptides on surfaces, in terms of differentiation, it is suggested that this synergistic behavior could also be observed with OGP as one of the conjugated peptides. 20,21 The goal of this study was therefore to develop surfaces with different peptides tethered onto the surface of PET material with a well-controlled density. PET material was chosen for its easy surface modification and as a model material.…”

Section: Introductionmentioning

confidence: 99%

Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage

Padiolleau

Chanseau

Durrieu

et al. 2018

ACS Appl. Bio Mater.

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The commitment and differentiation of human mesenchymal stem cells (hMSCs) are guided by bioactive molecules within the extracellular matrix. Among the various approaches to design biomaterials, the functionalization of biomaterial surfaces with peptides from the sequence of proteins from the extracellular matrix is quite common. The purpose of this functionalization is to recruit hMSCs and promote their differentiation into the appropriate lineage. The aim of this work was to investigate the influence of RGD and FHRRIKA peptides and peptide sequences taken from bone morphogenic protein (BMP-2) and histone H4 (osteogenic growth peptide; OGP) either tethered alone or as a mixture on the surface of a model material and to also examine the level of hMSC osteogenic commitment without using a differentiation medium. Grafting of the different peptides was assessed by X-ray photoelectron spectroscopy (XPS), while their surface density was quantified by fluorescence microscopy, and their surface properties were assessed by atomic force microscopy (AFM) and contact angle (CA). The osteogenic commitment of hMSCs cultured on the different surfaces was characterized by immunohistochemistry using Runx-2 as an earlier osteogenic marker and OPN, a late osteogenic marker, and by RT-qPCR through the expression of ColI-a1, Runx-2, and ALP. Biological results show that the osteogenic commitment of the hMSCs was increased on surfaces tethered with a mixture of peptides. Results indicate that tethered peptides in the range of pmol mm −2 were indeed effective in inducing a cellular response after 2 weeks of cell culture without using an osteogenic media. These findings contribute to the research efforts to design biomimetic materials able to induce a response in human stem cells through tethered bioactive molecules for bone tissue engineering.

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The spatial patterning of RGD and BMP‐2 mimetic peptides at the subcellular scale modulates human mesenchymal stem cells osteogenesis

Cited by 23 publications

References 60 publications

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

Lithographic Processes for the Scalable Fabrication of Micro- and Nanostructures for Biochips and Biosensors

Fabrication and Application of Novel Porous Scaffold in Situ-Loaded Graphene Oxide and Osteogenic Peptide by Cryogenic 3D Printing for Repairing Critical-Sized Bone Defect

Single or Mixed Tethered Peptides To Promote hMSC Differentiation toward Osteoblastic Lineage

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