RFLP analysis of SLA haplotypes in Swiss large white and American Hampshire pigs using SLA class I and class II probes

Shia, Yun Chao; Gautschi, C.; Ling, Ming Sheung; Beever, Jonathan E.; McLaren, David G.; Lewin, Harris A.; Schook, Lawrence B.

doi:10.1080/10495399109525749

Cited by 10 publications

(7 citation statements)

References 16 publications

(12 reference statements)

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“…Low correlation between serogical haplotypes and RFLP fragments has been reported for man (e.g. Driesel et al 1985), cattle (Lindberg & Andersson 1988;Oliver et al 1989) and was also indicated by earlier studies in pig (Chardon et al 1985;Shia et al 1991). It is obvious that these two methods detect polymorphisms in different regions of the class I genes or even in different genes of the class I gene region.…”

Section: Discussionmentioning

confidence: 69%

“…-). Furthermore, RFLP analysis with EcoRI of haplotypes H04, H01, H16 and H24 using long coding region probes (Chardon et al 1985;Shia et al 1991) detected 7, 8/9, 10 and 9/10 fragments, respectively.…”

Section: Discussionmentioning

confidence: 98%

“…The TaqI restriction enzyme was chosen to maximize the level of detectable polymorphism (e.g. Shia et al 1991) in the pig class I region. However, the number of detectable genes may be reduced if TaqI sites are numerous in the 39UT region homologous to the probe in this study.…”

Section: Discussionmentioning

confidence: 99%

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Variable number of pig MHC class I genes in different serologically defined haplotypes identified by a 3'-untranslated region probe

Ruohonen‐Lehto¹,

Renard²,

Rothschild³

et al. 1998

Animal Genetics

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To estimate the number of porcine class I major histocompatibility genes, a short class I cDNA probe from the 3'-untranslated region was developed to be used in restriction fragment length polymorphism analysis. Six clones isolated from a pig spleen cDNA library were sequenced from their 3'-untranslated region. Three different transcripts were identified, one probably derived from the class I PD7 locus and two showing highest homology to the PD1 and the PD14 genes, respectively. Class I typing was performed both by restriction fragment length polymorphism and serology. Segregation of class I haplotypes was followed in one three-generation family (European Wild Boar x Large White: Swedish Yorkshire) and in six two-generation families (Duroc, Yorkshire and Chester White), for a total of 266 pigs. Twenty different class I haplotypes were identified either with restriction fragment length polymorphism and/or serological typing. Furthermore, previously unpublished serological haplotypes H62, H67 and H68 were identified. Two to seven polymorphic and three monomorphic fragments were detected in different restriction fragment length polymorphism haplotypes indicating that the number of class I genes in the investigated haplotypes varies.

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Section: Discussionmentioning

confidence: 69%

Section: Discussionmentioning

confidence: 98%

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Variable number of pig MHC class I genes in different serologically defined haplotypes identified by a 3'-untranslated region probe

Ruohonen‐Lehto¹,

Renard²,

Rothschild³

et al. 1998

Animal Genetics

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“…The pdDRB DNA sequences share 78-82% similarity to human leucocyte antigen (HLA)-DRB, and pdDQB DNA sequence shows 80% (exon 2) and 87% (exon 3) identity to HLA DQB sequences. Southern blotrestriction fragment length polymorphism (RFLP) analyses showed DQB and DRB to be polymorphic in NIH miniature pigs (Sachs et al 1988) and in the Swiss Large White and American Hampshire breeds (Shia et al 1991). Five alleles for exon 2 Shia, Bradshaw, Rutherford, Le win, Schook The polymerase chain reaction (PCR)-RFLP typing method has been used to detect polymorphism at the second exon (PI) of the HLA-DQA1, -DQBl, -DRBZ, -DRB3, -DRB4 and -DRB5 loci (Uryu ef al.…”

Section: Introductionmentioning

confidence: 99%

Polymerase chain reaction based genotyping for characterization of SLA‐DQB and SLA‐DRB alleles in domestic pigs

et al. 1995

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Summary Molecular genotyping of swine major histocompatibility complex SLA‐DQB and SLA‐DRB genes using polymerase chain reaction (PCR)‐based amplification is described. Locus‐specific oligonucleotide primers were designed for the analysis of expressed SLA genes by reverse transcription‐polymerase chain reaction (RT‐PCR). RT‐PCR products were sequenced, and the information gained was used to design primers for PCR genotyping of the exon 2 (β1) region from genomic DNA templates. A single segregating amplification product was detected for both DQB and DRB in all animals. PCR products were digested with restriction enzymes. Seven SLA‐DQB PCR‐restriction fragment length polymorphism (RFLP) pattern types were observed for both HaeIII and Rsal that defined 14 SLA‐DQB alleles. A total of seven SLA‐DRB PCR‐RFLP pattern types were defined using Mspl (3 RFLP pattern types) and Rsal (6 RFLP pattern types). In order to demonstrate their universal utility, the primers were tested on genomic DNA samples from 10 different swine breeds. No breed‐specific alleles were observed. These results show that locus‐specific oligonucleotide primers and RFLP analysis provide a simple and rapid method for genotyping expressed SLA‐DQB and SLA‐DRB from genomic DNA.

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“…Among the peculiarities reported years ago are the remarkably small number of detectable MHC class I sequences. Depending on the techniques used, their extent varied from seven to ten per haplotype in miniature lines as well as in commercial breeds (8)(9)(10)(11). In comparison with pigs, humans and rodent species show several tens of class I sequences.…”

mentioning

confidence: 99%

A first map of the porcine major histocompatibility complex class I region

et al. 1998

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A map of the SLA complex, or swine major histocompatibility complex (MHC), class I region was constructed by alignment of yeast artificial chromosomes (YACs) harboring MHC class I genes as well as anchor genes already mapped within the human MHC complex (HLA). Five YACs containing 9 anchor genes built a contig of about 1.0–1.2 Mb between the SLA class HI BAT1 locus and the olfactory receptor‐like genes OLF42. Ten different SLA class I sequences, including putative allelic forms of published classical and non‐classical SLA class I genes, were assigned to the 400‐kb enclosing centromeric part of the contig. Three additional YACs comprising the OLF89 genes and two YACs containing the butyrophilin gene were located telomeric to the contig. Comparison between the human and porcine MHC complexes showed a perfect conserved order of anchor genes, whereas no orthologous relationships were found for the class I loci.

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RFLP analysis of SLA haplotypes in Swiss large white and American Hampshire pigs using SLA class I and class II probes

Cited by 10 publications

References 16 publications

Variable number of pig MHC class I genes in different serologically defined haplotypes identified by a 3'-untranslated region probe

Variable number of pig MHC class I genes in different serologically defined haplotypes identified by a 3'-untranslated region probe

Polymerase chain reaction based genotyping for characterization of SLA‐DQB and SLA‐DRB alleles in domestic pigs

A first map of the porcine major histocompatibility complex class I region

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