Variable number of pig MHC class I genes in different serologically defined haplotypes identified by a 3'-untranslated region probe

Ruohonen‐Lehto, M. K.; Renard, C.; Rothschild, M. F.; Edfors-Lilja, I.; Kristensen, B.; Gustafsson, U.; Larson, R. G.; Varvio, S.-L.

doi:10.1111/j.1365-2052.1998.00308.x

Cited by 10 publications

(2 citation statements)

References 11 publications

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“…Indeed, the general organization of the axolotl MHC, with the presence of many class I genes, is not restricted to this species since it is also found in A. tigrinum [34]. Moreover, a variable number of MHC loci per haplotype can be found in several organisms including class I in mouse [37], cow [5], pig [38] and fish and human class II B [1,39,40]. This could create a new type of polymorphism for MHC molecules.…”

Section: Discussionmentioning

confidence: 99%

Axolotl MHC architecture and polymorphism

Sammut¹,

Pasquier²,

Ducoroy

et al. 1999

Eur. J. Immunol.

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The MHC of the urodele amphibian Ambystoma mexicanum consists of multiple polymorphic class I loci linked, so far as yet known, to a single class II B locus. This architecture is very different from that of the anuran amphibian Xenopus. The number of class I loci in the axolotl can vary from 6 to 21 according to the haplotypes as shown by cDNA analysis and Southern blot studies in families. These loci can be classified into seven sequence groups with features ranging from the class Ia to the class Ib type. All individuals express genes from at least three of the seven groups, and all individuals possess the class Ia‐like type.

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Section: Discussionmentioning

confidence: 99%

Axolotl MHC architecture and polymorphism

Sammut¹,

Pasquier²,

Ducoroy

et al. 1999

Eur. J. Immunol.

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“…The number of expressed SLA class I molecules is presumed to be flexible according to the haplotypes. Although no formal demonstration has been performed until now, analyses of restriction fragment length polymorphism (RFLP) suggested that the size of the class I region and the number of class I sequences differ in various haplotypes (Ruohonen-Lehto et al 1998). More recently, transcript sequencing indicated that a duplicated SLA-1 locus could be found for three haplotypes (Smith et al 2005b).…”

Section: Variation In Number Of the Sla Locusmentioning

confidence: 99%

Gene organization and polymorphism of the swine major histocompatibility complex

Andõ

Chardon

2006

Animal Science Journal

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The recent availability of the full‐length sequence of one haplotype of the swine leukocyte antigen (SLA) complex, the swine major histocompatibility complex (MHC), and significant progress in the studies on gene expression and polymorphisms led to major advances in deciphering its role in resistance to diseases in animals. The present status of the genomic organization and polymorphism of the SLA complex is presented in this Review. Additionally, a comparative analysis with mammalian MHC has also been provided. The sequenced SLA‐H01 haplotype harbors 152 loci including genuine SLA genes, non‐MHC genes and pseudogenes. Although the numbers of expressed SLA genes could vary across haplotypes, three SLA class Ia, three SLA class Ib, four SLA class IIa and four SLA class IIb genes are currently expressed. Except for the class I genes, which have no clear orthologs, the gene organization of the loci was highly conserved between humans and pigs. Moreover, the human leukocyte antigen (HLA) complex lies on a single chromosomal segment, whereas a centromere at the class II and III junction splits the SLA complex into two segments, without disturbing gene organization or impeding functionality. Over 400 SLA class I and II allele sequences available in databases have been recently clustered and assigned to a specific SLA locus according to a newly defined nomenclature system.

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Haplotype characterization of transcribed ovine major histocompatibility complex (MHC) class I genes

et al. 2005

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The ovine major histocompatibility complex (MHC) remains poorly characterized compared with those of other livestock species. Molecular genetic analysis of the bovine MHC has revealed considerable haplotype and allelic diversity that earlier serological analysis had not detected. To develop cellular and molecular tools to support development of vaccines against intracellular pathogens of sheep, we have undertaken a molecular genetic analysis of four distinct ovine MHC haplotypes carried by two heterozygous Blackface rams. We have identified 12 novel class I transcripts and used a class I sequence-specific genotyping system to assign each of these transcripts to individual haplotypes. Using a combination of phylogenetic analysis, haplotype and transcript expression data, we identified at least four distinct polymorphic class I MHC loci, three of which appear together in a number of combinations in individual haplotypes. The haplotypes were further characterized at the highly polymorphic Ovar-DRB1 locus, allowing selection of the progeny of the two founder rams for the establishment of an MHC-defined resource population.

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Variable number of pig MHC class I genes in different serologically defined haplotypes identified by a 3'-untranslated region probe

Cited by 10 publications

References 11 publications

Axolotl MHC architecture and polymorphism

Axolotl MHC architecture and polymorphism

Gene organization and polymorphism of the swine major histocompatibility complex

Haplotype characterization of transcribed ovine major histocompatibility complex (MHC) class I genes

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