Rewritable DNA Microarrays

Schofield, W. C. E.; McGettrick, James D.; Bradley, T. J.; Badyal, J. P. S.; Przyborski, Stefan

doi:10.1021/ja056367r

Cited by 54 publications

(63 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared to conventional aldehyde-bearing slides based on SAMs, which are highly compact (rigid) in nature, the steric flexibility of plasma-chemical-functionalized layers is clearly an advantageous factor. [29,40] Biologists have recognized the need for flexible linker layers to improve hybridization efficiency, reporting that even linkers of <28 Å can increase hybridization fourfold, [46] whilst linkers >40 Å offer a 150-fold increase in hybridization efficiency. [47] Furthermore, in the present study, the plasmachemical-functionalized aldehyde layers give rise to good DNA-DNA recognition, as demonstrated by the high selectivity of complementary versus non-complementary hybridization.…”

Section: Discussionmentioning

confidence: 99%

“…Examples devised in the past include; epoxide-, [20] anhydride-, [22] carboxylic acid-, [23] cyano-, [24] hydroxyl-, [25] furfuryl-, [26] amine-, [27] pyridine-, [28] thiol-, [29] perfluoroalkyl-, [30] perfluoromethylene-, [31] and trifluoromethyl- [32] functionalized surfaces. Although previous attempts have been made to plasma-deposit aldehyde groups from precursors such as acrolein, [33][34][35] acetaldehyde, [36] or benzaldehyde, [37] they have all suffered from poor aldehyde group incorporation due to either extensive fragmentation of the monomer or the absence of a polymerizable carboncarbon double bond.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Substrate‐Independent Approach for the Surface Immobilization of Oligonucleotides using Aldehyde Functionalized Surfaces

McGettrick

Schofield

Garrod

et al. 2009

Chemical Vapor Deposition

View full text Add to dashboard Cite

A highly efficient approach for the covalent immobilization of single-strand DNA onto solid surfaces and their subsequent hybridization to complementary strands is described. This entails pulsed plasma deposition of poly(3-vinylbenzaldehyde) followed by the Schiff-base reaction. A range of substrates, including glass slides, silicon wafers, polystyrene microspheres, and single-walled carbon nanotubes (SWNTs), are functionalized employing this methodology.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A Substrate‐Independent Approach for the Surface Immobilization of Oligonucleotides using Aldehyde Functionalized Surfaces

McGettrick

Schofield

Garrod

et al. 2009

Chemical Vapor Deposition

View full text Add to dashboard Cite

show abstract

“…As expected, this feature was absent for both 1-propanethiol monomer and its corresponding plasma polymer. Both 1-propanethiol and allyl mercaptan precursors show a weak S-H stretch (2555 cm -1 ), 40,41 which is also observed for plasma deposited allyl mercaptan; however it was absent for plasma deposited 1-propanethiol. 20,42,43 : firstly there is the plasma deposited layer thiol groups reacting with carbon-carbon alkene bonds present within the underlying polymer base layer (e.g.…”

Section: Plasma Deposited Top Layermentioning

confidence: 91%

Plasmachemical Double Click Thiol–ene Reactions for Wet Electrical Barrier

Fraser

Carletto

Wilson

et al. 2016

ACS Appl. Mater. Interfaces

Self Cite

View full text Add to dashboard Cite

The full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. ABSTRACTClick thiol-ene chemistry is demonstrated for the reaction of thiol containing molecules with surface alkene bonds during electrical discharge activation. This plasmachemical reaction mechanism is shown to be two-fold for allyl mercaptan (an alkene and thiol group containing precursor), comprising self-crosslinked nanolayer deposition in tandem with interfacial crosslinking to the surface alkene bonds of a polyisoprene base layer. A synergistic multilayer structure is attained which displays high wet electrical barrier performance during immersion in water.4

show abstract

“…Changes in electrochemical signals through an external sensing device were detected quantitatively. 26,27 As a result, the bio-MEMS-based microbiochip was successfully produced and consisted of desired electrodes in the system. Samples which can be detected with the help of a DNA probe were easily immobilized onto the microbiochip.…”

Section: Introductionmentioning

confidence: 99%

Development of Electrochemical Microbiochip for the Biological Diagnosis of Neisseria gonorrhoeae

et al. 2013

View full text Add to dashboard Cite

A sexually transmitted disease is an illness that has a high probability of transmission between humans or animals who have sexual contact. Our research is based on the development of a microbiochip for Neisseria gonorrhoeae (N.G.). In our study, we have employed fusion technology between microarray technology and a microfluidic system for quantitative analysis of N.G. A great deal of attention has been focused on electrochemical detection by using a DNA probe, which is a specific DNA sequence and binds to a target biomolecule, because of high affinity, ease of usage, and fast measurement. The microbiochip consisted of two electrode systems and microchannel based PDMS. Our detection principles use electrochemical detection. Consequently, our microbiochip detected 5 ng/mL of N.G. and the correlation rate was over 0.95. We can produce a microbiochip, which could bind to a DNA probe and detect sample of interest. We expect that our electrobiochemical chip will be used for the development of a portable device.

show abstract

Rewritable DNA Microarrays

Cited by 54 publications

References 49 publications

A Substrate‐Independent Approach for the Surface Immobilization of Oligonucleotides using Aldehyde Functionalized Surfaces

A Substrate‐Independent Approach for the Surface Immobilization of Oligonucleotides using Aldehyde Functionalized Surfaces

Plasmachemical Double Click Thiol–ene Reactions for Wet Electrical Barrier

Development of Electrochemical Microbiochip for the Biological Diagnosis of Neisseria gonorrhoeae

Contact Info

Product

Resources

About